Preparation of Carbonated Apatite Membrane as Metronidazole Delivery System for Periodontal Application

Ardhani, Retno; Setyaningsih,; Hafiyyah, Osa Amila; Ana, Ika Dewi

doi:10.4028/www.scientific.net/kem.696.250

Cited by 11 publications

(5 citation statements)

References 6 publications

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“…Thus, the adjacent tissues will have a tensile strength in the normal range, because, with the weakness of the membrane, it will further limit the ability of the application, and become the burden for the body. 14,15 One of the shortcomings of this study was the measurement of gingival sulcus fluid volume did not carry out with the Perio-tron device. Periotron is a tool that can be used in research to check the levels of IL-1β.…”

Section: Discussionmentioning

confidence: 94%

The effect of carbonate apatite membrane gelatin application on IL-1β level gingival crevicular fluid in chronic periodontitis patients

2019

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Introduction:Apatite carbonate material plays an important role in bone tissue regeneration. The use of this membrane is expected to achieve better treatment success than those without additional therapy. The antimicrobial content in the apatite carbonate membrane can be used to support periodontal treatment of chronic periodontitis after scaling and root planing. The purpose of this research was to analyse the effect of carbonate apatite membrane gelatin application on the IL-1β level of the gingival crevicular fluid in chronic periodontitis patients. Methods: This research was a double-blind, randomised controlled trial method, with purposive sampling, and split-mouth design. The parameter was the IL-1β level in the gingival crevicular fluid before and after scaling and root planing. Carbonate apatite membrane gelatin was applied to the periodontal pocket on the test group after scaling and root planing. Data were analysed by the Wilcoxon test with a p-value < 0.05. Results: The reduction of IL-1β level gingival crevicular fluid on day-0 and day-30 on both groups showed significant value (p < 0.001). However, it showed no significant differences statistically, between the test group and the control group. Conclusion: Carbonate apatite membrane gelatin application might reduce the IL-1β level of the gingival crevicular fluid, but not showing a better reduction from the control group.

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Section: Discussionmentioning

confidence: 94%

The effect of carbonate apatite membrane gelatin application on IL-1β level gingival crevicular fluid in chronic periodontitis patients

2019

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“…The 10 μl of HBD3 with a concentration of 12.5 μg/l was loaded into 2 mg of membrane CHA. 8 This research is a study using 32 healthy and active Sprague Dawley male rats, weighing between 300 and 400 grams, and aged 3 to 4 months. Experimental animals, Sprague Dawley rats, were adapted in a clean cage for 7 days.…”

Section: Methodsmentioning

confidence: 99%

“…6 CHA bone grafts can also be combined with various antibiotics, for example, metronidazole. 8 The use of local and systemic antimicrobial therapy in extensive periodontal treatment is important as adjunctive therapy to prevent the growth and development of pathogenic bacteria. 9 Human β-defensin3 (HBD3) is an antimicrobial cationic peptide with an immunomodulatory effect on innate and adaptive immune responses.…”

Section: Introductionmentioning

confidence: 99%

The combination of carbonate hydroxyapatite and human β-defensin 3 to enhance collagen fibre density in periodontitis Sprague Dawley rats

et al. 2020

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Background: Carbonate hydroxyapatite (CHA) is used as a scaffold to repair bone resorption. Alveolar bone resorption in periodontitis caused by an infection requires the presence of an antibacterial to support bone regeneration. Human β-defensin 3 (HBD3) is an antimicrobial peptide. The local application of the HBD3 antimicrobial is beneficial to inhibiting drug resistance and protecting tissue regeneration against invasive bacteria. Purpose: This study aims to investigate the effect of the administration of a combination of CHA with HBD3 on the collagen density of periodontitis rats (Sprague Dawley). Methods: This study was a true experimental study with a post-test control group design. Thirty-two Sprague Dawley animal models were randomly blind selected placed under anaesthetic, then a 2-mm silk ligature was attached as a ligation to the mandibular incisors for 14 days in order to generate periodontitis. The study subjects were divided into two groups, the group with CHA and CHA loaded HBD3 (CHA + HBD3) implantation. On days 7, 14, 21 and 28, four rats were taken randomly from each group for decapitation, followed by histological processing and examination with trichome Mallory staining. The data was analysed using the Kruskal Wallis test (p<0.05). Results: An increase in collagen density during the healing process was found. There was a significant difference between CHA and CHA+HBD (p=0.004 and p=0.008; p<0.05) in collagen density between the groups. Conclusion: The combination of CHA and HBD3 can enhance the collagen density in periodontitis Sprague Dawley rats, compared to CHA only groups.

