Preparation of Chitosan-Gelatin Based Sponge Cross-Linked with GlcNAc for Bone Tissue Engineering

Chaochai, Thitirat; Miyaji, Hirofumi; Yoshida, Takashi; Nishida, Erika; Furuike, Tetsuya; Tamura, Hiroshi

doi:10.1166/jcc.2016.1096

Cited by 11 publications

(6 citation statements)

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“…e peak at 1650 cm −1 corresponds to the stretching vibration of C�O (amide I). e peak at 1540 cm −1 is induced by the bending and stretching vibration of N-H (amide II), and the peak at 1240 cm −1 is attributed to the bending of N-H. ese absorption peaks are characteristic of gelatin [39,55]; therefore, the presence of these peaks proves that the electrospinning process did not alter the functional groups of the gelatin.…”

Section: Resultsmentioning

confidence: 98%

Preparation and Characterization of Electrospun Gelatin Nanofibers for Use as Nonaqueous Electrolyte in Electric Double-Layer Capacitor

Kotatha

Hirata

Ogino

et al. 2019

Journal of Nanotechnology

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A novel nanofibrous gel electrolyte was prepared via gelatin electrospinning for use as a nonaqueous electrolyte in electric double-layer capacitors (EDLCs). An electrospinning technique with a 25 wt% gelatin solution was applied to produce gelatin electrospun (GES) nanofiber electrolytes. Structural analysis of the GES products showed a clearly nanofibrous structure with fiber diameters in the 306.2–428.4 nm range and exhibiting high thermal stability, high tensile strength, and a stable form of nanofibrous structure after immersion in 1-ethyl-3-methylimidazolium tetrafluoroborate (EMImBF4). After testing over a range of spinning times, GES electrolytes that were produced at 25 min (GES-25) had a suitable thickness for the assembly of EDLC with the optimized tensile properties and were used to fabricate EDLC test cells with EMImBF4. These test cells were compared to those with pure EMImBF4 and a separator as an electrolyte. The electrochemical properties of the test cells were characterized by charge-discharge testing, discharge capacitance, and alternative current (AC) impedance measurements. AC impedance measurements showed that the test cell with the GES-25/EMImBF4 gel electrolyte showed slightly poorer contact with the electrode when compared to that with pure EMImBF4, whereas exhibited comparable IR drop and discharge capacitance (calculated capacitance retention was 56.6%). The results demonstrated that this novel gel electrolyte can be used as a nonaqueous electrolyte in order to improve the safety in EDLCs.

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Section: Resultsmentioning

confidence: 98%

Preparation and Characterization of Electrospun Gelatin Nanofibers for Use as Nonaqueous Electrolyte in Electric Double-Layer Capacitor

Kotatha

Hirata

Ogino

et al. 2019

Journal of Nanotechnology

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“…But, the surface of the GA‐modified gelatin showed a porous network structure which was formed due to the sublimation of ice in the frozen gelatin samples generated during the freeze‐drying processing (Kang, Tabata, & Ikada, ). Therefore, GA‐modified gelatin was used to prepare porous gelatin scaffolds in the field of tissue engineering (Agarwal et al, ; Chaochai et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…the freeze-drying processing (Kang, Tabata, & Ikada, 1999). Therefore, GA-modified gelatin was used to prepare porous gelatin scaffolds in the field of tissue engineering (Agarwal et al, 2016;Chaochai et al, 2016).…”

