Preparation and properties of a novel carbon nanotubes/poly(vinyl alcohol)/epidermal growth factor composite biological dressing

Abstract

Abstract. Wound dressings with drug delivery system have drawn increasing attention in skin damage recombination. Herein, a novel composite biological dressing was prepared and based on poly(vinyl alcohol) (PVA) combined with carbon nanotubes (CNTs) and epidermal growth factor (EGF) by electrospinning on gauze. The properties of the CNTs/PVA/EGF composite dressing were systemically investigated by general observation, and scanning electron microscopy (SEM). In vitro, the cytotoxicity of this dressing was investigated using a methyl thiazolyl tetrazolium (MTT) assay on L929 fibroblasts. In order to study the sustained release of EGF from this dressing, the concentration of EGF at different times was tested by ELISA. Furthermore, the biological activity of the released EGF was also evaluated using the MTT assay. Moreover, an in vivo experiment was conducted to observe whether this dressing was capable of improving healing in the model of wounded skin on rats. It was revealed that this dressing had a well-distributed microstructure by SEM. Additionally, the grade of cytotoxicity was low, and the EGF had a sustained release rate from this dressing. Furthermore, a maximum accumulative release rate of 12.47% was identified at 12 h, and was retained at 9.4% after 48 h. Simultaneously, the relative growth rate of L929 fibroblasts in the 12 h experimental group and 48 h group was 291.24 and 211.3%, respectively. Next, the efficacy of these products was evaluated in vivo using Sprague-Dawley rats with a skin injury model. The healing of wounded skin of rats was sped up by this dressing based on the gross and histological appearances. From 7 to 10 days, the wounds in the experimental group were almost healed. In conclusion, this CNTs/PVA/EGF dressing had a well-distributed structure and an ability to release EGF at a sustained rate with the activity being favorable. On the basis of those results, a positive influence of designed dressing for accelerated wound healing was confirmed.

Epidermal and fibroblast growth factors incorporated polyvinyl alcohol electrospun nanofibers as biological dressing scaffold

“…Amounts of 100 µg EGF and 100 µg FGF were dissolved in BSA/PBS in separate beakers. The growth factor solution mixed with the polymer solution at a temperature of 30 °C by applying a water bath concept for 30 min 13 , 31 . For all nanofibers were set at PVA:EGF:FGF, 9.5:0.5:0.5 v/v, respectively.…”

“…Tissue development or repair regulated by growth factors proteins along with several cellular processes within the extracellular matrix (ECM) 29 . Among the several families of the cytokines and growth factors 30 the epidermal growth factor (EGF) considered the first and foremost factor to promote wound healing 31 . It is mitogenic, form various epithelial cells, hepatocytes and fibroblasts 32 , 32 .…”

“…It possesses high-affinity receptors that are expressed by both fibroblasts and keratinocytes. EGF utilization is mainly to improve epidermal and mesenchymal regenerations, cell mobility, proliferation and ECM synthesis, which consequently facilitate wound healing 31 , 33 . At the same time, fibroblast growth factor (FGF) 32 Acidic and basic are the most known FGF classification 15 , 32 , 34 , 35 .…”

Effects of hydrogen peroxide on biological characteristics and osteoinductivity of decellularized and demineralized bone matrices

“…At each time point, cell proliferation in each group ( n = 6) was analyzed by the CCK‐8 kit (Dojindo, Japan) according to the manufacturer's instructions. For each group, the relative growth rate (RGR, %) of NIH‐3T3 fibroblasts was considered to be indicative of the degree of cytotoxicity …”

“…For each group, the relative growth rate (RGR, %) of NIH-3T3 fibroblasts was considered to be indicative of the degree of cytotoxicity. 25 IHC analysis. IHC was used to detect residual bioactive molecules of the two materials, with the following primary antibodies: rabbit anti-BMP-2 (Abbiotec), rabbit anti-VEGF (Abbiotec), and mouse anti-TGF-β1 (Thermo Scientific).…”