Preparation and Evaluation of Ricinus communis Agglutinin Affinity Adsorbents Using Polymeric Supports

Cartellieri, Simone; Helmholz, Heike; Niemeyer, Bernd

doi:10.1006/abio.2001.5177

Cited by 21 publications

(14 citation statements)

References 22 publications

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“…?RCA is a glycoprotein from seeds of castor plants and one of the most important applied lectins that have been widely used as a tool to study cell surfaces and to purify glycans [79]. RCA promotes binding and agglutination of polysaccharides and glycoproteins in addition to liposomes and micelles containing glycolipids with galactosyl residues [80], [81]. Furthermore, the specificities of interactions of RCA with neutral and sialylated oligosaccharides have been well established and is consistent with our results as summarized in Table S11 [82].…”

Section: Resultssupporting

confidence: 89%

Community-Based Network Study of Protein-Carbohydrate Interactions in Plant Lectins Using Glycan Array Data

Malik

Lee

2014

PLoS ONE

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Lectins play major roles in biological processes such as immune recognition and regulation, inflammatory responses, cytokine signaling, and cell adhesion. Recently, glycan microarrays have shown to play key roles in understanding glycobiology, allowing us to study the relationship between the specificities of glycan binding proteins and their natural ligands at the omics scale. However, one of the drawbacks in utilizing glycan microarray data is the lack of systematic analysis tools to extract information. In this work, we attempt to group various lectins and their interacting carbohydrates by using community-based analysis of a lectin-carbohydrate network. The network consists of 1119 nodes and 16769 edges and we have identified 3 lectins having large degrees of connectivity playing the roles of hubs. The community based network analysis provides an easy way to obtain a general picture of the lectin-glycan interaction and many statistically significant functional groups.

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Section: Resultssupporting

confidence: 89%

Community-Based Network Study of Protein-Carbohydrate Interactions in Plant Lectins Using Glycan Array Data

Malik

Lee

2014

PLoS ONE

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“…The synthesis of aldehyde modified silica material was carried out using the previously reported reductive amination reaction [25]. In brief, to a solution of dried silica gel (30 nm, 2.50 g) in 30 mL anhydrous toluene containing GPMS (2.9 mmol, 0.82 mL) was added equivalent pyridine (100 L), which was allowed to proceed at 80 • C for 48 h. The resulting epoxy silica 8 was then filtered, washed successively with methanol, water and methanol, and dried at 80 • C overnight.…”

Section: Preparation Of the Sio 2 -Ald Con A Materialsmentioning

confidence: 99%

“…For comparison, another silica-based Con A material (SiO 2 -Ald Con A) was prepared using conventional (3-glycidoxypropyl) triethoxysilane (GPMS) as the initial spacer arm [25]. Both the synthesized materials were characterized by elemental analysis, Fourier transform infrared spectrometry (FT-IR) and zeta potential measurements.…”

Section: Introductionmentioning

confidence: 99%

Oxidized dextran facilitated synthesis of a silica-based concanavalin a material for lectin affinity enrichment of glycoproteins/glycopeptides

Liu

et al. 2016

Journal of Chromatography A

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“…The preparation of lectin-affinity adsorbents was performed as described previously [23,35]. Immobilization of ConA onto tresylated polymer supports was carried out within 4 h using 0.5 M phosphate buffer (pH 8.0) and the remaining tresyl groups were blocked with 0.2 M Tris-HCl buffer (pH 8.0).…”

Section: Preparation and Characterization Of Affinity Adsorbentsmentioning

confidence: 99%

Isolation of a cytotoxic glycoprotein from the Scyphozoa Cyanea lamarckii by lectin-affinity chromatography and characterization of molecule interactions by surface plasmon resonance

Helmholz¹,

Naatz

Lassen³

et al. 2008

Journal of Chromatography B

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A biospecific lectin-affinity-based isolation process for a novel glycoprotein (ClGp1) from the venom of the pelagic jellyfish Cyanea lamarckii, is described and the isolated glycoprotein is chemically and biologically characterized according to size, molecular interaction and toxicity. The molecular mass of the isolated protein is 25.7 kDa as determined by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF). The carbohydrate content was calculated after enzymatic deglycosylation as 6.85 kDa. The glycoprotein is cytotoxic and could be isolated from cnidocysts of mesenteric and fishing tentacles. The binding behaviour of the glycoprotein to the lectins Concanavalin A (ConA) and Wheat Germ Agglutinin (WGA) was analyzed by surface plasmon resonance (SPR) and affinity constants in the range of K(D)=3.0 x 10(-7) M for ConA and 2.1 x 10(-6) M (pH 5.0) and 2.6 x 10(-6) M (pH 7.4) for WGA were obtained.

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Preparation and Evaluation of Ricinus communis Agglutinin Affinity Adsorbents Using Polymeric Supports

Cited by 21 publications

References 22 publications

Community-Based Network Study of Protein-Carbohydrate Interactions in Plant Lectins Using Glycan Array Data

Community-Based Network Study of Protein-Carbohydrate Interactions in Plant Lectins Using Glycan Array Data

Oxidized dextran facilitated synthesis of a silica-based concanavalin a material for lectin affinity enrichment of glycoproteins/glycopeptides

Isolation of a cytotoxic glycoprotein from the Scyphozoa Cyanea lamarckii by lectin-affinity chromatography and characterization of molecule interactions by surface plasmon resonance

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