Preparation and physical-chemical characterization of benzylamide stationary phase for High Performance Liquid Chromatography

Abstract

This research project is focused on the development of an innovative methodology for the preparation benzylamide stationary phase, bonded onto silica surface. It involves the preparation of the stationary phase with benzoyl chloride and 3-aminopropyltrimethoxysilane and also the characterization by NMR-29Si , NMR-13C, IR and Elementary Analysis. The NMR-29Si spectrum of modified silica indicated the formation of siloxane bonding of the trialkoxysilane group with the silica, the IR spectra of stationary phase showed bands typical of aromatics and secondary amides. Analysing the NMR-13C spectra was possible to recognize all the carbons from the structure of the benzylamide stationary phase.