Preparation and evaluation of biomimetric nano-hydroxyapatite-based composite scaffolds for bone-tissue engineering

Abstract

In the present study, novel biomimetic composite scaffolds with a composition similar to that of natural bone were prepared, using nano-hydroxyapatite, collagen, and phosphatidylserine. The scaffolds possess an interconnected porous structure with a porosity of 84%. The pore size ranges from several micrometers up to about 400 m. In-vitro studies in simulated body fluids showed that the morphologies of the products derived from mineralization can be regulated by the extracellular matrix components of the scaffolds; this in turn leads to creation of a large number of hydroxyapatite crystals on the scaffold surface. The regulatory properties of collagen and phosphatidylserine also influenced the cell response to the composite scaffolds. MC3T3-E1 cells attached and spread on the surfaces of the materials and interacted with the substrates; this may be the result of charged groups on the composite materials. Radiological analysis suggested that calluses and bone bridges formed in defects within 12 weeks. These composite scaffolds may therefore be a suitable replacement in bone-tissue engineering.composite, scaffold, cell, biomineralization, regulatory Citation:Yang C R, Wang Y J, Chen X F. Preparation and evaluation of biomimetric nano-hydroxyapatite-based composite scaffolds for bone-tissue engineering.

Microporous Nano-hydroxyapatite/collagen/phosphatidylserine Scaffolds Embedding Collagen Microparticles for Controlled Drug Delivery in Bone Tissue Engineering

“…The inorganic phase consists primarily of hydroxyapatite (HA), whilst the organic phase consists mainly of type I collagen (COL1) and small amounts of ground substance, such as glycosaminoglycans, proteoglycans, glycoproteins and phosphatidylserine (PS), all with unique distributions reflecting their biological roles. HA, COL and PS, when processed into biomaterial, results a bone scaffold with appropriate bio-degradation, excellent mechanical properties, and well bioactivity and biocompatibility, which has been demonstrated in our previous work 8 . Here we introduce a protocol to fabricate inhomogeneous HA/COL/PS (HCP) scaffolds with gradient distribution of SS loaded collagen microparticles.…”

Cytocompatible 3D chitosan/hydroxyapatite composites endowed with antibacterial properties: toward a self-sterilized bone tissue engineering scaffold

“…The peaks between 960 and 1,100 cm -1 are considered to be the major n3 vibration peaks of PO 4 3- [36], while the peaks between 2,500 and 3,500 cm -1 are attributed to C-H and O-H groups in the chitosan and HAp. The band between 1,400 and 1,430 cm -1 indicates the existence of carbonated HAp, which means that a small amount of the PO 4 3-or OHgroups had been replaced by CO 3 2-ions and formed Ca 10-x (PO 4 ) 6-x (CO 3 ) x (OH) 2-x (with 0 \ x \ 2) in the HAp nano-structures [6,37]. The intensity of these absorption bands decreases with the increase of silver nitrate concentration added for ion substitution.…”

Silk fibroin/hydroxyapatite scaffold: a highly compatible material for bone regeneration

“…When composited with HA, collagen shows cell differentiation as well. Collagen contains several kinds of negatively charged, positively charged, and polar yet neutral groups, in which the negatively charged groups undergo chemical reactions that suppress the nucleation of HA on collagen [135][136][137][138][139][140][141][142][143][144][145][146][147][148][149][150][151].…”