Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings

Abstract

As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings. Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing. Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application. In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking. The dressings had a porous composite structure, which allowed them to quickly absorb and store a large number of wound exudates. Skin-like tensile performance allowed the dressings to provide a better healing environment. Antibacterial assay against Escherichia coli and Staphylococcus aureus indicated that the addition of Mino significantly improved the antibacterial activity of the wound dressings. The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time. Cell culture assay showed that the dressings had excellent cell biocompatibility. As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds.

Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair

“…In recent years, biomaterial-based wound dressings have been widely used, such as chitosan (CS) [9], polymeric nanofibers [10], collagen protein [11], and sodium alginate [12], among which CS is a cationic marine natural polysaccharide obtained from chitin after deacetylation of the N-acetyl glucosamine groups [13]. It is extensively applied in clinical practice due to the advantages of being self-biodegradable, biocompatible, non-antigenic, water absorbable, air and moisture permeable, non-toxic, and well adherent to skin without irritation [14].…”

The seaweed holobiont: from microecology to biotechnological applications

“…Alginate hydrogels have gradually played an irreplaceable role in wound healing, drug delivery and tissue engineering applications because they maintain the structural similarity of the extracellular matrix in tissues and can effectively induce them to play a role in self‐healing (Abasalizadeh et al ., 2020 ; Aswathy et al ., 2020 ; Raus et al ., 2021 ; Sahoo and Biswal, 2021 ). Moreover, non‐wovens made from alginate fibres form soft hydrogels when in contact with body fluids, a property that is valuable in wound dressings, face masks, absorbent MATS and other healthcare textile materials (Gao and Xiangyu, 2018a , b ; Gao et al ., 2019 ).…”

Microscopic Techniques for the Analysis of Micro and Nanostructures of Biopolymers and Their Derivatives

“…In addition to providing the elemental composition and content, it helps in the analysis of dispersion and distribution of compounds within the sample. For example, EDX mapping can be helpful in determining the distribution of carboxymethyl chitosan and calcium alginate in a composite by analyzing the N element content of chitosan and Ca element of calcium alginate ( Figure 9) [152].…”

“…Energy-dispersive X-ray (EDX) mapping images of carboxymethyl chitosan and calcium alginate composite dressings[152]. (Reprinted with permission fromGao et al, 2019. ) …”