Preparation, characterization and in vitro evaluation of calothrixin B liposomes

Yingyuad, Peerada; Sinthuvanich, Chomdao; Leepasert, Theerachart; Thongyoo, Panumart; Boonrungsiman, Suwimon

doi:10.1016/j.jddst.2018.02.010

Cited by 16 publications

(7 citation statements)

References 45 publications

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“…Nanoliposomes have been widely studied to know their interaction effects in different strains, cultures, and animal models for the development of new drugs, vaccines, improvement of photodynamic and cancer therapy, or even as a tool for the detection of several diseases. Among the current biomedical treatments, chemotherapy sensitization of glioblastoma (75 nm) (Papachristodoulou et al, 2019 ), gastrointestinal disorders (145 nm) (Chen et al, 2020 ), cutaneous (20 nm), and fungal infections (100 nm) (Saadat et al, 2016 ; Bhagat et al, 2019 ), encapsulation of calothrixin B as anticancer agent (108 nm) (Yingyuad et al, 2018 ) are included as some of the successful examples of nanoliposomes as drug delivery mechanisms. Figure 4 Illustrates drug administration and release pathways of nanoliposomes against cancer cells.…”

Section: Biomedical Applications Of Nanoliposomesmentioning

confidence: 99%

Insight Into Nanoliposomes as Smart Nanocarriers for Greening the Twenty-First Century Biomedical Settings

Aguilar-Pérez

Avilés-Castrillo

Medina

et al. 2020

Front. Bioeng. Biotechnol.

100

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The necessity to develop more efficient, biocompatible, patient compliance, and safer treatments in biomedical settings is receiving special attention using nanotechnology as a potential platform to design new drug delivery systems (DDS). Despite the broad range of nanocarrier systems in drug delivery, lack of biocompatibility, poor penetration, low entrapment efficiency, and toxicity are significant challenges that remain to address. Such practices are even more demanding when bioactive agents are intended to be loaded on a nanocarrier system, especially for topical treatment purposes. For the aforesaid reasons, the search for more efficient nano-vesicular systems, such as nanoliposomes, with a high biocompatibility index and controlled releases has increased considerably in the past few decades. Owing to the stratum corneum layer barrier of the skin, the in-practice conventional/conformist drug delivery methods are inefficient, and the effect of the administered therapeutic cues is limited. The current advancement at the nanoscale has transformed the drug delivery sector. Nanoliposomes, as robust nanocarriers, are becoming popular for biomedical applications because of safety, patient compliance, and quick action. Herein, we reviewed state-of-the-art nanoliposomes as a smart and sophisticated drug delivery approach. Following a brief introduction, the drug delivery mechanism of nanoliposomes is discussed with suitable examples for the treatment of numerous diseases with a brief emphasis on fungal infections. The latter half of the work is focused on the applied perspective and clinical translation of nanoliposomes. Furthermore, a detailed overview of clinical applications and future perspectives has been included in this review.

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Section: Biomedical Applications Of Nanoliposomesmentioning

confidence: 99%

Insight Into Nanoliposomes as Smart Nanocarriers for Greening the Twenty-First Century Biomedical Settings

Aguilar-Pérez

Avilés-Castrillo

Medina

et al. 2020

Front. Bioeng. Biotechnol.

100

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“…The obtained formulation is then freeze-dried, and the resulting powder is rehydrated when needed. Encapsulation efficiencies (EEs) with those methods can go up to 90% with lipophilic compounds, but is around 10–30% with hydrophilic drugs [ 9 , 14 ]. EE can be increased by the active drug loading technique.…”

Section: Methods Of Preparation Of Liposomal Therapeuticsmentioning

confidence: 99%

“…EE is defined as the percentage of drug successfully entrapped into liposomes with regards to the initial amount of drug used. EE mostly depends on phospholipids compositions, lipids to drug ratio, and the methods of preparation [ 9 ]. Various analytical methods like Ultraviolet-visible spectroscopy, high-pressure liquid chromatography, gas chromatography, gas chromatography-mass spectroscopy, and even quantitative NMR can be used to determine the concentration of drug inside the liposomes, depending on the nature of the active compound [ 27 , 47 ].…”

Section: Methods Of Characterizationmentioning

confidence: 99%

“…Liposomes are mostly synthesized with cholesterol and an array of phospholipids constituents, whose chemical properties ultimately dictate liposomal properties [ 9 ]. The most commonly used phospholipids are phosphatidylcholines (PC), phosphatidylethanolamine (PE), phosphatidylserines (PS), phosphatidylglycerol (PG), and sphingomyelin [ 10 ].…”

