Preparation of Biocompatible Liquid Marbles Stabilized by Food-Grade Stearate Microparticle for Aerobic Bacteria Cultivation

Tanaka, Shoma; Okano, Hiroaki; Matsuda, Nobuyuki; Sawai, Jun; Naoe, Kazumitsu; Imai, Masanao

doi:10.1007/s12010-020-03299-6

Cited by 13 publications

(18 citation statements)

References 29 publications

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“…Furthermore, the inner liquid can be released by disruption of the LMs via application of external stimuli, such as pH, temperature, magnetic field, and mechanical stress. [ 5 ] Thanks to these characters, researches on LMs have yielded various promising applications such as carriers of materials in microfluidics, [ 6,8–12 ] sensors, [ 13–17 ] miniature reactors for chemical and biological reactions, [ 3,7,18–24 ] and powdered pressure‐sensitive adhesives. [ 25 ]…”

Section: Introductionmentioning

confidence: 99%

Shape‐Designable Polyhedral Liquid Marbles/Plasticines Stabilized with Polymer Plates

Fujiwara

Geyer

Butt

et al. 2020

Adv Materials Inter

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Polyhedral liquid marbles/plasticines are prepared using (sub)millimeter‐sized polymer plates as a stabilizer and water as an inner liquid. Precise control of size and shape can be successfully performed by tuning the size ratio of the water droplet and the plate, number of plates adsorbed to the droplet, coalescence (jointing) of multiple polyhedral liquid marbles/plasticines, and application of external mechanical stress. Thanks to interfacial jamming of the plates, plastic deformation of the liquid marbles/plasticines is achieved. The authors are able to fabricate liquid marbles/plasticines with various shapes including A–Z letters of alphabet. Liquid marble/plasticine with an aspect ratio exceeding 800, the largest aspect ratio ever reported, is also successfully prepared; the length of the liquid marble/plasticine exceeded 1.5 m. The liquid marbles can be picked up and be piled up on top of each other using tweezers or fingers. Furthermore, Janus‐type liquid marbles/plasticines with different curvatures and different stabilizers in a single liquid marble/plasticine can be fabricated by coalescence (jointing) of near‐spherical and cuboid liquid marbles/plasticines stabilized by plates with different sizes. An internal liquid flow from the near‐spherical liquid marble to the cuboid liquid marble/plasticine immediately after jointing is observed, making this system act as a micropump.

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Section: Introductionmentioning

confidence: 99%

Shape‐Designable Polyhedral Liquid Marbles/Plasticines Stabilized with Polymer Plates

Fujiwara

Geyer

Butt

et al. 2020

Adv Materials Inter

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“…71 Especially, in the cultivation of aerobic microorganisms, culturing under a high A/V ratio is required. 8,72,73 The effect of the A/V ratio on BC production by the static cultivation of aerobic acetic acid bacteria has been investigated, and the optimum A/V ratio for BC production by the cells used has been reported. 39,41,74,75 Shaking culture and vigorous stirring were performed to increase the oxygen solubility in the culture solution; however, the cells were mutated by the shear stress caused by the stirring, resulting in low production yield.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…5 In particular, gas and vapor transport into or out of LMs makes them ideal chemical reactors for gas−liquid reactions 6 and cultivation media for aerobic microorganisms. 7,8 Recently, LMs have been deployed as CO 2 capture particles 9 and cell cultivation media. 10−13 Various solid particles have been used to prepare LMs.…”

Section: ■ Introductionmentioning

confidence: 99%

“…In fact, a higher proliferation of aerobic bacteria in the Ca stearate LM system has been achieved compared to conventional test tube systems. 8 Bacterial cellulose (BC) is a type of cellulose produced from bacteria and has attracted attention because of its extremely high purity as it does not involve biopolymers generally found in wood-derived cellulose, such as hemicellulose and lignin. 25 BC has a thinner fiber structure compared with that of woodderived cellulose and exhibits a distinct three-dimensional (3D) ultrafine fiber network.…”

