Préparation des Antigenes des Mycoplasmes (MLO) Pathogènes de la Flavescence Dorée, à Partir de Tissus Végétaux

Caudwell, A.; Kuszala, Catherine; Fleury, Agnès

doi:10.1111/j.1439-0434.1988.tb04459.x

Cited by 17 publications

(15 citation statements)

References 7 publications

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“…MLO-enriched extracts were prepared as previously described from FD-infected broadbean (Caudwell, Kuszala & Fleury, 1988), or from FD-infected leafhopper salivary glands. Healthy broadbeans and salivary glands were used as controls.…”

Section: Mlo-enriched Extractsmentioning

confidence: 99%

Attachment of the Flavescence doree pathogen (MLO) to leafhopper vectors and other insects

Lefol¹,

Caudwell²,

Lherminier³

et al. 1993

Annals of Applied Biology

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Flavescence dorCe (FD) is an important yellows disease of grapevine, caused by mycoplasma-like-organism (MLO) and is transmitted in the field by the leafhopper Scaphoideus titanus Ball. It can be transmitted in the laboratory between Vicia faba test plants by the leafhopper, Euscelidius variegatus Kbm. A technique to identify a specific attachment system between the MLO and the leafhopper vectors was developed. In this method, called "Double Dot", extracts of macerated healthy whole insects or organs applied to a support membrane or cryosections of healthy whole leafhoppers, are incubated with a MLO-enriched extract from FD-infected V. faba or FD-infected E. variegatus. Attached MLO cells were identified by immunolabelling using FD-MLO specific monoclonal antibodies. Attachment of MLO cells was obtained on extracts of healthy S. titanus and E. variegatus and on tissues such as salivary glands, hemolymph and alimentary tract. On cryosections, MLO attachment was obtained on acini IV and V of the salivary glands and on some acini 111, on the ventriculus of the alimentary tract, and on the abdomen fat bodies. "Double dot" experiments were done using other insect species, and MLO cells attachment was obtained on most MLO-vector insects but also on insects from a few non-vector species.

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Section: Mlo-enriched Extractsmentioning

confidence: 99%

Attachment of the Flavescence doree pathogen (MLO) to leafhopper vectors and other insects

Lefol¹,

Caudwell²,

Lherminier³

et al. 1993

Annals of Applied Biology

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“…In Portugal, FDp was first detected in the Portuguese DOC region of 'Vinhos Verdes' in 2006(deSousa et al, 2010. The visible external symptoms of grapevines infected by the flavescence dorée phytoplasma are similar to other grapevine yellows (GY), usually emerging after one to three years of latency after infection (Caudwell, 1964;Dermastia et al, 2017). Different studies have reported harmful effects of the infection at both physiological and molecular levels, revealing modifications in the primary and secondary metabolism.…”

Section: Introductionmentioning

confidence: 99%

The grapevine metabolite profile of phloem sap is modified by flavescence dorée

et al. 2023

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Flavescence dorée (FD) has been recorded in French vineyards since the mid-1950s; it has rapidly become a major threat to vineyard survival in different European grape-growing areas. Infection by the phytoplasma ‘Candidatus Phytoplasma vitis’ causes leaf chlorosis and disturbs primary and secondary core metabolic pathways; it can also occlude sieve plates, therefore affecting normal solute transport through the phloem. Thus, in the present study we hypothesised that the metabolomic fingerprint of the phloem sap changes in FDp-infected vines. The metabolome of the phloem sap collected by centrifugation from the bark of the stem of healthy and infected vines (cv. ‘Loureiro’) was profiled by ESI-HRMS at two snapshot time points: E-L 33 (grape berry still hard and green) and E-L 38 (grape berry at maturity). An untargeted analysis of the phloem sap enabled the identification of 476 metabolites, whereas the targeted analysis focused on eight main classes, namely acids, sugar and polyols, amino acids, phenolic acids, hormones, vitamins and N compounds, as well as on several minor classes like fatty acids, phenols or diols. Depending on the developmental stage of the vine, major differences were observed in the composition of cv. ‘Loureiro’ phloem sap in response to FD infection in terms of sugar, organic acid and amino acid content; furthermore, compounds involved in plant defense, such as salicylic acid, ABA, vitamin C and gallic acid also significantly changed. Overall, these results have contributed towards increasing knowledge about the complex interactions between vine and FD phytoplasma.

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“…Aquadulce) (Caudwell et al, 1970) using Scaphoideus titanus leafhoppers collected in FD-affected vineyards in the south-west of France in 1970 and 1992, respectively. FD92 has been maintained since this time by uninterrupted serial transmission from broad bean to broad bean using Euscelidius variegatus as an alternative leafhopper vector (Caudwell et al, 1972). FD70 and FD92 were also transmitted by infected E. variegatus to periwinkle (Catharantus roseus) and maintained by grafting (Caudwell et al, 1973).…”

Section: Methodsmentioning

confidence: 99%

A chromosome map of the Flavescence dorée phytoplasma

et al. 2008

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The Flavescence doré e phytoplasma (FD-P), a non-cultivable, plant-pathogenic bacterium of the class Mollicutes, is the causal agent of a quarantine disease affecting vineyards of southern Europe, mainly in southern France and northern Italy. To investigate FD-P diversity and phytoplasma genetic determinants governing the FD-P life cycle, a genome project has been initiated. A physical map of the chromosome of FD-P strain FD92, purified from infected broad beans, was constructed by performing restriction digests of the chromosome and resolving the fragments by PFGE. Single and double digestions of the chromosome with the enzymes SalI, BssHII, MluI and EagI were performed and used to map 13 restriction sites on the FD-P chromosome. The size of the chromosome was calculated to be 671 kbp. Southern blot analyses using cloned phytoplasma probes were carried out to assist in the arrangement of contiguous restriction fragments and to map eight genetic loci, including the two rRNA operons, the tuf, uvrB-degV and secY-map (FD9) genes, the FD2 marker and two orphan sequences (FDDH29 and FDSH05) isolated through subtractive suppression hybridization.

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Préparation des Antigenes des Mycoplasmes (MLO) Pathogènes de la Flavescence Dorée, à Partir de Tissus Végétaux

Cited by 17 publications

References 7 publications

Attachment of the Flavescence doree pathogen (MLO) to leafhopper vectors and other insects

Attachment of the Flavescence doree pathogen (MLO) to leafhopper vectors and other insects

The grapevine metabolite profile of phloem sap is modified by flavescence dorée

A chromosome map of the Flavescence dorée phytoplasma

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