Preparation of a novel magnetic cellulose nanocrystal and its efficient use for enzyme immobilization

Abstract

Preparation of a novel magnetic cellulose nanocrystal via a simple co-precipitation-electrostatic-self-assembly technique and its efficient use for enzyme immobilization.

Papain@Magnetic Nanocrystalline Cellulose Nanobiocatalyst: A Highly Efficient Biocatalyst for Dipeptide Biosynthesis in Deep Eutectic Solvents

“…In this study, PA@MNCC was prepared using the following typical 37 . In addition, enzyme activity recovery was approximately 80.1%, and this value was a significant improvement compared with our previous study 10 .…”

“…Nanocrystalline cellulose was isolated from microcrystalline cellulose according to the method of Bondeson described in detail elsewhere [24][25] 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 6 incubated for 90 min at 80 o C. The resulting precipitate was washed with distilled water for 5 times and subjected to magnetic separation using a magnetic field. These collected MNCC materials were stored as stock suspensions with a solid content of 2% (w/v) prior to use.…”

“…NCC is generally prepared by acid-catalyzed hydrolysis of cellulose 5 , and has drawn increasing attention due to its excellent physicochemical properties (including high surface-to volume ratios, high aspect ratios, high stiffness, 3 good strength) and sustainability 6 . Glucose oxidase 7 , peroxidase 8 , and lysozyme 9 , as well as papain 10 were successfully immobilized onto NCC with enhanced activity, stability and catalytic efficiency. This indicated that the novel NCC-based enzyme carriers show great potential and are worthy of further study.…”

“…This indicated that the novel NCC-based enzyme carriers show great potential and are worthy of further study. It is noteworthy that the stable dispersion of NCC in the reaction system prevented the recycling and further application of enzymes immobilized onto the NCC-based carrier 10 . Thus, the incorporation of magnetic Fe 3 O 4 nanoparticles (MNPs) into the NCC polymer matrix may be a potential method of obtaining a well dispersed and easily separated NCC-based nanomaterial.…”

Immobilization of Tyrosinase in Nanocrystalline Cellulose/Chitosan Composite Film for Amperometric Detection of Phenol

“…The zeta potential was investigated at different pH value. It was observed that the zeta potential value at In a previous work, NCC and chitosan film was also prepared via electrostatic self-assembly due to electrostatic interaction between negatively charged NCC and positively charged chitosan [16]. The effect of a different amount of tyrosinase loading on the sensor response was also examined.…”