Preparation of a novel injectable in situ-gelling nanoparticle with applications in controlled protein release and cancer cell entrapment

Abstract

At body temperature, thermosensitive nanoparticles release erythropoietin to lure metastatic cancer cells.

Design and applications of protein delivery systems in nanomedicine and tissue engineering

“…This is the common conditions used to obtain whey protein HGs, as the heating partially denatures the protein, leading to exposure of non-polar moieties and then to the aggregation of the protein in the form of HG [68]. More recently, the use of thermoresponsive particles that self-assemble into HG upon hydrophobic interaction through heating has also been reported [69]. Another possibility to induce physical cross-linking is a change of light intensity.…”

“…Advantages of this dye are an easy grafting and a low cost, however FITC is known to be highly sensitive to experimental parameters such as pH or photobleaching. Hence, other more stable labelling molecules have also been used on several proteins such as cyanine 5 on BSA, erythropoietin [69], RNBC [122], hepatocyte growth factor dimeric fragment (HGFdf) [144] and tumor necrosis factor-related apoptosis inducing ligand TRAIL [145], vivostag S750 on BMP2 [71],…”

Injectable Hydrogels for Cancer Therapy over the Last Decade

“…Hybrid materials were also prepared by incorporating carbon nanotubes [134] or Zn ferrite nanoparticles [135] for breast cancer treatment by combined DOX/photothermal and thermal ablation therapy, respectively. Injectable hydrogels, proposed for the thermo-responsive delivery of different drug molecules to prostate cancer in vivo, were prepared by radical polymerization of oligo(ethylene glycol) methacrylate (OEGMA) monomers [136]. In another study, PAA was combined with a poly[4-(2,2,6,6-tetramethyl piperidine- N -oxyl)aminomethylstyrene]– b –poly(ethylene glycol)– b –poly[4-(2,2,6,6-tetramethylpiperidine- N -oxyl)aminomethylstyrene] (PMNT–PEG–PMNT) triblock copolymer to obtain a redox-active polyion complex for the local protein therapy of murine colon cancer [137].…”

Carbon Nanotube–Liposome Complexes in Hydrogels for Controlled Drug Delivery via Near-Infrared Laser Stimulation

“…Hydrogels have been extensively studied and used for localized drug delivery due to their biocompatibility, modifiable properties, and high drug loading capacity. − The hydrogel drug delivery systems often utilize degradation rate or other physical/chemical parameters of the hydrogel scaffold as a tool to achieve a sustained and controlled release rate. However, many cancer conditions require more complex controlled release profiles (i.e., on-demand and real-time control over release). ,, Furthermore, conventional hydrogel systems can only be used to deliver hydrophilic drugs, leaving out a major group of drugs that are hydrophobic.…”