Preparation of an Acridinium Ester-Labeled Antibody and Its Application in GoldMag Nanoparticle-Based, Ultrasensitive Chemiluminescence Immunoassay for the Detection of Human Epididymis Protein 4

Ma, Ting; Zhang, Mengdan; Wan, Yinsheng; Cui, Yali; Ma, Le

doi:10.3390/mi8050149

Cited by 16 publications

(15 citation statements)

References 29 publications

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“…Moreover, they exhibit fast light emission with simple chemical triggers and their small size permit simple labeling protocols for proteins and nucleic acids . Some earlier studies have reported the applications of acridinium phenyl esters in immunoassays and nucleic acid assays and pointed toward their potential as useful labels in clinical diagnostics . In this study, we have established magnetic bead–based chemiluminescence immunoassay using acridinium phenyl esters as chemiluminescent labels for detecting the concentration of anti CPV2‐Ab in sample.…”

Section: Discussionmentioning

confidence: 99%

Determination of parvovirus antibodies in canine serum using magnetic bead‐based chemiluminescence immunoassay

Chen¹,

Guo²,

Hang³

et al. 2019

Biotech and App Biochem

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Canine parvovirus type 2 (CPV-2), as a highly contagious and potentially fatal disease of dogs and many other carnivores, usually causes severe gastroenteritis and myocarditis. Therefore, it is very necessary and urgent to have an accurate method to determine the CPV-2 antibodies (CPV-2-Ab) in canine samples. Here, a magnetic bead-based chemiluminescence immunoassay was established and optimized to detect the concentration of CPV-2-Ab in serum. And a commercial assay was also used to evaluate the consistency with our method. After optimization of the detective system, the CPV-2-Ab was captured by CPV-antigen-magnetic bead (8.3 µg/mL); then combined with the conjugation of anti-canine IgG antibody-acridinium ester (0.36 µg/mL). Finally, collected the signal (read the luminosity) after 1 H reaction time. The linear correlation coefficient (R 2 ) is 0.9924. The limit of detection (sensitivity) is 0.36 ng/mL (the linear dynamic range: 1.32-93.75 ng/mL), and the average recovery is 100.89% without cross-reactions with other canine viral antibodies. The results' correlation between commercial assays and this method is 0.9888. This immunoassay establishes that it has high sensitivity, accuracy, and specificity in clinical analysis, indicating that this method could be suitable for quantitative detection of CPV-2-Ab and evaluation of vaccination effect.

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Section: Discussionmentioning

confidence: 99%

Determination of parvovirus antibodies in canine serum using magnetic bead‐based chemiluminescence immunoassay

Chen¹,

Guo²,

Hang³

et al. 2019

Biotech and App Biochem

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“…Acridinium ester has been successfully used to perform ultrasensitive immunoassays of various proteins and antibodies. tumor markers (a-fetoprotein), thyroid stimulating hormone (TSH) and immunoglobulins (Ma et al, 2017;Min et al, 2018;Chen et al, 2019). Acridinium ester is also able to successfully label strands of DNA to produce DNA probes to emit CL (Komori et al, 2019).…”

Section: Acridinium Estersmentioning

confidence: 99%

Ultrasound-Enhanced Chemiluminescence for Bioimaging

Dhamecha

Gonsalves

et al. 2020

Front. Bioeng. Biotechnol.

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Tissue imaging has emerged as an important aspect of theragnosis. It is essential not only to evaluate the degree of the disease and thus provide appropriate treatments, but also to monitor the delivery of administered drugs and the subsequent recovery of target tissues. Several techniques including magnetic resonance imaging (MRI), computational tomography (CT), acoustic tomography (AT), biofluorescence (BF) and chemiluminescence (CL), have been developed to reconstruct three-dimensional images of tissues. While imaging has been achieved with adequate spatial resolution for shallow depths, challenges still remain for imaging deep tissues. Energy loss is usually observed when using a magnetic field or traditional ultrasound (US), which leads to a need for more powerful energy input. This may subsequently result in tissue damage. CT requires exposure to radiation and a high dose of contrast agent to be administered for imaging. The BF technique, meanwhile, is affected by strong scattering of light and autofluorescence of tissues. The CL is a more selective and sensitive method as stable luminophores are produced from physiochemical reactions, e.g. with reactive oxygen species. Development of near infrared-emitting luminophores also bring potential for application of CL in deep tissues and whole animal studies. However, traditional CL imaging requires an enhancer to increase the intensity of low-level light emissions, while reducing the scattering of emitted light through turbid tissue environment. There has been interest in the use of focused ultrasound (FUS), which can allow acoustic waves to propagate within tissues and modulate chemiluminescence signals. While light scattering is decreased, the spatial resolution is increased with the assistance of US. In this review, chemiluminescence detection in deep tissues with assistance of FUS will be highlighted to discuss its potential in deep tissue imaging.

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“…These advantages of CL-ELISA make it a useful detection system. Up to now, several chemiluminescent immunoassay methods have been established in the clinical detection of tumor markers, such as alpha-fetoprotein (AFP), prostate-speci c antigen (PSA), carbohydrate antigen 125 (CA125), and neuron-speci c enolase (NSH) (39)(40)(41)(42).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Sensitivity of VEGF Detection Using Catalase-Mediated Chemiluminescence Immunoassay Based on CdTe QD/H2O2 System

Ghavamipour

Rahmani

Shanehsaz

et al. 2020

Preprint

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Background: Since vascular endothelial growth factor (VEGF) is a major regulator of cancer angiogenesis, it is essential to develop a technology for its sensitive detection. Herein, we sensitized a chemiluminescence (CL) immunoassay through the combination of H 2 O 2 -sensitive TGA-CdTe quantum dot (QD) as signal transduction, dextran system as a cross-linker to prepare enzyme-labeled antigen and the ultrahigh bioactivity of catalase (CAT) as reporter enzyme. Results: Under the optimized experimental conditions, the CL-ELISA method can detect VEGF in the excellent linear range of 2–35000 pg mL -1 , with a detection limit (S/N = 3) of 0.5 pg mL -1 which was approximately 10 times lower than the commercial colorimetric immunoassay. This proposed method has been successfully applied to the clinical determination of VEGF in the human serum samples and the results illustrated the excellent correlation with the conventional ELISA method (R 2 = 0.997). The suitable recovery rate of the method in the serum ranged from 97% to 107%, with a relative standard deviation of 1.2% to 13.4%. Conclusions: The novel immunoassay proposes a highly sensitive, specific and stable method for very low levels detection of VEGF that can be used in primary diagnosis of tumor. With the well-designed sensing platform, this approach has a broad potential to be applied for quantitative analysis of numerous disease-related protein biomarkers for which antibodies are available.

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Preparation of an Acridinium Ester-Labeled Antibody and Its Application in GoldMag Nanoparticle-Based, Ultrasensitive Chemiluminescence Immunoassay for the Detection of Human Epididymis Protein 4

Cited by 16 publications

References 29 publications

Determination of parvovirus antibodies in canine serum using magnetic bead‐based chemiluminescence immunoassay

Determination of parvovirus antibodies in canine serum using magnetic bead‐based chemiluminescence immunoassay

Ultrasound-Enhanced Chemiluminescence for Bioimaging

Enhanced Sensitivity of VEGF Detection Using Catalase-Mediated Chemiluminescence Immunoassay Based on CdTe QD/H2O2 System

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