2019
DOI: 10.1002/fsn3.1038
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Preparation of bioactive peptides with antidiabetic, antihypertensive, and antioxidant activities and identification of α‐glucosidase inhibitory peptides from soy protein

Abstract: In this study, the peptides of soy protein obtained by enzymatic digestion with proteases were analyzed for their antidiabetic, antihypertensive, and antioxidant activities. Peptides prepared with alkaline proteinase (AP) exhibited the highest α‐glucosidase inhibitory activity compared with those from papain and trypsin digestion. AP hydrolysates also exhibited dipeptidyl peptidase IV (DPP‐IV) inhibitory, angiotensin‐converting enzyme (ACE) inhibitory, and antioxidant activities. Gastrointestinal digestion of … Show more

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Cited by 157 publications
(103 citation statements)
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“…The highest DH value of hydrolysates was 17.5 ± 0.2%, which was obtained by alcalase, while that of the CGPH produced by flavourzyme was the lowest (2.05 ± 0.4%). Different DH value of each enzyme was due to different cleavage sites of the protease enzymes (Wang et al, 2019). Alcalase is an endo‐peptidase with a broad specificity, which favorably cleaves hydrophobic amino acid (tryptophan, phenylalanine, leucine, isoleucine, valine, and methionine) residues of peptide bonds, and higher DH value can be achieved with longer enzymolysis time.…”
Section: Resultsmentioning
confidence: 99%
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“…The highest DH value of hydrolysates was 17.5 ± 0.2%, which was obtained by alcalase, while that of the CGPH produced by flavourzyme was the lowest (2.05 ± 0.4%). Different DH value of each enzyme was due to different cleavage sites of the protease enzymes (Wang et al, 2019). Alcalase is an endo‐peptidase with a broad specificity, which favorably cleaves hydrophobic amino acid (tryptophan, phenylalanine, leucine, isoleucine, valine, and methionine) residues of peptide bonds, and higher DH value can be achieved with longer enzymolysis time.…”
Section: Resultsmentioning
confidence: 99%
“…Flavourzyme is an exo‐peptidase that breaks the N‐terminal of peptide chains. While trypsin cleaves exclusively C‐terminal to arginine and lysine (Ambigaipalan, Al‐Khalifa, & Shahidi, 2015; Wang et al, 2019), this result indicates that alcalase is the most efficient for corn germ protein hydrolysis. Similar results were obtained for whey protein (Lin, Tian, Li, Cao, & Jiang, 2012), rapeseed protein (He et al, 2013), camel milk casein (Kumar et al, 2016), hemp seed protein (Ren et al, 2016), quinoa and amaranth proteins (Lina, Omar, Kamal, Kilari, & Maqsood, 2019), and buffalo and bovine caseins (Shazly et al, 2019).…”
Section: Resultsmentioning
confidence: 99%
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“…Therefore, ACE inhibition is important to controlling the blood pressure. ACE-inhibitory peptides have been produced from various plant proteins such as cotton seed [Filho et al, 2020], soy protein [Wang et al, 2019], canola [Alashi et al, 2014], oat [Bleakley et al, 2017], peanut [Gong et al, 2017], and rice [Pinciroli et al, 2019].…”
Section: Introductionmentioning
confidence: 99%
“…According to the in silico structural model, these peptides bound to the substrate binding residues (Trp58, Trp59, Tyr62, Val163, His299, Asp300, and His305) and the catalytic residue (Asp300) of α-amylase with their active fragments (i.e., Asn-Leu-Pro-Arg-Met-Cys of P1 and P2, and Met-Phe-Ala-Gly-Ala of P3). Another example of α-glucosidase inhibitory peptides is those obtained from soy protein, with sequences LLPLPVLK, SWLRL, and WLRL and with IC50 of 237.43 ± 0.52, 182.05 ± 0.74, and 162.29 ± 0.74 μmol/L, respectively [ 63 ]. Furthermore, an α-glucosidase inhibitor was purified from Aspergillus oryzae N159-1.…”
Section: Peptides As Inhibitors Of Enzymes Involved In Metabolic Smentioning
confidence: 99%