2016
DOI: 10.1016/j.jbiotec.2016.07.007
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Preparation and preliminary characterization of recombinant neurolysin for in vivo studies

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Cited by 22 publications
(26 citation statements)
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“…Table). These observations are complementing our published report indicating lack of effects on blood pressure, heart rate, body temperature, and glucose levels in mice treated with recombinant Nln (Wangler et al ). Notably, we observed higher activity of liver enzymes ALT and AST in mice treated with 50 mg/kg AgaA (Suppl.…”
Section: Discussionsupporting
confidence: 91%
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“…Table). These observations are complementing our published report indicating lack of effects on blood pressure, heart rate, body temperature, and glucose levels in mice treated with recombinant Nln (Wangler et al ). Notably, we observed higher activity of liver enzymes ALT and AST in mice treated with 50 mg/kg AgaA (Suppl.…”
Section: Discussionsupporting
confidence: 91%
“…Mouse primary cortical neurons and astroglial cells were isolated and cultured as described in our publications (Rashid et al ; Wangler et al ). Briefly, cerebral cortices were obtained from E16 embryos (CD‐1 mice, Charles River) and dissected in Hank’s balanced salt solution (HBSS) without Ca 2+ and Mg 2+ (product 14170161; Thermo Fisher Scientific, ) supplemented with 10 μg/mL gentamycin.…”
Section: Methodsmentioning
confidence: 99%
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“…This showed that the concentration of KBA was improved following IP treatment and proved to be an effective route of administration leading to increased KBA bioavailability. Several studies used different approaches to enhance boswellic acids bioavailability (Skarke et al, 2012; Du et al, 2015) and many have reported in animal models of disease the biological effects of drugs after IP administration indicating bioavailability of these large molecules administered by this route (Lee et al, 2011; Zhang et al, 2014; Wangler et al, 2016; Chang et al, 2018). IP route of administration have proven to be more effective because the rate and extent of drug absorption is faster in IP followed by intramuscular than the oral routes (Wolfensohn and Lloyd, 1994).…”
Section: Discussionmentioning
confidence: 99%
“…The lack of cytotoxicity, low blood stability, systemic bioavailability and pharmacokinetics of intraperitoneally administered recombinant neurolysin, and its effects on critical physiological parameters in mice have already been demonstrated [43]. These studies suggest that NEL can serve as a research tool and could contribute to a better comprehension of the functional significance of this peptidase in diseases [44,45]. The potential for the therapeutic application of THOP and NEL allied to the necessity of prolonged structural stability motivated the present study to develop THOP, and NEL capped GNPs.…”
Section: Introductionmentioning
confidence: 86%