Section: Sem Analysismentioning

confidence: 99%

Controlled release of methylene blue from glutaraldehyde‐modified gelatin

Zhu

Zhang

et al. 2019

J Food Biochem

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Gelatin has been identified as a valuable material for controlled release of food ingredients because of its highly ramified three‐dimensional network. In the present work, gelatin was further modified with glutaraldehyde (GA) and prepared as microspheres to further improve its control to release of food ingredients. Features (i.e., chemical structures, thermal properties, network structures, and methylene blue (MB) release kinetics) of both the unmodified and GA‐modified gelatin microspheres were investigated. Results showed that the application of GA enhanced the formation of intramolecular and/or intermolecular crosslinking of gelatin and improved its thermal stability. Besides, the release efficiency of MB from the GA‐modified gelatin was less affected by temperature and pH value compared to the unmodified gelatin. Kinetics analysis proved that the release of MB from gelatin followed the first‐order kinetics. Both the release constant (k) of MB and the diffusion coefficient (Da) of GA‐modified gelatin were significantly lower than those of the unmodified gelatin. Practical applications Gelatin is a valuable material for the release control of food ingredients (e.g., oils, vitamins, antioxidants, and flavor compounds) attributes to its highly ramified three‐dimensional network. However, the low mechanical strength of gelatin makes it susceptible to many environmental factors and has restricted its application. In the present work, gelatin was modified by glutaraldehyde (GA). The chemical structure, thermal properties, network structure, and methylene blue release kinetics of the GA‐modified gelatin microspheres were properly investigated. This research proved that GA‐modified gelatin could be a potential carrier for the release control of given food contents in food industry.

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“…Gelatin (Gel) is a mixture of peptides and proteins produced by partial acid or alkaline hydrolysis of collagen. Gel is biocompatible and shows low antigenicity [1], in contrast to collagen, which has greater antigenicity because of its animal origin [2] [3]. In addition, chitosan is well investigated by the biomaterial, food, and chemical industries because of its good biocompatibility, biodegradability, hemostasis, and other properties [4]- [9].…”

Section: Introductionmentioning

confidence: 99%

“…In this study, we examined Gel/CTS sponges developed by crosslinking with GlcNAc. According to the Maillard reaction, crosslinking GlcNAc and amino group produces browning compounds due to the interaction between carbonyl group, reducing sugar, and the amino compound [1]. Protein adsorption is very important in biomedical research.…”

Section: Introductionmentioning

confidence: 99%

Adsorption and Desorption Behaviors of Bovine Serum Albumin on Gelatin/Chitosan Sponge

Furuike¹,

Chaochai²,

Komoto³

et al. 2017

MSCE

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Gelatin (Gel) and chitosan (CTS) have several biomedical applications because of their biodegradability and biocompatibility. Crosslinking of Gel and Gel/CTS systems was evaluated using N-acetyl-D-glucosamine (GlcNAc) formed into sponges by lyophilization. The prepared sponges were used to study the adsorption and desorption of fluorescein isothiocyanate (FITC) labeled bovine serum albumin (BSA) as a model instead of a growth factor. The effect of FITC-BSA concentration and temperature on the adsorption behavior of Gel/CTS sponges was investigated. The Langmuir adsorption isotherm model was used on the basis of the assumption that monolayer adsorption occurs on the surface; the results fit with the experiment data. The adsorption constants were 5.77 and 9.68 mL/mg for Gel and Gel/CTS sponges, respectively. The adsorption thermodynamic constants were found; adsorption onto sponges was an exothermic reaction. In particular, Gibbs free energy (ΔG) exhibited negative values in the range of 283-343 K for both Gel and Gel/CTS sponges, demonstrating the spontaneous nature of adsorption reaction. In addition, desorption behavior was evaluated for different concentrations and pH values of the FITC-BSA solution. The high adsorbed amounts of FITC-BSA on sponge resulted in high desorbed amounts in sponge, up to 55% from 3.5 mg/mL adsorbed concentration (around 1.5 mg from 3 mg adsorb amount). Desorption decreased following the buffer solution pH decrease, from 7.4 to 4 and 2 in Gel and Gel/CTS sponges, respectively. Based on the results of this preliminary study, these composite sponges could have significant application in biomedical materials.

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Preparation of Chitosan-Gelatin Based Sponge Cross-Linked with GlcNAc for Bone Tissue Engineering

Cited by 11 publications

References 0 publications

Preparation and Characterization of Electrospun Gelatin Nanofibers for Use as Nonaqueous Electrolyte in Electric Double-Layer Capacitor

Preparation and Characterization of Electrospun Gelatin Nanofibers for Use as Nonaqueous Electrolyte in Electric Double-Layer Capacitor

Controlled release of methylene blue from glutaraldehyde‐modified gelatin

Adsorption and Desorption Behaviors of Bovine Serum Albumin on Gelatin/Chitosan Sponge

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