Preparation and use of insolubilized amyloglucosidase for the production of sweet glucose liquors

Abstract

SummaryAmyloglucosidase (EC. 3.2.1.3), partially purified from an Aspergillus species, was chemically attached to DEAE cellulose using the bifunctional reagent 2-amino-4,6-dichloro-s-triazine. The action of the insolubilized enzyme derivative on dilute maltose and dextrin solutions was studied in a packed bed. A second and deeper bed was used to demonstrate the possibility of a continuous process for raising the dextrose equivalents of "glucose" liquors of high concentration formed by acid hydrolysis of maize starch.

Preparation and Properties of Paper Sheets Incorporating Immobilised Enzymes

“…Lilly and coworkers (Kayetal. 1968;Seif et al 1969;Sharp et al 1969;Wilson et al 1969) have prepared immobilised enzymes on filter paper and DEAE filter paper, the activities of which were maintained after storage for two years at 2-5°C (Lilly 1971). Broun and co-workers (Broun et al 1969(Broun et al , 1973 have studied the immobilisation of enzymes on cellophane.…”

Adsorption of Glucoamylase on DEAE‐Cellulose

“…Recent development of various techniques of immobilization of enzymes provides the possibility to utilize them in a cyclic or continuous process many times, thus lowering the costs and eventually simplifying the technological operations. GA was immobilized through covalent bonds to ceramic supports [ 11, porous glasses [2], DEAE-cellulose [3], bonding to neutral carriers through metal ions [S], entrapment in polyacrylamide gel [4]. A particulary suitable method for immobilizing the enzyme is its adsorption in view of an easy access of substrate and simplicity of the preparation procedure.…”

Production of Glucose From Tapioca (Cassava Starch) and Farinha De Mandioca (Cassava Meal)

“…The sources of these enzymes and their potential applications for industrial use have been reviewed (Underkofler and Danault, 1965;Sherwood, 1966;Wurzburg and Szymanski, 1970;Allen and Dawson, 1975). Several investigators have reported preparations of insolubilized amyloglucosidase and o-amylase and their use in the production of glucose syrups (Wilson and Lilly, 1969;Bachler et al, 1970;Park and Lima, 1973;Park, 1974;Tachauer et al, 1974;Linko et al, 1975). Others have described and compared the costs of batch and continuous process designs for the conversion of starch into dextrose and dextrose syrups by acid-enzyme and dual-enzyme processes (Sinclair, 1965;Kingma, 1966Kingma, , 1969Ewing and Harrington, 1967;Aschengreen, 1975).…”