Preparation and functional characterization of tumor-targeted folic acid-chitosan conjugated nanoparticles loaded with mitoxantrone

Wang, Wei; Tong, Chunyi; Liu, Xingyan; Li, Tao; Liu, Bin; Xiong, Wei

doi:10.1007/s11771-015-2871-5

Cited by 25 publications

(12 citation statements)

References 21 publications

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“…Due to the reaction between the carboxyl group of FA and the amide of CS, the peak of the carboxyl group at ~1686 cm −1 disappeared, while the peak of the amide shifted to ~1644 cm −1 and overlapped with the peak of the new C-N bond [33]. Further shifts were observed following the conjugation of the CS-FA and His to AuNPs (Figure 2D), with a characteristic peak at 1606 cm −1 due to the benzene ring of FA [46]. High-resolution transmission electron microscopy (HRTEM) was utilized to visualize the ultrastructural characteristics of the NPs and are represented in Figure 3.…”

Section: Nanoparticle Synthesis and Characterizationmentioning

confidence: 96%

Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

et al. 2021

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Nanotechnology has emerged as a promising treatment strategy in gene therapy, especially against diseases such as cancer. Gold nanoparticles (AuNPs) are regarded as favorable gene delivery vehicles due to their low toxicity, ease of synthesis and ability to be functionalized. This study aimed to prepare functionalized AuNPs (FAuNPs) and evaluate their folate-targeted and nontargeted pCMV-Luc-DNA delivery in breast cancer cells in vitro. CS was added to induce stability and positive charges to the AuNPs (Au-CS), histidine (Au-CS-His) to enhance endosomal escape and folic acid for folate-receptor targeting (Au-CS-FA-His). The FAuNP:pDNA nanocomplexes possessed favorable sizes (<135 nm) and zeta potentials (<−20 mV), strong compaction efficiency and were capable of pDNA protection against nuclease degradation. These nanocomplexes showed minimal cytotoxicity (>73% cell viability) and enhanced transgene activity. The influence of His was notable in the HER2 overexpressing SKBR3 cells, which produced higher gene expression. Furthermore, the FA-targeted nanocomplexes enhanced receptor-mediated endocytosis, especially in MCF-7 cells, as confirmed by the receptor competition assay. While the role of His may need further optimization, the results achieved suggest that these FAuNPs may be suitable gene delivery vehicles for breast cancer therapeutics.

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Section: Nanoparticle Synthesis and Characterizationmentioning

confidence: 96%

Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

et al. 2021

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“…2d). Spherical shaped particles, which are 100–200 nm in size, had the very best capacity for the extended movement; quickly and consistently disguised on account of their symmetry (Petros and Desimone, 2010, Udompornmongkol and Chiang, 2016, Wang et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial dysfunction mediated apoptosis of HT-29 cells through CS-PAC-AgNPs and investigation of genotoxic effects in zebra (Danio rerio) fish model for drug delivery

Suganya¹,

Gnanamangai²,

Govindasamy

et al. 2019

Saudi Journal of Biological Sciences

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The present study reports the validation of cancer nanotherapy using proanthocyanidin (PAC). Nowadays, in vitro and in vivo deliveries of nanoparticle (NPs) drugs have been paid more attention, intensively. Moreover, the current chemotherapeutic drugs have few first rate drawbacks including lack of specificity and requirement of excessive drug doses. To overcome this problem of chemotherapy, the attainment of high drug loading in combination with degradable polymer nanoparticles (for instance,chitosan) is a trending research in cancer biology. Hence, in this study, the synthesized PAC-AgNPs were successfully crosslinked with chitosan nanoparticles (CS-PAC-AgNPs), which were found to be spherical or polygonal in shape with a median size of 70.68 nm and 52.16 nm as observed by FTIR, FESEM and TEM analysis; thus, being suitable for drug delivery. CS-PAC-AgNPs were taken up via endocytosis by cancer cells and enabled the release cytochrome-C from mitochondria, followed by dysregulation of anti-apoptotic protein Bcl2 family, inducing the apoptotic mediated activation of caspase 9 and 3. To identify the genotoxicity of the synthesized CS-PAC-AgNPs, the mortality, hatching rate, malformation and abnormalities of embryo/larvae of the vertebrate zebra fish model ( Danio rerio ) were observed in a dose-time-dependent manner. This improved cancer nanotherapy can thus be utilized as a novel nanocombination for inducing apoptosis in vitro and in vivo .

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“…Further, folic acidchitosan conjugate, dissolved in acetate buffer (pH 5.6) reacted with N-hydroxysuccinimide-ester of folic acid to form folic acidchitosan conjugate. Similarly, Wang et al [19] synthesized folic acid-chitosan conjugate, while loading mitoxantrone into folic acid-chitosan conjugated nanoparticles. Naghibi Beidokhti et al [20] synthesized folic acid-chitosan conjugate by reaction of N-hydroxysuccinimide ester of folic acid with chitosan.…”

Section: Resultsmentioning

confidence: 99%

“…Earlier, Salar and Kumar [14] observed spherical shaped vincristine loaded folic acid-chitosan conjugated nanoparticles with granule like structure due to loading of vincristine. Similarly, spherical shaped mitoxantrone loaded folic acid-chitosan conjugated nanoparticles has also been observed [19].…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 88%

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Synthesis, characterization and anticancer activity of vincristine loaded folic acid-chitosan conjugated nanoparticles on NCI-H460 non-small cell lung cancer cell line

Kumar

Salar

Prasad

et al. 2018

Egyptian Journal of Basic and Applied Sciences

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Preparation and functional characterization of tumor-targeted folic acid-chitosan conjugated nanoparticles loaded with mitoxantrone

Cited by 25 publications

References 21 publications

Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

Histidine-Tagged Folate-Targeted Gold Nanoparticles for Enhanced Transgene Expression in Breast Cancer Cells In Vitro

Mitochondrial dysfunction mediated apoptosis of HT-29 cells through CS-PAC-AgNPs and investigation of genotoxic effects in zebra (Danio rerio) fish model for drug delivery

Synthesis, characterization and anticancer activity of vincristine loaded folic acid-chitosan conjugated nanoparticles on NCI-H460 non-small cell lung cancer cell line

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