Section: Composition Of Liposomesmentioning

confidence: 99%

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Lipid-Based Drug Delivery Systems for Diseases Managements

Gbian

Omri

2022

Biomedicines

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Liposomes are tiny lipid-based vesicles composed of one or more lipid bilayers, which facilitate the encapsulation of hydrophilic, lipophilic, and amphiphilic biological active agents. The description of the physicochemical properties, formulation methods, characteristics, mechanisms of action, and large-scale manufacturing of liposomes as delivery systems are deeply discussed. The benefits, toxicity, and limitations of the use of liposomes in pharmacotherapeutics including in diagnostics, brain targeting, eye and cancer diseases, and in infections are provided. The experimental approaches that may reduce, or even bypass, the use of liposomal drug drawbacks is described. The application of liposomes in the treatment of numerous diseases is discussed.

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“…Nanoparticles, such as mesoporous silica nanoparticles (MSNs), carbon nanotubes, liposomes, fluorescent quantum dots, gold nanorods, superparamagnetic nanoparticles, etc., are widely used in biosensing, bioimaging, and cancer therapy . Studying the behaviors of nanoparticles in the cellular world can provide important information for optimizing their biomedical performance. − To date, research on nanoparticles within cells are usually carried out either by conventional fluorescence microscopy or by electron microscopy. − Fluorescence microscopy can observe specific cell components or cellular events, and it has been widely used in noninvasive and real-time bioimaging. − In spite of such advantages, fluorescence microscopy cannot reveal detailed structural organization of biomolecules or subcellular organelles because the diffraction of light limits the spatial resolution of conventional fluorescence microscopy to about 200–300 nm in the lateral direction and 500–700 nm in the axial direction . As for electron microscopy, the resolution can reach nanometers, but the disadvantages such as invasiveness, complicated sample preparation, and expensive instrumentation significantly limit its biomedical application. , Therefore, there is still an urgent need to develop an easy-to-perform method with high spatial resolution to study the behaviors of nanoparticles in the cellular world.…”

mentioning

confidence: 99%

Direct Observation of Nanoparticles within Cells at Subcellular Levels by Super-Resolution Fluorescence Imaging

et al. 2019

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Direct observation of nanoparticles with high spatial resolution at subcellular levels is of great importance to understand the nanotoxicology and promote the biomedical applications of nanoparticles. Super-resolution fluorescence microscopy can break the diffraction resolution limit to achieve spatial resolution of tens of nanometers, making it ideal for highly accurate observation of nanoparticles in the cellular world. In this study, we introduced the employment of super-resolution fluorescence imaging for monitoring nanoparticles within cells. Carbocyanine dyes Alexa Flour 647 labeled mesoporous silica nanoparticles (designated as MSNs-AF647) were constructed as the super-resolution imaging nanoplatform in this work as proof of concept. The MSNs-AF647 were incubated with Hela cells, and the nanoparticles within cells were further monitored by super-resolution fluorescence microscopy. The fluorescence images of MSNs-AF647 within cells captured with the super-resolution fluorescence microscopy showed a much higher spatial resolution than that obtained using conventional fluorescence microscopy, showing that super-resolution fluorescence images can provide more accurate information to locate the nanoparticles at the subcellular levels. Moreover, other functional molecules can be easily loaded into the MSNs-AF647 super-resolution imaging nanoplatform, which suggested that super-resolution fluorescence imaging can further be applied to various bioimaging-related areas, such as imaging-guided therapy, with the aid of the MSNs-AF647 nanoplatform. This study demonstrates that super-resolution fluorescence microscopy offers a highly accurate method to study nanoparticles in the cellular world. We anticipate this strategy may further be applied to research areas such as studying the nanotoxicology and optimization of nanoparticle-based bioprobes or drugs by designing new nanostructured materials with multifunctional properties based on MSNs-AF647.

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Preparation, characterization and in vitro evaluation of calothrixin B liposomes

Cited by 16 publications

References 45 publications

Insight Into Nanoliposomes as Smart Nanocarriers for Greening the Twenty-First Century Biomedical Settings

Insight Into Nanoliposomes as Smart Nanocarriers for Greening the Twenty-First Century Biomedical Settings

Lipid-Based Drug Delivery Systems for Diseases Managements

Direct Observation of Nanoparticles within Cells at Subcellular Levels by Super-Resolution Fluorescence Imaging

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