Preparation, characterization and in vivo evaluation of a combination delivery system based on hyaluronic acid/jeffamine hydrogel loaded with PHBV/PLGA blend nanoparticles for prolonged delivery of Teriparatide

Javan, Nika Bahari; Montazeri, Hamed; Shirmard, Leila Rezaie; Omid, Nersi Jafary; Barbari, Ghullam Reza; Amini, Mohsen; Ghahremani, Mohammad Hossein; Rafiee-Tehrani, Morteza; Dorkoosh, Farid Abedin

doi:10.1016/j.ejps.2017.02.018

Cited by 23 publications

(6 citation statements)

References 77 publications

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“…Therefore, characteristics like surface modifications or adjustment of physicochemical properties, and the biodegradation rate of the material are crucial. Several protocols for the preparation and modification of micro- and nano-scale PLGA systems have been established [6], [7], [8], [9], [10]. Depending on the manufacturing method and the operating parameters, the yield of the method and the loading efficiency of nano- and microparticles can vary drastically, influencing the therapeutic use [11], [12].…”

Section: Introductionmentioning

confidence: 99%

Development of a fast and precise method for simultaneous quantification of the PLGA monomers lactic and glycolic acid by HPLC

Pourasghar

Koenneke

Meiers

et al. 2019

Journal of Pharmaceutical Analysis

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Poly(lactide- co -glycolide acid) (PLGA) is an extraordinary well-described polymer and has excellent pharmaceutical properties like high biocompatibility and good biodegradability. Hence, it is one of the most used materials for drug delivery and biomedical systems, also being present in several US Food and Drug Administration-approved carrier systems and therapeutic devices. For both applications, the quantification of the polymer is inalienable. During the development of a production process, parameters like yield or loading efficacy are essential to be determined. Although PLGA is a well-defined biomaterial, it still lacks a sensitive and convenient quantification approach for PLGA-based systems. Thus, we present a novel method for the fast and precise quantification of PLGA by RP-HPLC. The polymer is hydrolyzed into its monomers, glycolic acid and lactic acid. Afterwards, the monomers are derivatized with the absorption-enhancing molecule 2,4′-dibromoacetophenone. Furthermore, the wavelength of the derivatized monomers is shifted to higher wavelengths, where the used solvents show a lower absorption, increasing the sensitivity and detectability. The developed method has a detection limit of 0.1 µg/mL, enabling the quantification of low amounts of PLGA. By quantifying both monomers separately, information about the PLGA monomer ratio can be also directly obtained, being relevant for degradation behavior. Compared to existing approaches, like gravimetric or nuclear magnetic resonance measurements, which are tedious or expensive, the developed method is fast, ideal for routine screening, and it is selective since no stabilizer or excipient is interfering. Due to the high sensitivity and rapidity of the method, it is suitable for both laboratory and industrial uses.

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Section: Introductionmentioning

confidence: 99%

Development of a fast and precise method for simultaneous quantification of the PLGA monomers lactic and glycolic acid by HPLC

Pourasghar

Koenneke

Meiers

et al. 2019

Journal of Pharmaceutical Analysis

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“…Similarly, Montanheiro et al 25 reported on the PHBV scaffolds of low porosity produced by the addition of cellulose nanocrystals. Javan et al 31 also observed lower swelling degree of hydrogel due to the addition of PHVB/PLGA NPs into the polymeric matrix and they attributed this observation to the restricted movement and relaxation of the polymeric structure.…”

Section: Discussionmentioning

confidence: 97%

Chitosan hydrogel covalently crosslinked by gold nanoparticle: Eliminating the use of toxic crosslinkers

