Preparation of chemically stable allergen‐specific sublingual immunotherapy from Egyptian allergens

El‐Enin, Mohammed A. Abu; El‐Din, Eman M. Rabie Shehab; Abdelwahab, Heba Wagih; El‐Maksoud, Amina Abd; El‐Aziz, Abeer M. Abd; Shaaban, Mona I.; Attia, Ahmed; Aboukamar, Wafaa A.; Mohei‐Aldin, Sanaa; Belal, Fathalla

doi:10.1002/jcla.24261

Cited by 4 publications

(1 citation statement)

References 27 publications

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“…The SLIT manufactured according to Abu El-Enin et al [ 11 ], was given in suitable bottles with a dropper mechanism that permits comfortable dosing under the tongue. Dosing must be done in the morning.…”

Section: Methodsmentioning

confidence: 99%

Nondaily dosing schedule of allergen-specific sublingual immunotherapy: efficacy and safety

Abdelwahab

El-Maksoud

2023

Clin Exp Vaccine Res

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Purpose Sublingual immunotherapy is currently promoted by various companies, with administration schedules variable in the different products even though almost all are standardized immunologically. So, this study was planned to examine the efficacy of simple nondaily dosing of sublingual immunotherapy instead of the widely used daily schedule. Materials and Methods Fifty-two patients with allergic rhinitis and bronchial asthma were enrolled. Sublingual immunotherapy (manufactured at the allergen immunotherapy preparation unit at Mansoura University) was given in suitable bottles with a dropper mechanism that permits comfortable dosing under the tongue. The physician recommended that the patient put the drops under his/her tongue and leave the drops beneath the tongue for 2 minutes before swallowing. This was repeated every 3 days, with the drop number and concentration gradually rising. Results After 2 months of follow-up, 65.8% responded partially to the symptom score and 26.3% responded completely to the medication score. There was a significant decline in the symptom and medication scores from the baseline scores (p<0.0001). After 4 months of follow-up, 95.8% responded partially to symptom scores and no one has not responded; 54.2% responded completely to medication scores; and 81% of studied patients had no side effects. However, the most frequent side effect was a sore throat. Conclusion Our nondaily schedule of sublingual immunotherapy is tolerable, safe, and effective in patients with allergic rhinitis and bronchial asthma.

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Section: Methodsmentioning

confidence: 99%

Nondaily dosing schedule of allergen-specific sublingual immunotherapy: efficacy and safety

Abdelwahab

El-Maksoud

2023

Clin Exp Vaccine Res

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Preparation of chemically stable allergen‐specific sublingual immunotherapy from Egyptian allergens

El‐Enin

El‐Din

Abdelwahab

et al. 2022

Clinical Laboratory Analysis

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Background:The term "allergen extracts" refers to solutions of proteins or glycoproteins extracted from source raw materials.Objectives: This study was planned to prepare chemically stable sublingual immunotherapy from different allergens in Egypt. Methods:Allergen extraction from raw materials. The concentrated aqueous extract of each allergen was mixed with an equal volume of glycerol. The protein content of the preparations was determined using the modified Lowry assay method. The prepared allergens were stored for 9 months at 2-4°C. Samples were analyzed periodically (0, 3, 6, and 9 months of intervals) adopting the Lowry Assay method. Levels of specific IgE to Chenopodium album antigens were measured in patients' sera by ELISA. Results:The concentration of all prepared allergens, as indicated by the concentration of the protein content, was found to decrease exponentially with time, implying first-order kinetics of degradation. From the values of the slopes of the log plot for each allergen, the half-life time (t 1/2 ) and (t 1/4 ) values were calculated. The expiration date was considered as the time after which the allergen loses 25% of its potency. The obtained values of t 1/4% vary according to the type of vaccine. The most stable one is that of Chenopodium album pollens (2.4 years) and the least stable is that of house dust Mites (9 months). The immunological characters of Chenopodium album extract were stable for at least 6 months. Conclusion:Differences exist among allergen extracts made by multiple manufacturers. So, developments in studies on allergen preparation and characterization in a different locality are necessary.

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Allergen sensitization patterns concerning different governorates in Egypt: a retrospective study

Abdelwahab,

El-Maksoud,

Elhosiny

et al. 2024

Egypt J Chest Dis Tuberc

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Background Allergic disorders afflict 22% of the world’s population. Treatment and prevention of allergic diseases are dependent on correctly identifying the causal allergens and implementing appropriate avoidance approaches. We aimed to assess the variation in patterns of allergen sensitization among patients with allergic disorders in different Egyptian governorates. Methods This retrospective study included patients with allergic disorders attending an allergen immunotherapy outpatient clinic. Demographic data, diagnosis of allergic disorders, and skin prick test results of enrolled patients were retrieved from the medical records. Also, patients’ place of residence was recorded by the governorate. Results This study included 5024 patients with allergic disorders. Most of the studied patients were sensitized to mold (94.3%) followed by house dust mites (74.7%) and hay dust (69%). There was no statistical difference in allergen sensitization patterns in different Egyptian governorates. However, there was a statistically significant relation between allergic rhinitis and mixed pollen 1 (P value 0.02) and mite (P value 0.001). For bronchial asthma, both dog hair and chicken feather sensitization patterns showed a strong association with P values 0.02 and 0.03, respectively. Atopic dermatitis had a significant association with both mites (P value 0.001) and barely (P value 0.02). Conclusion The sensitization patterns did not significantly differ between the Egyptian governorates. However, it is significantly associated with allergic disorders. The most prevalent sensitization pattern in Egypt was mold.

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Preparation of chemically stable allergen‐specific sublingual immunotherapy from Egyptian allergens

Cited by 4 publications

References 27 publications

Nondaily dosing schedule of allergen-specific sublingual immunotherapy: efficacy and safety

Nondaily dosing schedule of allergen-specific sublingual immunotherapy: efficacy and safety

Preparation of chemically stable allergen‐specific sublingual immunotherapy from Egyptian allergens

Allergen sensitization patterns concerning different governorates in Egypt: a retrospective study

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