Preparation and<i>in vitro</i>characterization of pluronic-attached polyamidoamine dendrimers for drug delivery

Gu, Zhuo-Jun; Wang, Meng; Fang, Qiongyan; Zheng, Huiyuan; Feiyue, Wu; Lin, Dai; Xu, Ying; Jin, Yi

doi:10.3109/03639045.2014.908899

Cited by 27 publications

(8 citation statements)

References 29 publications

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“…Similar association was previously observed by AFM imaging for nimesulide-containing megamer G3 12G024Nim , which showed 5.1 nm size measured by DLS for dilute solution, while average height of associates deposited on mica was 9 nm [12]. The molecular association also might be responsible for reported large size (185 nm) of PAMAM G5 measured by DLS [28]. In the case of the highly loaded cytisine conjugate, G3 9C11gh , as well as in the mentioned nimesulide megamer, hydrophobic association between aromatic parts of C (and Nim) is responsible for association.…”

Section: Determination Of Conjugate Size By Dynamic Light Scattering supporting

confidence: 86%

Stepwise Glucoheptoamidation of Poly(Amidoamine) Dendrimer G3 to Tune Physicochemical Properties of the Potential Drug Carrier: In Vitro Tests for Cytisine Conjugates

et al. 2020

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Third-generation poly(amidoamine) dendrimer (PAMAM) was modified by stepwise primary amine group amidation with d-glucoheptono-1,4-lactone. The physicochemical properties of the conjugates—size, ζ potential in lysosomal pH 5 and in neutral aqueous solutions, as well as intramolecular dynamics by differential scanning calorimetry—were determined. Internalization and toxicity of the conjugates against normal human fibroblasts BJ were monitored in vitro in order to select an appropriate carrier for a drug delivery system. It was found that initial glucoheptoamidation (up to 1/3 of amine groups of neat dendrimers available) resulted in increase of conjugate size and ζ potential. Native or low substituted dendrimer conjugates accumulated efficiently in fibroblast cells at nontoxic 1 µM concentration. Further substitution of dendrimer caused consistent decrease of size and ζ potential, cell accumulation, and toxicity. All dendrimers are amorphous at 36.6 °C as determined by differential scanning calorimetry (DSC). The optimized dendrimer, half-filled with glucoheptoamide substituents, was applied as carrier bearing two covalently attached cytisine molecules: a rigid and hydrophobic alkaloid. The conjugate with 2 cytisine and 16 glucoheptoamide substituents showed fast accumulation and no toxicity up to 200 µM concentration. The half-glucoheptoamidated PAMAM dendrimer was selected as a promising anticancer drug carrier for further applications.

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Section: Determination Of Conjugate Size By Dynamic Light Scattering supporting

confidence: 86%

Stepwise Glucoheptoamidation of Poly(Amidoamine) Dendrimer G3 to Tune Physicochemical Properties of the Potential Drug Carrier: In Vitro Tests for Cytisine Conjugates

et al. 2020

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“…The synthesis of PF127‐attached PAMAM dendrimer conjugates was carried out as previously described . The products were further analysed with H nuclear magnetic resonance (BrukerAVIII500M, Fällanden, Switzerland), Fourier transform infrared spectrometry (JASCO FT/IR‐4100, Tokyo, Japan) and element analysis (ThermoFinnigan Flash EA 1112, Milan, Italy).…”

Section: Methodsmentioning

confidence: 99%

Chemosensitization of doxorubicin in multidrug-resistant cells by unimolecular micelles via increased cellular accumulation and apoptosis

Wang

Han

Liu

et al. 2016

Journal of Pharmacy and Pharmacology

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“…The endothermic micellization process of pluronics with temperature rise is driven by a decrease in the polarity of ethylene oxide (EO) and propylene oxide (PO) segments and that results in the entropy gain in water, followed by orientations of monomers to form micelles (hydrophobic effect) [15]. Among various types of pluronics, pluronic F127 (PF127) has gained considerable attention due to its wide range of biomedical applications such as drug and gene delivery [166,167,168], tissue engineering [169,170] and burn wound covering [171]. The PPO segment of PF127 comprises of a hydrophobic core as a microenvironment for incorporation of lipophilic drugs whereas, PEO segment of PF127 prevents the adsorption and aggregation of loaded protein [172].…”

Section: Synthetic Polymersmentioning

confidence: 99%

Thermoresponsive Hydrogels and Their Biomedical Applications: Special Insight into Their Applications in Textile Based Transdermal Therapy

2018

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Various natural and synthetic polymers are capable of showing thermoresponsive properties and their hydrogels are finding a wide range of biomedical applications including drug delivery, tissue engineering and wound healing. Thermoresponsive hydrogels use temperature as external stimulus to show sol-gel transition and most of the thermoresponsive polymers can form hydrogels around body temperature. The availability of natural thermoresponsive polymers and multiple preparation methods of synthetic polymers, simple preparation method and high functionality of thermoresponsive hydrogels offer many advantages for developing drug delivery systems based on thermoresponsive hydrogels. In textile field applications of thermoresponsive hydrogels, textile based transdermal therapy is currently being applied using drug loaded thermoresponsive hydrogels. The current review focuses on the preparation, physico-chemical properties and various biomedical applications of thermoresponsive hydrogels based on natural and synthetic polymers and especially, their applications in developing functionalized textiles for transdermal therapies. Finally, future prospects of dual responsive (pH/temperature) hydrogels made by these polymers for textile based transdermal treatments are mentioned in this review.

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Preparation andin vitrocharacterization of pluronic-attached polyamidoamine dendrimers for drug delivery

Abstract: Findings from the in vitro study show that the PF127-attached PAMAM dendrimers may be potential carriers for drug delivery.

Cited by 27 publications

References 29 publications

Stepwise Glucoheptoamidation of Poly(Amidoamine) Dendrimer G3 to Tune Physicochemical Properties of the Potential Drug Carrier: In Vitro Tests for Cytisine Conjugates

Stepwise Glucoheptoamidation of Poly(Amidoamine) Dendrimer G3 to Tune Physicochemical Properties of the Potential Drug Carrier: In Vitro Tests for Cytisine Conjugates

Chemosensitization of doxorubicin in multidrug-resistant cells by unimolecular micelles via increased cellular accumulation and apoptosis

Thermoresponsive Hydrogels and Their Biomedical Applications: Special Insight into Their Applications in Textile Based Transdermal Therapy

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