Preparation of 1,3‐Diolein by Irreversible Acylation

Wang, Xiaosan; Han, Zhengyang; Zou, Wanzhen; Yang, Wan; Jin, Qingzhe

doi:10.1007/s11746-014-2579-y

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…The green synthesis of nanoparticles for enzyme immobilization has benefits to instigate the enhancement of the greater surface area, lower diffusion limitation, particle mobility, thermal stability, storage capacity, modulation of catalytic activity, cost-effective, low toxicity, effective preparation, and availability, and high productivity in terms of binding efficiency with enzymes. In the present work, to maximize the diolein yield and to improve the operational stability of the enzyme, a new synthesis was employed 20 , 21 . This work emphasizes on green route for the synthesis of Fe 2 O 3 (Iron (III) oxide) nanoparticles produced from Bauhinia tomentosa leaf extract and to synthesize 1,3 diolein using immobilized PPL.…”

Section: Introductionmentioning

confidence: 99%

One-pot green synthesis of iron oxide nanoparticles from Bauhinia tomentosa: Characterization and application towards synthesis of 1, 3 diolein

Lakshminarayanan

Shereen

Niraimathi

et al. 2021

Sci Rep

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The green synthesis of NPs through plant extracts can be a modest, one-pot alternative synthesis to the conventional physical or chemical method. The prime focus of this study is to produce MNPs by the reducing effect of Bauhinia tomentosa leaf extract, and it was immobilized in porcine pancreatic lipase (PPL). Synthesized NPs were characterized by field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) and Raman spectroscopy, UV–Vis Spectrometry, Thermogravimetry, and Differential Scanning Calorimeter (DSC), Zeta potential test, VSM, BET and Fourier Transform Infrared Spectroscopy (FTIR). The effect of process parameters was studied, about the efficiency of immobilization are enzyme stability, the extent of enzyme reusability, its separation from products, the activity of immobilized enzyme, recovery, and its loss. Finally, the immobilized lipase was used for the synthesis of 1,3-diolein using enzyme-mediated esterification of oleic acid and glycerol. Under optimized condition (reaction temp-55 $$^\circ $$ ∘ C; molar ratio-2.5:1; pH-7) diolein yield was achieved to be 94%. Therefore, this work was further used for the industrial production of 1,3-diacylglycerol since a perfect enzyme-catalyzed process was observed.

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Section: Introductionmentioning

confidence: 99%

One-pot green synthesis of iron oxide nanoparticles from Bauhinia tomentosa: Characterization and application towards synthesis of 1, 3 diolein

Lakshminarayanan

Shereen

Niraimathi

et al. 2021

Sci Rep

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“…Alternatively, glycerol can be replaced by 1,2‐acetonide glycerol to yield 1‐monoglyceryl esters of fatty acids (Wang et al., ) or the fatty acids (e.g. oleic acid) can be replaced by vinyl fatty acids (vinyloleate) (Wang et al., ).…”

Section: Assessmentmentioning

confidence: 99%

Re‐evaluation of mono‐ and di‐glycerides of fatty acids (E 471) as food additives

Younes¹,

Aggett²,

Aguilar³

et al. 2017

EFS2

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The EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS) provides a scientific opinion re-evaluating the safety of mono-and di-glycerides of fatty acids (E 471) when used as a food additive. The Panel considered that it is very likely that hydrolysis of mono-and di-glycerides of fatty acids by lipases in the gastrointestinal tract would occur, resulting in the release of glycerol and fatty acids. Glycerol (E 422) and fatty acids (E 570) have been re-evaluated and the Panel concluded that there was no safety concern regarding their use as food additives. Toxicological studies with mono-and di-glycerides rich in unsaturated fatty acids were considered for the re-evaluation of E 471. No evidence for adverse effects was reported in short-term, subchronic studies, chronic, reproductive and developmental toxicity studies. Neither carcinogenic potential nor a promotion effect in initiation/promotion was reported. The available studies did not raise any concern with regard to genotoxicity. The refined estimates were based on 31 out of 84 food categories in which E 471 is authorised. The Panel noted that the contribution of E 471 represented at the mean only 0.8-3.5% of the recommended daily fat intake. Based on the approach described in the conceptual framework for the risk assessment of certain food additives re-evaluated under Commission Regulation (EU) No 257/2010 and taking into account the considerations mentioned above, the Panel concluded that there was no need for a numerical acceptable daily intake (ADI) and that the food additive mono-and di-glycerides of fatty acids (E 471) was of no safety concern at the reported uses and use levels. The Panel recommended some modifications of the EU specifications for E 471.

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“…Moreover, they can prepare 1,3-diacylglycerol with unsaturated fatty acids such as oleic acid and linoleic acid that is commonly hard to produce by chemical methods. Accordingly, the enzymatic process for 1,3-diacylglycerol synthesis has been attracting increasing attention. − …”

Section: Introductionmentioning

confidence: 99%

Sustainable Process for 1,3-Diolein Synthesis Catalyzed by Immobilized Lipase from Penicillium expansum

Duan

Fang

Wang

et al. 2015

ACS Sustainable Chem. Eng.

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1,3-Diacylglycerol is conducive to reducing the increase of body fat and slowing the raise of body weight. So, growing attention has been paid to the enzyme-mediated 1,3diacylglycerol preparation due to the excellent selectivity and the environmental friendliness. In this work, we provided an alternative and sustainable process for 1,3-diacylglycerol synthesis catalyzed by immobilized lipase from Penicillium expansum (PEL) in which the esterification of oleic acid with glycerol for 1,3-diolein synthesis was employed as the model reaction. The results indicated that, under the optimum conditions (10.0 g of oleic acid, 1.09 g of glycerol, 80 U of immobilized PEL, and 6.4 g of 4 Å molecular sieves at 40 °C for 12 h), the diolein yield achieved to 82.4%, and the value of 1,3-diolien/diolein was 87.3%. Additionally, 92% original activity of the immobilized PEL was maintained after being used for 10 batches, highlighting the presumable cost-effectiveness of the enzyme. This study could be considered as an alternative and green strategy for preparing 1,3-diacylglycerol.

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Preparation of 1,3‐Diolein by Irreversible Acylation

Cited by 6 publications

References 29 publications

One-pot green synthesis of iron oxide nanoparticles from Bauhinia tomentosa: Characterization and application towards synthesis of 1, 3 diolein

One-pot green synthesis of iron oxide nanoparticles from Bauhinia tomentosa: Characterization and application towards synthesis of 1, 3 diolein

Re‐evaluation of mono‐ and di‐glycerides of fatty acids (E 471) as food additives

Sustainable Process for 1,3-Diolein Synthesis Catalyzed by Immobilized Lipase from Penicillium expansum

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