Preparation of bimetal–polydopamine organic frameworks with core–shell structure and their application in HER2 detection

Wu, Yawen; Chen, Xiaolong; Hou, Changjun; Lü, Peng; Lan, Ping; Li, Jiawei; Yang, Mei; Huo, Danqun

doi:10.1039/d1an01759d

Cited by 7 publications

(1 citation statement)

References 28 publications

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“…The interaction between ligands and targets was analyzed by nuclear magnetic resonance, molecular simulation, and X-ray diffraction. Complementary base pairs and other intermolecular interactions are the structural basis for determining ligands and targets. − Therefore, when a target appears, the adaptor forms a ″key lock″ complex with the target through spatial conformation, the superposition effect of various planar groups, electrostatic interaction, ion bond interaction, and hydrogen bond interaction to form a stable conjugate. − Trace disease biomarker detection in whole blood is the most significant and promising diagnostic method for diseases (e.g., aptamer targeted biomarkers). Current clinical testing of HER2 in blood involves polymerase chain reaction, a complex and time-consuming process that requires more than 4 h. This process can lead to the loss or contamination of ligands with a high false-positive rate.…”

Section: Introductionmentioning

confidence: 99%

Microswimmer-Based Ultrasensitive Dual-Signal Ratiometric Electrochemical Homogeneous Aptasensor with Functionalized Co-UiO-66 for the Single-Step Detection of HER2 in Whole Blood

Zhang

et al. 2023

Anal. Chem.

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The ability to efficiently detect trace disease biomarkers in whole blood remains an enormous challenge.Researchers have paid more attention to the homogeneous electrochemical ratio biosensor due to its self-calibration capability and improved detection accuracy. However, proportional homogeneous electrochemical sensing is difficult to achieve and typically requires functional modification of the electrode or the preparation of complex materials. Herein, a dual-signal ratiometric aptamer homogeneous electrochemical microswimming detection device with active capture capability and one-step detection of human epidermal growth factor receptor-2 (HER2) is proposed. The homogeneous electrochemical biosensor is fabricated based on a functionalized nanocomposite double-stranded DNA({singlestranded DNA-ferrocene (Fc)-aptamer})@Co-UiO-66 with catalase properties and adsorptive properties to electroactive toluidine blue (TB) molecules. Encapsulation of Co-UiO-66 material with dsDNA (ssDNA-Fc-Apt) containing HER2 aptamer as a gate switch inhibited its ability to adsorb TB molecules. This functionalized Co-UiO-66 material can catalyze hydrogen peroxide. Using hydrogen peroxide as a fuel, it breaks down to release oxygen bubbles, creating a propulsion force that drives dsDNA(ssDNA-Fc-Apt)@Co-UiO-66 target HER2 through whole blood. When the surface dsDNA (ssDNA-Fc-Apt)@Co-UiO-66 recognizes HER2, a strand displacement reaction occurs, and the ssDNA-Fc is released into solution. The HER2 aptamer is coiled because it targets HER2, and the ability to adsorb TB molecules is restored due to the exposed surface of Co-UiO-66. A certain negative voltage is applied to the ITO electrode, and due to the electrostatic attraction, the TB molecules and ssDNA-Fc are adsorbed and enriched on the surface of the electrode by electrostatic attraction, which produces two strong and oppositely changing electrochemical signals, and the electrochemical signals depend on the HER2 concentration. It can sensitively detect HER2 biomarkers in only 40 min with the detection range of 0.0001−10 ng/mL and detection limits as low as 10 fg/mL. The electrochemical microswimmer for the detection of trace disease biomarkers involves a one-step process of capture, signal change, and detection.

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Section: Introductionmentioning

confidence: 99%

Microswimmer-Based Ultrasensitive Dual-Signal Ratiometric Electrochemical Homogeneous Aptasensor with Functionalized Co-UiO-66 for the Single-Step Detection of HER2 in Whole Blood

Zhang

et al. 2023

Anal. Chem.

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A novel electrochemical biosensor based on AMNFs@ZIF-67 nano composite material for ultrasensitive detection of HER2

Zhang

et al. 2023

Bioelectrochemistry

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An ultra-sensitive dual-signal ratio electrochemical aptasensor based on functionalized bimetallic MOF nanocomplexes by the in-situ electrochemical synthesis for detect HER2

Zhang

et al. 2023

International Journal of Hydrogen Energy

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Preparation of bimetal–polydopamine organic frameworks with core–shell structure and their application in HER2 detection

Abstract: Human epidermal growth factor receptor 2 (HER2) is one of the specific markers of breast cancer, which is of great significance to the early diagnosis and prognosis of breast cancer....

Cited by 7 publications

References 28 publications

Microswimmer-Based Ultrasensitive Dual-Signal Ratiometric Electrochemical Homogeneous Aptasensor with Functionalized Co-UiO-66 for the Single-Step Detection of HER2 in Whole Blood

Microswimmer-Based Ultrasensitive Dual-Signal Ratiometric Electrochemical Homogeneous Aptasensor with Functionalized Co-UiO-66 for the Single-Step Detection of HER2 in Whole Blood

A novel electrochemical biosensor based on AMNFs@ZIF-67 nano composite material for ultrasensitive detection of HER2

An ultra-sensitive dual-signal ratio electrochemical aptasensor based on functionalized bimetallic MOF nanocomplexes by the in-situ electrochemical synthesis for detect HER2

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