Preparation and rebinding properties of protein‐imprinted polysiloxane using mesoporous calcium silicate grafted non‐woven polypropylene as matrix

Abstract

Calcium silicate particle containing mesoporous SiO2 (CaSiO3@SiO2) was grafted on the surface of non-woven polypropylene. The PP non-woven grafted calcium silicate containing mesoporous SiO2 (PP-g-CaSiO3@SiO2) was used as the matrix to prepare bovine serum albumin (BSA) molecularly imprinted polysiloxane (MIP) by using silanes as the functional monomers and BSA as the template. PP non-woven grafted BSA-imprinted polysiloxane (PP-g-CaSiO3@SiO2 MIP) was characterized by scanning electron microscope (SEM), Fourier transform infrared spectometry (FTIR) and drilling string compensator (DSC). Influence factors on the rebinding capacity of the MIP were investigated, such as grafting degree, the pH in treating CaSiO3 and the type and proportion of silanes. The rebinding properties of BSA on PP-g-CaSiO3@SiO2 and MIP were investigated under different conditions. The results indicated that the rebinding capacity of MIP for BSA reached 56.32 mg/g, which was 2.65 times of NIP. The non-woven polypropylene grafted BSA-imprinted polysiloxane could recognize the template protein and the selectivity factor (β) was above 2.4 when using ovalbumin, hemoglobin and γ-globulin as control proteins. The PP-g-CaSiO3@SiO2 MIP has favorable reusability.

Improvement of Adsorption Selectivity of Ion-Exchange Chromatography through the Double-Recognition Mode

“…Besides, the IF template of BSA during pH 5.0 and 8.0 was from 2.26 to 2.38. Compared with the existing BSA-imprinted resins, such as hydrophilic protein-imprinted resin (IF 5.78), protein-imprinted polysiloxane (IF 2.65), imprinted chitosan nanoparticle (IF 2.35), and imprinted mesoporous calcium silicate (IF 2.25), the IFs of DEAE@BSA were not bad. The highest α was around 3.56 at pH 7.0, which indicates that pH 7.0 was a good operational condition for DEAE@BSA to separate BSA.…”

“…In addition, BSA structure maintained stable without undergoing any significant conformational changes 37 and matched these cavities well under pH conditions tested. It should be noted 38 proteinimprinted polysiloxane (IF 2.65), 39 imprinted chitosan nanoparticle (IF 2.35), 40 and imprinted mesoporous calcium silicate (IF 2.25), 41 the IFs of DEAE@BSA were not bad. The highest α was around 3.56 at pH 7.0, which indicates that pH 7.0 was a good operational condition for DEAE@BSA to separate BSA.…”

Molecularly Imprinted Membranes: Past, Present, and Future

“…Other types of composite MIMs are being built on the same concept of heterogeneous mixing of at least two different compounds, an organic one and an inorganic one. The organic phase is represented by the preorganized imprinting mixture, whereas the inorganic phase is represented by the supporting matrix on which the polymerization takes place, for example, microporous alumina (Al 2 O 3 ) membranes with 2–4-μm nominal pore size and 2-mm thickness , or silica (SiO 2 ). , In many other cases, the inorganic phase has been replaced by an organic phase, such as nylon, ,,− poly­(vinylidene fluoride) ,, (example of membrane characteristics: 0.22-μm pore size and 180-μm thickness), polypropylene, ,, regenerated cellulose , (e.g., 0.45-μm average pore diameter, 100-μm thickness), and cellulose acetate, ,,,, but the mixing of phases remains homogeneous up to micrometer dimensions and heterogeneous at submicronic dimensions, so these membranes are considered to be composites. Calcium alginate, , sodium alginate, , polyamide, , polycarbonate, polysulfone, , and polypyrrole have also been used as matrices for composite MIMs.…”

Protein molecularly imprinted polysiloxane prepared using calcium silicate/calcium alginate composite membrane as the matrix

“…分别将1片Hb印迹和非印迹膜放入10 mL浓度为 1.34 mg/mL的Hb水溶液中 [21] , 每隔一段时间取上清液 用紫外分光光度计测定其在278 nm波长处的吸光度, 根据标准曲线计算Hb浓度的变化, 参考文献 [15]计算 平衡吸附量Q e (mg/g), 画吸附动力学曲线.…”