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“…The swelling ratio and degradation profile were evaluated using a UV-vis spectrophotometer (UV-1800, Shimadzu, Japan), referring to procedures published previously [21]. The PRP preparation and analysis of PRP-loading capacity and release profile were also conducted based on a previous study [21][22][23][24]. Before all in vitro cell and animal studies, the obtained SCMs were sterilized with ethylene oxide gas under dry conditions at 40 °C.…”

Section: Scm Preparationmentioning

confidence: 99%

“…Human PRP was obtained from the eligible voluntary donors who filled out and signed informed consent. Then, 10 mL of whole blood was collected from each donor and placed in a citrate Vacutainer (BD, NY, USA) for platelet counting [19,24]. The whole blood was then centrifuged for 10 min at 4 °C and 1700 rcf, and platelets were collected into another new tube.…”

Section: Preparation Of Prpmentioning

confidence: 99%

Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells with Synthetic Coral Matrix in the Presence or Absence of Growth Factors from the Platelet Rich-rich Plasma

Mahanani,

Ana,

Bachtiar

et al. 2023

Trends Sci

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To solve problems in using natural sea corals with high biocompatibility, good osteoconductivity, and ideal degradability, a synthetic three-dimensional coral matrix was fabricated and observed for its capability to enhance osteogenic differentiation of human mesenchymal stem cells (h-MSCs), in the presence or absence of growth factors (GFs) supplied from platelet-rich plasma (PRP). Expressions of runx, osterix, and osteocalcin were investigated, following cell attachment, and proliferation analysis. Ectopic bone formation in nonosseous tissues of Sprague-Dawley rats at predetermined time intervals was investigated, including mineralized tissue growth in vivo. The 3D synthetic coral matrix (SCM) can interact with the GF cocktail in the PRP and MSCs to generate and secret bone extracellular matrix (ECM) both in vitro and in vivo. The matrix supplied with GF cocktail from the PRP provided an ideal microenvironment for MSCs to attach, proliferate, and differentiate into osteoblast faster, as indicated by the expression levels of runx2, osterix, and osteocalcin. The high capability of SCMs to enhance bone formation has been proven by the formation of ectopic bones in the nonosseous environment. The incorporated PRP provided blood proteins such as fibrin to slow down matrix degradation, whereas GF supplied by the PRP stimulated h-MSCs to attach and proliferate onto the matrix. Moreover, the GF supplied by the PRP enhanced osteogenic differentiation and mineralization, accelerating bone regeneration. Valorization phases are needed to apply the SCM for bone tissue engineering in clinics. HIGHLIGHTS A synthetic coral matrix (SCM) with ideal macro- and microstructural appearance and physical and chemical properties, with tunable platelet-rich plasma (PRP)-loading capacity and release manner, was successfully fabricated The matrix provided an ideal microenvironment for human mesenchymal stem cells (h-MSCs) to attach, proliferate, and differentiate into osteoblasts indicated by high expression levels of bone regeneration markers The high capability of SCM to enhance bone regeneration has been validated by the formation of ectopic bones in the nonosseous environment of subcutaneous rat dorsal tissues The incorporation of a growth factor cocktail from the PRP did not alter the results in the matrix without growth factors, which act as signaling molecules The SCM was found to degrade faster in the group without h-MSCs, indicating that the system with cells promoted faster bone formation GRAPHICAL ABSTRACT

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Preparation of Carbonated Apatite Membrane as Metronidazole Delivery System for Periodontal Application

Cited by 11 publications

References 6 publications

The effect of carbonate apatite membrane gelatin application on IL-1β level gingival crevicular fluid in chronic periodontitis patients

The effect of carbonate apatite membrane gelatin application on IL-1β level gingival crevicular fluid in chronic periodontitis patients

The combination of carbonate hydroxyapatite and human β-defensin 3 to enhance collagen fibre density in periodontitis Sprague Dawley rats

Enhanced Osteogenic Differentiation of Human Mesenchymal Stem Cells with Synthetic Coral Matrix in the Presence or Absence of Growth Factors from the Platelet Rich-rich Plasma

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