Preparation of Amino Acid Conjugated Nano-Magnetic Particles for Delivery Systems

Abstract

Nanotechnologies to allow the nondisruptive introduction of carriers in vivo have wide potential for therapeutic delivery system. Iron oxide (γ-Fe 2 O 3 ) nano-magnetic particles (NMPs) were functionalized by silanization with (3-aminopropyl)triethoxysilane. For the purpose of functionalizing the surface of the particles with amino groups for subsequent cross-linking with pharmaceuticals and biomolecules. We have evaluated physical characterization of obtained NMPs by Fourier transform infrared spectroscopy, a superconducting quantum interference device, X-ray diffraction and transmission electron microscopy to confirm particle preparation. The particles were successfully introduced into living cells without any further modification to enhance endocytic internalization, such as the use of a cationic help. Furthermore, the functionalized NMPs were conjugated with an amino acid via a cross-linker, N -(ε-maleimidocaproyloxy) sulfosuccinimide ester, with the goal of selective uptake of NMPs by cells. To confirm the amino acid modification of the NMPs, the amino acid-conjugated NMPs were labeled with a fluorescent reagent (dansyl chloride), and the fluorescence was observed by confocal laser scanning microscopy. The amino acidconjugated NMPs have great potential for use in cell-selective delivery systems involving amino acid transporter proteins.

Nanotrap and Mass Analysis of Aromatic Molecules by Phenyl Group-Modified Nanoparticle

“…We have proposed a medical application that involves modifying the amino group, carboxyl group, and folic acid NPs for use in delivery systems described in previous reports. Modification of these functional groups were based on the silanization procedure . These functional NPs were introduced into live cells by endocytosis and through cell membrane receptors .…”

Low-Vacuum Deposition of Glutamic Acid and Pyroglutamic Acid: A Facile Methodology for Depositing Organic Materials beyond Amino Acids

“…Amino acids are expected to provide the biofriendly thin layers, on which biological microorganisms and biological molecular devices would be adequately immobilized maintaining their activities. Moreover, amino acids possessing multifunctional groups are promising motif molecules for constructing the well-ordered molecular networks for potential electro-optical applications [ 12 – 14 ]. Thermal stability of amino acids plays an important role in depositing thin solid films of amino acids by means of evaporation-sublimation processes.…”

Structural Characteristics and Ionization Ability of Manganese Oxide Nanoparticles

“…We have recently succeeded in producing novel nanoparticles as matrices for particles-assisted laser ionization/desorption mass spectrometry (PALDI-MS), and in carrying out clear analysis of molecules of small mass [1][2][3][4][5]. However, the mechanism of this ionization/desorption system is less well understood.…”