Preparation d'invertase insolubilisée par fixation sur bentonite

Monsan, Pierre; Durand, Gilbert

doi:10.1016/0014-5793(71)80679-8

Cited by 23 publications

(5 citation statements)

References 12 publications

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“…The immobilization of a-chymotrypsin [86,87], peroxidase [88,89], invertase [90,91], lipase [92,93], and tyrosinase [94,95] on clay has been intensively studied. Layered phyllosilicates display a wide range of physico-chemical properties depending on their chemical composition and the type of isomorphous metal cation substitution in the tetraedral and octaedral layers and interlayers.…”

Section: Immobilization In Clay Minerals and Related Materialsmentioning

confidence: 99%

“…In order to fix the enzyme irreversibly at the surface of the clay layers different processes have been tried. In order to fix invertase on bentonite, Monsan and Durand [90] previously treated the clay mineral with a coupling agent, namely cyanuryl chloride, but the amount of invertase immobilized was poor even though a significant percentage (13.4 %) of catalytic activity was retained after anchoring. However, as for other host structures, increase in stability against denaturing factors (pH, temperature) was observed.…”

Section: Immobilization In Clay Minerals and Related Materialsmentioning

confidence: 99%

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Enzyme‐Based Bioinorganic Materials

Forano

Prévot

2007

Bio‐inorganic Hybrid Nanomaterials

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Trends in research materials currently focus on innovation in the field of intelligent or smart materials [1]. Smart materials are materials that display functionalities able to detect a change in the near environment, answer to a stimulus, modify a property, store, transfer or convert a signal, in a sense behave like living cells. The design of smart materials focuses on the concept of biomimetic materials [2][3][4] in order to reproduce specific and selective properties not deliverable by standard chemical or physical processes. Hence, there has been much effort concentrated on the development of enzyme-based biohybrid materials. Enzyme-based biohybrid materials are under much investigation for the operation of physical or chemical functions. Enzymes display active and selective properties such as molecular recognition, complexation or carrier functions, and a wide range of catalytic reactions (hydrolysis, condensation, redox reaction, isomerization, reaction transfer). Enzymes are thus very attractive as an alternative to traditional catalysts when chemical routes are difficult to apply. Enzymes are able to catalyze regio-and stereo-selective reactions with rate accelerations up to 10-17 fold [17]. They are interesting materials for realization of sensing devices, recognition systems and nanoreactors with many potential industrial developments in biocatalysis [4] for the food industry or effluent treatment [5,6], in biomedical domains for medical implants or enzyme delivery [7], in bioanalysis and affinity chromatography [8,9], and in biosensor technology [10][11][12].Engineering the development of biohybrid enzyme-based materials requires solution of the limitations of the use of enzymes due to their intrinsic fragility against the various environmental stresses and their narrow range of working conditions (e.g. short pH and temperature range). Embedding enzymes in protective matrices in order to solve these problems has been envisaged for many years [13,14]. Immobilization of enzymes in solid supports is seen as a strategy to preserve the active site, to improve the performance of the enzyme, that is, its activity, reaction rate and long-term stability, and to allow enzyme reuse. Physical and chemical properties of the solid supports such as particle size, surface area, pore diameter, mechanical j443 Bio-inorganic Hybrid Nanomaterials. Edited

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Section: Immobilization In Clay Minerals and Related Materialsmentioning

confidence: 99%

Section: Immobilization In Clay Minerals and Related Materialsmentioning

confidence: 99%

Enzyme‐Based Bioinorganic Materials

Forano

Prévot

2007

Bio‐inorganic Hybrid Nanomaterials

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“…Most authors believe that some kind of chemical bond is formed in this case, or in other words, that an organic compound is graft-polymerized on an inorganic compound [l, 21. While the number of suitable inorganic compounds is rather limited, there is a large variety of organic materials that may be used for coating. Silica gel [l], zeolites [l], bentonite [3], metal oxides [l, 41 or glass [5,61 were used as the inorganic support. The surfaces of these compounds are rather inactive, but they can be chemically activated.…”

Section: Introductionmentioning

confidence: 99%

Reactive polymers. XXII. Preparation of poly(2,3‐epoxypropyl methacrylate) on porous glass

