Sustainable Process for 1,3-Diolein Synthesis Catalyzed by Immobilized Lipase from Penicillium expansum

Abstract

1,3-Diacylglycerol is conducive to reducing the increase of body fat and slowing the raise of body weight. So, growing attention has been paid to the enzyme-mediated 1,3diacylglycerol preparation due to the excellent selectivity and the environmental friendliness. In this work, we provided an alternative and sustainable process for 1,3-diacylglycerol synthesis catalyzed by immobilized lipase from Penicillium expansum (PEL) in which the esterification of oleic acid with glycerol for 1,3-diolein synthesis was employed as the model reaction. The results indicated that, under the optimum conditions (10.0 g of oleic acid, 1.09 g of glycerol, 80 U of immobilized PEL, and 6.4 g of 4 Å molecular sieves at 40 °C for 12 h), the diolein yield achieved to 82.4%, and the value of 1,3-diolien/diolein was 87.3%. Additionally, 92% original activity of the immobilized PEL was maintained after being used for 10 batches, highlighting the presumable cost-effectiveness of the enzyme. This study could be considered as an alternative and green strategy for preparing 1,3-diacylglycerol.

Evaluation of the Candida sp. 99-125 Lipase Positional Selectivity for 1,3-Diolein Synthesis

“…1-Monoolein, 2-monoolein, 1,2-diolein, 1,3-diolein, and triolein contents in the reaction combination were examined according to the method of Duan et al [8]. Shimadzu 20A HPLC with ELSD (evaporative light scattering detector) was used.…”

Structure‐guided preparation of fuctional oil rich in 1,3‐diacylglycerols and linoleic acid from Camellia oil by combi‐lipase

“…In other words, the production of the DAG-enriched oil is affected by structureactivity relationship. In recent years, the synthesis of DAG with glycerol and FFA in terms of analytical purity has been investigated in numerous studies, 13,17,18,29 although most researchers focused on the effects of enzymatic systems and reactive mode systems for DAG productivity. However, high-purity oleic and LA (> 95%) are not easily obtained and additional purification energy is required during the actual production process.…”

Fabrication, characterization, and purification of nutraceutical diacylglycerol components from Camellia oil

“…Liu et al (2018) prepared an α-linolenic acid-rich DAG of 54.5% by enzymatic esterification. Interestingly, the esterification of oleic acid with glycerol achieved a DAG of over 80% theoretical yield by a 1,3-selective lipase (Duan et al, 2015;Wang et al, 2016).…”