Tenório

Montanheiro

Santos

et al. 2020

J of Applied Polymer Sci

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Tissue engineering has directed a lot of effort toward the development of devices with suitable biocompatibility and mechanical properties. Chitosan has been pointed as a valuable material to be applied in scaffolds due to its antimicrobial activity and biocompatibility. Nevertheless, the low mechanical resistance associated with the requirement of toxic crosslinkers has hampered translational application of chitosan hydrogel. Herein, the use of gold nanoparticles (AuNP) as crosslinker is reported as a great strategy to obtain chitosan hydrogel without using toxic reactants. In addition, the resultant chitosan hydrogel, crosslinked by AuNP of 30 nm (AuNP30), presented outstanding properties compared to chitosan hydrogel crosslinked by glutaraldehyde. Chitosan hydrogel crosslinked by AuNP30 presented lower porosity, which provided lower swelling degree and slower degradation rate. In addition, compressive strength was about two times higher than the chitosan hydrogel crosslinked by glutaraldehyde. The crosslink by AuNP30 also increased the biocompatibility of the hydrogel. Chitosan hydrogel crosslinked by AuNP30 did not show cytotoxicity against MEF cells, whereas cell viability of cells incubated with extract from chitosan hydrogel crosslinked by glutaraldehyde was only 41%. In conclusion, the results reported herein pointed that the use of AuNP30 as crosslinker agent provided to chitosan hydrogel enhanced properties that made it suitable to application in biomedical devices.

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“…A carbodiimide compound such as 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) is a widely used activating agent for cross-linking HA, which is often employed to establish stable covalent bonds between carboxyl groups found in biopolymers and primary amines. To initiate the reaction, the biomolecules intended for cross-linking are dissolved in a buffer solution with a slightly acidic pH (around pH 5-6) [49,50]. The reaction mixture is then supplemented with EDC, which reacts with the carboxyl groups in the biopolymers to form an activated intermediate.…”

Section: Carbodiimide Cross-linkingmentioning

confidence: 99%

Exploring the Progress of Hyaluronic Acid Hydrogels: Synthesis, Characteristics, and Wide-Ranging Applications

Gholamali,

Vu,

et al. 2024

Materials

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This comprehensive review delves into the world of hyaluronic acid (HA) hydrogels, exploring their creation, characteristics, research methodologies, and uses. HA hydrogels stand out among natural polysaccharides due to their distinct features. Their exceptional biocompatibility makes them a top choice for diverse biomedical purposes, with a great ability to coexist harmoniously with living cells and tissues. Furthermore, their biodegradability permits their gradual breakdown by bodily enzymes, enabling the creation of temporary frameworks for tissue engineering endeavors. Additionally, since HA is a vital component of the extracellular matrix (ECM) in numerous tissues, HA hydrogels can replicate the ECM’s structure and functions. This mimicry is pivotal in tissue engineering applications by providing an ideal setting for cellular growth and maturation. Various cross-linking techniques like chemical, physical, enzymatic, and hybrid methods impact the mechanical strength, swelling capacity, and degradation speed of the hydrogels. Assessment tools such as rheological analysis, electron microscopy, spectroscopy, swelling tests, and degradation studies are employed to examine their attributes. HA-based hydrogels feature prominently in tissue engineering, drug distribution, wound recovery, ophthalmology, and cartilage mending. Crafting HA hydrogels enables the production of biomaterials with sought-after qualities, offering avenues for advancements in the realm of biomedicine.

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Preparation, characterization and in vivo evaluation of a combination delivery system based on hyaluronic acid/jeffamine hydrogel loaded with PHBV/PLGA blend nanoparticles for prolonged delivery of Teriparatide

Cited by 23 publications

References 77 publications

Development of a fast and precise method for simultaneous quantification of the PLGA monomers lactic and glycolic acid by HPLC

Development of a fast and precise method for simultaneous quantification of the PLGA monomers lactic and glycolic acid by HPLC

Chitosan hydrogel covalently crosslinked by gold nanoparticle: Eliminating the use of toxic crosslinkers

Exploring the Progress of Hyaluronic Acid Hydrogels: Synthesis, Characteristics, and Wide-Ranging Applications

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