Preparation and in vitro study of lipid nanoparticles encapsulating drug loaded montmorillonite for ocular delivery

Hou, Dongzhi; Hu, Sheng; Huang, Yi You; Gui, Ruyi; Zhang, Lanchun; Qi, Tao; Zhang, Chumei; Tian, Shuangyan; Komarneni, Sridhar; Ping, Qineng

doi:10.1016/j.clay.2015.10.028

Cited by 40 publications

(19 citation statements)

References 20 publications

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“…24 Nunes et al reported the loading and delivery of sertraline using Mt K10. 25 Our research has also shown that solid lipid nanoparticles 22 and chitosan nanoparticles 26 intercalated with Mt-drug nanocomposites to form nanocarriers can serve as promising ocular drugdelivery systems.…”

Section: Huang Et Almentioning

confidence: 99%

“…21,22 In recent years, Mt intercalated by drug molecules with different structures has attracted great interest from researchers, as the different structures have exhibited improved drug release properties. Zheng et al studied the intercalation of ibuprofen molecules into an Mt interlayer as a sustainedrelease drug carrier.…”

Section: Huang Et Almentioning

confidence: 99%

“…22,27,28 BH solution (100 mL 3 mg⋅mL −1 ) and 100 mg acid-Mt were mixed in a beaker. After the dispersion's pH had been adjusted to 4, the mixture was left static for adsorption to occur in a 50°C water bath for 6 hours.…”

Section: Preparation Of Mt-bh and Mt-bh-lpsmentioning

confidence: 99%

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A novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride

Huang¹,

Qi²,

Hou³

et al. 2017

IJN

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As a novel ion-exchange carrier with high surface area and excellent exchangeability, montmorillonite (Mt) was intercalated with betaxolol hydrochloride (BH) to form a nanocomposite and then encapsulated by liposomes (Mt-BH-LPs) for an ophthalmic drug-delivery system. The Mt-BH and Mt-BH-LPs were prepared by an acidification process and ethanol injection combined with ammonium sulfate gradient methods. The successful formation of Mt-BH and Mt-BH-LPs was verified by thermogravimetric analysis, X-ray diffraction, Fourier-transform infrared spectra, and transmission electron microscopy. Mt-BH-LPs possessed the favorable physical characteristics of encapsulation efficiency, drug loading, mean particle size, and ζ-potential. In vitro release studies indicated Mt-BH-LPs effectively maintained a relatively sustained slow release. Immortalized human corneal epithelial cell cytotoxicity, in vivo rabbit eye-irritation tests, and chorioallantoic membrane–trypan blue staining all revealed that Mt-BH-LPs had no obvious irritation on ocular tissues. A new in vitro tear-turnover model, including inserts containing human corneal epithelial cells, was designed to evaluate the precorneal retention time of Mt-BH-LPs. The results showed that Mt-BH-LPs maintained a certain BH concentration in tear fluid for a longer period than the BH solution. In vivo precorneal retention studies also indicated Mt-BH-LPs prolonged drug retention on the ocular surface more than the BH solution. Furthermore, pharmacodynamic studies showed that Mt-BH-LPs had a prolonged effect on decreasing intraocular optical pressure in rabbits. Our results demonstrated that Mt-BH-LPs have potential as an ophthalmic delivery system.

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Section: Huang Et Almentioning

confidence: 99%

Section: Huang Et Almentioning

confidence: 99%

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A novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride

Huang¹,

Qi²,

Hou³

et al. 2017

IJN

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“…1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan (MTT) and all components of buffer solutions were from Sigma-Aldrich Co (St Louis, MO, USA). All other chemical reagents used in the study were of high performance liquid chromatography (HPLC) or 24 After the exchange of interlayer metal ions with H + , Mt was ultrasonically treated for 5 minutes to obtain ultrafine montmorillonite treated with acid (acid-Mt) particles. Then, the acid-Mt was centrifuged and washed with deionized water until the pH was equal to 7.0.…”

Section: Materials and Methods Materialsmentioning

confidence: 99%

“…Previous results indicated that the release duration and burst release could be effectively improved by integrating Mt with polymers to form nanocomposites or microspheres. [21][22][23][24][25] Meanwhile, Mt is a 2:1 type swelling phyllosilicate with fine grain and large interlayer-planar spacing, which can allow cationic drug molecules intercalating into this space through ion-exchange method. It was reported that Mt intercalated with 5-fluorouracial and ibuprofen effectively improved the sustained release and loading capacity of drug molecules.…”

Section: Introductionmentioning

confidence: 99%

Controlled drug delivery for glaucoma therapy using montmorillonite/Eudragit microspheres as an ion-exchange carrier

Tian

et al. 2018

IJN

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Background Glaucoma is a serious eye disease that can lead to loss of vision. Unfortunately, effective treatments are limited by poor bioavailability of antiglaucoma medicine due to short residence time on the preocular surface. Materials and methods To solve this, we successfully prepared novel controlled-release ion-exchange microparticles to deliver betaxolol hydrochloride (BH). Montmorillonite/BH complex (Mt-BH) was prepared by acidification-intercalation, and this complex was encapsulated in microspheres (Mt-BH encapsulated microspheres [BMEMs]) by oil-in-oil emulsion–solvent evaporation method. The BH loaded into ion-exchange Mt was 47.45%±0.54%. After the encapsulation of Mt-BH into Eudragit microspheres, the encapsulation efficiency of BH into Eudragit microspheres was 94.35%±1.01% and BH loaded into Eudragit microspheres was 14.31%±0.47%. Results Both Fourier transform infrared spectra and X-ray diffraction patterns indicated that BH was successfully intercalated into acid-Mt to form Mt-BH and then Mt-BH was encapsulated into Eudragit microspheres to obtain BMEMs. Interestingly, in vitro release duration of the prepared BMEMs was extended to 12 hours, which is longer than both of the BH solution (2.5 hours) and the conventional BH microspheres (5 hours). Moreover, BMEM exhibited lower toxicity than that of BH solution as shown by the results of cytotoxicity tests, chorioallantoic membrane-trypan blue staining, and Draize rabbit eye test. In addition, both in vivo and in vitro preocular retention capacity study of BMEMs showed a prolonged retention time. The pharmacodynamics showed that BMEMs could extend the drug duration of action. Conclusion The developed BMEMs have the potential to be further applied as ocular drug delivery systems for the treatment of glaucoma.

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Antibactericidal nanoclay-based biomaterial for sustained delivery of tetracycline hydrochloride

et al. 2020

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Preparation and in vitro study of lipid nanoparticles encapsulating drug loaded montmorillonite for ocular delivery

Cited by 40 publications

References 20 publications

A novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride

A novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride

Controlled drug delivery for glaucoma therapy using montmorillonite/Eudragit microspheres as an ion-exchange carrier

Antibactericidal nanoclay-based biomaterial for sustained delivery of tetracycline hydrochloride

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