The last two decades have seen the rapid expansion of click chemistry methodology in various domains closely related to organic chemistry. It has notably been widely developed in the area of surface chemistry, mainly because of the high-yielding character of reactions of the "click" type. Especially, this powerful chemical reaction toolbox has been adapted to the preparation of stationary phases from the corresponding chromatographic supports. A plethora of selectors can thus be immobilized on either organic, inorganic, or hybrid stationary phases that can be used in different chromatographic modes. This review first highlights the few different chemical ligation strategies of the "click" type that are up to now mainly devoted to the development of functionalized supports for separation sciences. Then, it gives in a second part an up-to-date survey of the different studies dedicated to the preparation of click chemistry-based chromatographic supports while highlighting the powerful and versatile character of the "click" ligation strategy for the design, synthesis, and developments of more and more complex systems that can find promising applications in the area of analytical sciences, in domains as varied as enantioselective separation, glycomics, proteomics, genomics, metabolomics, etc.