“…The NCht was comprehensively reported to exhibit powerful antimicrobial actions for fighting microbial pathogens; the main action resulted from NCht surface charges (positive), which enable its attachment to microbial surfaces, deform them, and interfere their permeability [24,25,59]. The nanosized NCht particles could also penetrate into microbial cells and interact with their functional components (e.g., polymerization enzymes, DNA, RNAs, proteins, and enzymes) to inactivate them and prohibit well-organized cell membrane synthesis [24,60,61]. The He contains numerous bioactive constituents that have elevated antimicrobial and cytotoxicity properties against microbial cells, e.g., 1,4-naphthoquinone analogues, lawsone, esculetin, isoplumbagin, hennadiol, laxanthone, lupeol, lacoumarin, fraxetin, betulinic acid, scopoletin, betulin, and flavone glycosides [27,55,56,62]; most of these compounds have diverse microbicidal actions affected the synthesis and development of cells' vital and structural organelles.…”