Section: ■ Introductionmentioning

confidence: 99%

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Stearate Liquid Marbles for Bacterial Cellulose Production: Influence of the Liquid Marble Interface on Bacterial Cellulose Properties

et al. 2022

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We report aerobic bacteria culture performed in liquid marbles (LMs) for bacterial cellulose (BC) production. LMs are droplets stabilized by coating an aqueous solution with hydrophobic particles, which allow vapor and gas transfer through the liquid core−hydrophobic particle interface. We used safe foodgrade stearate microparticles for LM formation. An LM containing an aerobic acetic acid bacterium, Komagataeibacter xylinus (K. xylinus), which produces BC, was successfully prepared by dropping a culture medium solution inoculated with the bacteria onto a calcium stearate powder layer and rolling it to full coverage by calcium stearate microparticles. After the LMs containing the cells were statically cultured, the core solution became a hydrogel due to the production of BC by the cells. The obtained hydrogel (LM−BC gel) had a higher water holding capacity than that prepared by using a conventional test tube or Erlenmeyer flask. Scanning electron microscopy (SEM) observations showed that the BC fibers on the surface of the LM−BC gel were thicker than those prepared in conventional systems and formed a network structure with large holes, which was different from the conventional BC structure. We found that the BC prepared in LMs had the characteristic structure only on the outer surface of the gel. Considering that the bacterium movement is driven by the inverse force of the secretion of cellulose fibers, we found that the cells in the vicinity of the LM interface moved differently compared to that in conventional culture systems. The amount of BC produced per LM was linearly proportional to the core volume of the LM. In addition, changing the core volume significantly affected the surface area to volume ratio (A/V), whereas it did not affect the amount of BC produced per culture medium volume. Thus, the stearate LM system allows the production of BC by aerobic acetic acid bacteria, and the LM interface plays a critical role in the produced BC properties.

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“…11−13 For example, food-grade calcium stearate particles can be used to stabilize the liquid marbles for aerobic bacteria cultivation. 12 Hollow particles of sporopollenin were reported to prepare the liquid marbles at all pH values between 2 and 10. 13 Recently, stimulus-responsive liquid marbles have received increasing attention because of their potential applications in sensors, targeted release, and chemical reactor.…”

Section: ■ Introductionmentioning

confidence: 99%

pH-Responsive Liquid Marbles Based on Dihydroxystearic Acid

Zhang

Jiang

et al. 2022

Langmuir

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Herein, we report pH-responsive liquid marbles stabilized by 9,10-dihydroxystearic acid (DHSA). The particle morphology and the pH-responsive behavior of the liquid marbles were investigated. The rolling time during the preparation of liquid marbles has a great influence on the thickness of powder adsorption and the stability of the marbles. Compared with the liquid marbles stabilized by other fatty acids (e.g., stearic acid and docosoic acid), the liquid marbles prepared by DHSA have a much higher mechanical robustness. The increase in the number of hydroxyl groups on the carbon chain of fatty acids improves the mechanical robustness of the liquid marbles. Such liquid marbles immediately disintegrated on the surface of an alkaline solution or after exposure to NH 3 gas, which extends their applications in the NH 3 sensor and chemical reactions.

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Preparation of Biocompatible Liquid Marbles Stabilized by Food-Grade Stearate Microparticle for Aerobic Bacteria Cultivation

Cited by 13 publications

References 29 publications

Shape‐Designable Polyhedral Liquid Marbles/Plasticines Stabilized with Polymer Plates

Shape‐Designable Polyhedral Liquid Marbles/Plasticines Stabilized with Polymer Plates

Stearate Liquid Marbles for Bacterial Cellulose Production: Influence of the Liquid Marble Interface on Bacterial Cellulose Properties

pH-Responsive Liquid Marbles Based on Dihydroxystearic Acid

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