Preparation and evaluation of a macroporous molecularly imprinted hybrid silica monolithic column for recognition of proteins by high performance liquid chromatography

Lin, Zian; Yang, F.; He, Xiwen; Zhao, Xiaomiao; Zhang, Yukui

doi:10.1016/j.chroma.2009.10.025

Cited by 84 publications

(46 citation statements)

References 38 publications

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“…[67][68][69] Monolithic MIPs have also been prepared by a simple, onestep, in situ, free-radical polymerization process within the confines of a chromatographic column without the need for grinding, sieving and column packing. 70,71 By using silane chemistry or surface ''grafting to'' approaches, membranes and molecularly imprinted monolayers are generally formed on an appropriate support of fibers or films. In addition to the polymerization techniques mentioned above, electrochemical polymerization, electrodeposition, grafting on monolithic columns, photografting, and sol-gel methods have also been employed.…”

Section: Preparation Proceduresmentioning

confidence: 99%

Molecular imprinting: perspectives and applications

et al. 2016

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a Molecular imprinting technology (MIT), often described as a method of making a molecular lock to match a molecular key, is a technique for the creation of molecularly imprinted polymers (MIPs) with tailor-made binding sites complementary to the template molecules in shape, size and functional groups. Owing to their unique features of structure predictability, recognition specificity and application universality, MIPs have found a wide range of applications in various fields. Herein, we propose to comprehensively review the recent advances in molecular imprinting including versatile perspectives and applications, concerning novel preparation technologies and strategies of MIT, and highlight the applications of MIPs. The fundamentals of MIPs involving essential elements, preparation procedures and characterization methods are briefly outlined.Smart MIT for MIPs is especially highlighted including ingenious MIT (surface imprinting, nanoimprinting, etc.), special strategies of MIT (dummy imprinting, segment imprinting, etc.) and stimuli-responsive MIT (single/dual/ multi-responsive technology). By virtue of smart MIT, new formatted MIPs gain popularity for versatile applications, including sample pretreatment/chromatographic separation (solid phase extraction, monolithic column chromatography, etc.) and chemical/biological sensing (electrochemical sensing, fluorescence sensing, etc.). Finally, we propose the remaining challenges and future perspectives to accelerate the development of MIT, and to utilize it for further developing versatile MIPs with a wide range of applications (650 references).

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Section: Preparation Proceduresmentioning

confidence: 99%

Molecular imprinting: perspectives and applications

et al. 2016

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“…Molecular imprinting is a powerful technique for constructing a synthetic polymer receptor with tailor-made binding sites, that is, molecularly imprinted polymers (MIPs), which are highly cross-linked polymer containing spatial and functionality memory for template molecules, and provide the specific selectivity when used as molecular selective stationary phases in chromatography [96,97]. Monolithic MIPs can be prepared in a capillary or stainless steel column either by in situ copolymerization of functional monomers and crosslinkers in the presence of template molecules [98] or by post-grafting of MIPs on the silica [99] or organic-silica hybrid [100,101] monolithic matrices. A novel approach of room temperature ionic liquid (RTIL)-mediated non-hydrolytic sol-gel (NHSG) was explored for the fabrication of new molecularly imprinted hybrid monoliths for CEC enantioseparation by Yan's group [102] using MAA and ␥-MAPS as precursors.…”

Section: Microscale Separationmentioning

confidence: 99%

Recent advances in preparation and application of hybrid organic‐silica monolithic capillary columns

Lin

Zhang

et al. 2012

Electrophoresis

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Hybrid organic-silica monolithic columns, regarded as a second generation of silica-based monoliths, have received much interest due to their unique properties over the pure silicabased monoliths. This review mainly focuses on development in the fields of preparation of hybrid monolithic columns in a capillary and their application for CEC and capillary liquid chromatography separation, as well as for sample pretreatment of solid-phase microextraction and immobilized enzyme reactor since July 2010. The preparation approaches are comprehensively summarized with three routes: (i) general sol-gel process using trialkoxysilanes and tetraalkoxysilanes as coprecursors; (ii) "one-pot" process of alkoxysilanes and organic monomers concomitantly proceeding sol-gel chemistry and free radical polymerization; and (iii) other polymerization approaches of organic monomers containing silanes. The modification of hybrid monoliths containing reactive groups to acquire the desired surface functionality is also described.

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“…Over the past decades, molecularly imprinted polymer (MIP) has been successfully applied to a variety of small molecules [3,4]. Recent years, much attention has been paid to the imprinting of biomacromolecules and especially protein for its potential applications as biomaterials in separation, purification, biosensor, and mimicking enzyme and antibody [5][6][7][8][9][10][11]. However, the large molecular size, complexity, conformational flexibility, solubility, and numbers of functional groups of the protein make its imprinting face great challenges.…”

Section: Introductionmentioning

confidence: 99%