Preparation of anti-HER2 monoclonal antibody-paclitaxel immunoconjugate and its biological evaluation

Abstract

Anti-HER2 monoclonal antibody (Sc7301)-paclitaxel (TAX) immunoconjugate was prepared and its specific binding to tumor cells was investigated in this study. Sc7301 was conjugated to TAX by the active ester method and then the TAX-Sc7301 immunoconjugate was obtained. After purification and labeling by Cyano-fluorescein isothiocyanate (FITC), the specific binding of TAX-Sc7301 to HER2-positive tumor cells (SKOV3) and HER2-negative tumor cells (HepG2) was evaluated respectively. TAX-Sc7301 (20 nmol/L) showed distinct specific binding to SKOV3 cells rather than HepG2 cells. And the uptake of the immunoconjugate by SKOV3 cells was increased with the TAX-Sc7301 concentration (3-48 nmol/L) and the incubation time (P<0.05). It was concluded that the TAX-Sc7301 immunoconjugate is potentially applicable as a targeted agent against HER2-positive tumor cells.

RY10-4, a novel anti-tumor compound, exhibited its anti-angiogenesis activity by down-regulation of the HIF-1α and inhibition phosphorylation of AKT and mTOR

“…Phosphorylation of AKT and mTOR that were the important events in the activation of PI3 K/AKT/mTOR pathway had been reported in a variety of cancers. It can be modulated by the related genes such as HER2, PTEN [32,33]. mTOR, mammalian target of rapamycin, functions as a sensor of extra-cellular signals and then regulates angiogenesis, cell growth, apoptosis and autophagy, etc.…”

Development status and Prospect of directional hydraulic fracturing technology for coal mine roof

“…Li Quangui [16] and others designed different directional hole parameters and verified the auxiliary directional effect, which is beneficial to control the crack propagation. Chen Xuexi [17] et al made a comparative study on the hole arrangement modes of directional holes and non directional holes.…”

A Washing-Free and Easy-to-Operate Fluorescent Biosensor for Highly Efficient Detection of Breast Cancer-Derived Exosomes

“…In order to prove the high specificity of this biosensing strategy in the detection of SK-BR3 derived exosomes, we selected five different cancer cell-derived exosomes for specificity analysis. We learned from Figure 5 that, compared with high fluorescence signal produced by SK-BR3 derived exosomes, the low signals of three lower-relevant cancer cell-derived exosomes (cervical cancer cells: HeLa, prostate cancer cells: LNcap and liver cancer cells: HepG2) were almost as negligible as the blank (Dahl et al, 2011;Liu et al, 2011;Feng et al, 2012). As another breast cancer cell of which expression of HER2 on the cell membrane is far less than that of SK-BR3, the signal obtained by MCF-7 cell-derived exosomes was slightly increased but was also far lower than that of SK-BR3 (Baumann et al, 2016).…”