Preparation of a stannous methylene diphosphonate kit and its biological distribution in animals

Abstract

Summary A method is described for the preparation of a freeze‐dried stannous methylene diphosphonate kit* (Sn‐MDP) suitable for use in bone scanning after complexing with 99mTc. Several combinations of the reagents were used, and these resulted in different levels of bone and soft tissue localization. Two of such combinations produced a kit which localized predominantly in bone, and of these only one showed consistent batch to batch reproducibility. The biological distribution of the labelled compound has been tested in animals, and the results obtained at 2 h post‐injection are presented. The shelf‐life has been found to be in excess of 3 months, and quality control using thin layer chromatography has shown a labelling yield >95%.