Preparation, identification, and activity evaluation of antioxidant peptides from protein hydrolysate of corn germ meal

Abstract

Information on antioxidant peptides derived from corn germ meal (CGM) is seldom reported. In this paper, three novel antioxidant peptides including Met–Gly–Gly–Asn (MGGN; 377.42 Da), Met–Asn–Asn (MNN; 377.42 Da), and Met–Glu–Asn (MEN; 392.43 Da) were purified from the most active fractions of corn germ meal hydrolysate, and their activity was evaluated by radical scavenging, oxygen radical absorbance capacity, cellular antioxidant activity (CAA), and intracellular reactive oxygen species scavenging assays. MNN had a higher value for the CAA assay (1,213.79 μmol of QE/100 mmol) and could be most effective in preventing conditions favorable to intracellular oxidative stress. Finally, electron paramagnetic resonance spectroscopy directly verified the above experimental results by measuring the number of •OH trapped in the system. Thus, CGM could be considered as a potential source of antioxidant peptide and MNN might be regarded as a natural antioxidant. Practical applications CGM is a by‐product of corn industrial that includes ground corn germ and other parts of the corn kernel. In the past, some of them were discarded by factories in China. In this study, three novel antioxidant peptides were isolated from the hydrolysate of CGM, which greatly increased its economic value. In addition, these peptides have huge potential to serve as antioxidants in pharmaceutical, cosmetic, and functional food industries.

Physico‐chemical properties, antioxidant activity, and ACE inhibitory activity of protein hydrolysates from wild jujube seed

“…Xie et al (2019) reported that different molecular fractions from mung bean protein hydrolysates (MBPHs) exhibited different antioxidant ability and ACE inhibitory activity, where MBPHs-I (<3,000 Da) exhibited the strongest DPPH-and hydroxyl radicals-scavenging activity. Zhang et al (2019) also found that low molecular fractions (<1,000 Da) from protein hydrolysate of corn germ meal showed higher DPPH-and ABTS + radicalsscavenging activity than other fractions (1,000−3,000 Da and 3,000−10,000 Da). Previous reports indicated that low molecular weight fractions from protein hydrolysates exhibited stronger antioxidant ability than high molecular weight protein and hydrolysates (Zheng et al, 2018).…”

“…Zhang et al. (2019) also found that low molecular fractions (<1,000 Da) from protein hydrolysate of corn germ meal showed higher DPPH‐ and ABTS + radicals‐scavenging activity than other fractions (1,000−3,000 Da and 3,000−10,000 Da). Previous reports indicated that low molecular weight fractions from protein hydrolysates exhibited stronger antioxidant ability than high molecular weight protein and hydrolysates (Zheng et al., 2018).…”

Plant Protein-Derived Active Peptides: A Comprehensive Review

“…The research of corn active peptides mainly focuses on antioxidant peptides, which have been isolated from corn gluten meal, corn germ, and corn silk. ,− In contrast, rice active peptides have a wide range of research. Tong found that the rice-derived peptide AAGALPS (25 μg/mL) attenuated TNF-α-induced injury by restraining inflammatory responses and oxidative stress in human umbilical vein endothelial cells .…”

Assessment of in vivo antioxidant activity of a tripeptide Ala‐Tyr‐Ile fromJiuzao(a by‐product of baijiu distillation) protein hydrolysates and its stability in baijiu

“…Oxidative stress can cause a series of diseases (Chou et al., 2013; Shin et al., 2019; Zhang et al., 2019). Antioxidant ability as one of the main functions of bioactive peptides is widely studied (Hou et al., 2019; Tonolo et al., 2020).…”