Preparation, characterization and wound healing effect of alginate/chitosan microcapsules loaded with polysaccharides from Nostoc Commune Vaucher

Abstract

Biologically active coating materials could promote the growth of granulation tissue as auxiliary materials, while natural polysaccharides could promote vascular regeneration and wound healing. Therefore, in this study, ultrasound-assisted extract of Nostoc commune Vaucher polysaccharides (UAP) yield after the process optimization was 12.89 ± 0.24%, which was used to prepare microcapsules by emulsification and cross-linking. The effect of alginate/chitosan-UAP composite materials on wound healing in an experimental rat model for 14 d and its physical properties were evaluated. In vitro experiments indicated that the UAP microcapsule material had a porous and loose three-dimensional network structure, and had good biocompatibility and swelling properties as a wound healing material. Animal experiments indicated that UAP microcapsules could extremely significantly promote wound healing (P < 0.01), and wound closure rate reached 79.16 ± 3.91% on 14th day. Meanwhile UAP microcapsules might promote angiogenesis and granulation growth by enhancing immunity and increasing the expression of VEGF and miR-21. Therefore, the composites of UAP microcapsules have shown encouraging results as a potential dressing for wound healing.

Modulation of immune cum inflammatory pathway by earthworm granulation tissue extract in wound healing of diabetic rabbit model

“…Conversely, free radical mediated cellular damage marker (LPO) were significantly reduced in EGTE groups, apparently significant reduction of LPO levels is an important indication of free radical scavenging activity of EGTE, which ameliorates oxidative damage and henceforth modulates inflammation which plays an important role in wound healing. There are some earlier studies that have reported free radical scavenging activity of crude earthworm tissue extracts, earthworm coelomic fluid extracts and other preparations derived from earthworms, hence based on results of present study it can be postulated that EGTE provides protection to cells and cellular macromolecules under oxidative stress conditions [ 51 ]. Researchers have found that free radicals in tissue micro-environment are involved in delayed wound healing in diabetic patients, hence amelioration of oxidative stress by EGTE protect cells from pathological changes initiated by free radicals [ 52 ].…”

The protective effect of Nostoc commune Vauch. polysaccharide on alcohol‐induced acute alcoholic liver disease and gut microbiota disturbance in mice

“…has the anticancer activity, 14 hypoglycemic effect, 15 and wound healing ability. 16 Our previous study also had found that N. commune Vauch. polysaccharide (NCVP) is beneficial to maintain the gut health.…”

“…Polysaccharide as the main components of N. commune Vauch. has the anticancer activity, 14 hypoglycemic effect, 15 and wound healing ability 16 . Our previous study also had found that N. commune Vauch.…”

Extraction kinetic model of polysaccharide from Codonopsis pilosula and the application of polysaccharide in wound healing

“…The dispersion and microstructure of the microcapsules were observed by TEM (TEM-1200EX, JEOL Corporation, Japan) [38,39] 2.5.9. Mechanical property test of CPNP microcapsules According to the reference outlined method [40].…”