Kálal

Tlusťáková

1979

Acta Polymerica

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2.3-Epoxypropyl methacrylate sorbed on porous glass was polymcrized by radical mechanism. The polymerization conditions were chosen so that the polymer obtained was linear or only very slightly crosslinked. The amount of the polymer on glass can be varied arbitrarily by diluting the polymerization mixture or by varying the amount of water on glass. The specific surface of the system decreases with increasing polymer content. With increasing specific surface of porous glass, i. e. with decreasing diameter of the pores, the amount of the polymer bound on glass also increases. Reaktive Polymere X X I I . Herstellung von Poly(2,3-epoxypropylmethucrylat) auf poriisem GlasAn porosem Glas sorbiertes 2,3-Epoxypropylmethacrylat wurde radikalisch polymerisiert. Die Polymerisationsbedingungen wurden so gewahlt, daR das erhaltenc Polymer linear bzw. nur sehr schwach vernetzt war. Die Polymermenge auf dem Glas kann durch Verdiiunen der Polymcrisationsmischung oder durch Variation der auf dem Glas befindlichen Wassermenge variiert werden. Die spezifisehe Oberflache des Systems nimmt mit steigendem Polymergehalt ab. Mit zunelimender Oberflache des porosen Glases, d. h. abnehmendem Durchmesser der Poren, steigt die Menge des an das Glas gebundenen Polymers ebenfalls an. Peakyuonnocnoco6nbie nonu&epbi. X X I I . Cunmes n o~u ( 2 , . 3 -~n o~c u n p o n u n~e m a~p u~a m a )nu nopucmoa cmeme M e T O g O M P a R H H a J I b H O a llOJIHMCpH3aqHH COp6HpOBaHHOl'O H a IlOpHCTOM CTCIFJIC 2,3-aIlOIFCHIlpOIIHJIMCTaKpMnaTa 6 6 1~

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“…Some examples are the binding of an enzyme to cyanogen bromideactivated Sepharose (2) or intermolecular crosslinking of enzyme molecules with glutaraldehyde (14). Physical methods, which do not involve covalent bond formation, include adsorption of the enzyme to an insoluble support (11), entrapment within a gel (12) or microcapsule (3), and containment by semipermeable membranes (10). Various advantages and disadvantages can be ascribed to each method (21).…”

mentioning

confidence: 99%

“…Use of aluminum silicate clays to adsorb an enzyme was first reported in 1956 (9). Subsequently, several enzymes have been immobilized by this method (11,16,18), and more recently bentonite-immobilized penicillin amidase has been used for the commercial preparation of 6-aminopenicillanic acid (19). The enzyme cephalosporin acetylesterase catalyzes the hydrolysis of acetate from the 3'-position of cephalosporins (Fig.…”

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confidence: 99%

Preparation and Properties of a Cephalosporin Acetylesterase Adsorbed onto Bentonite

Abbott

Fukuda

1975

Antimicrob Agents Chemother

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A cephalosporin acetylesterase produced by Bacillus subtilis was immobilized by adsorption onto bentonite. The immobilized enzyme (E I ) and the soluble enzyme (E S ) exhibited Michaelis-Menton kinetics with 7-aminocephalosporanic acid (7-ACA): K m = 2.8 × 10 −3 M and K m = 3.2 × 10 −3 M, respectively. Similar kinetics were observed with 7-(thiophene-2-acetamido)cephalosporanic acid (cephalothin), but the K m value measured with E I (3.7 × 10 −3 M) was less than one-half that measured with this substrate and E S . The reduction in K m value was correlated with the ability of bentonite to adsorb cephalothin. The reaction products, acetate and deacetyl-7-ACA, were weak competitive inhibitors of E S and E I . The K i values for E I were 5.0 × 10 −2 M for acetate and 3.6 × 10 −2 M for deacetyl-7-ACA. Similar values were measured with E S and these substrates. E I retained about 80% of its initial activity after 3 weeks of storage in solution at 25 C. However, the enzyme dissociated from the bentonite particles during the deacetylation reaction. This dissociation was minimized by cross-linking E I with glutaraldehyde or bis-dimethyladipimidate, or by adding Al(OH) 3 to the suspension. With the latter addition, E I was stabilized so that it could be reused nine times before one-half of the initial activity was lost.

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Preparation d'invertase insolubilisée par fixation sur bentonite

Cited by 23 publications

References 12 publications

Enzyme‐Based Bioinorganic Materials

Enzyme‐Based Bioinorganic Materials

Reactive polymers. XXII. Preparation of poly(2,3‐epoxypropyl methacrylate) on porous glass

Preparation and Properties of a Cephalosporin Acetylesterase Adsorbed onto Bentonite

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