Preparation of Chitosan Nanoparticles and their In-vitro Characterization

Agarwal, Megha; Agarwal, Megha; Shrivastav, Nalini; Pandey, Sarika; Das, Ritu; Gaur, Priyanka

doi:10.21276/ijlssr.2018.4.2.17

Cited by 104 publications

(72 citation statements)

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“…Regarding CS and CS derivatives' spectra, all the characteristic peaks of them were observed. Nevertheless, consistent with previous works, particles formulated with TPP polyanion via an ionic gelation technique show shifts in their FTIR spectra in the region of amides (amide I and II) and phosphate groups of TPP, with the latter recorded at 897 cm −1 [67]. Not only for CS/TPP laden particles, but also for CS derivatives' formulations, the abovementioned peak was accordingly shifted to lower wavenumbers indicating the interactions between amino and phosphate groups.…”

Section: Characterization Of Mpssupporting

confidence: 90%

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Microencapsulation of Fluticasone Propionate and Salmeterol Xinafoate in Modified Chitosan Microparticles for Release Optimization

et al. 2020

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Chitosan (CS) is a natural polysaccharide, widely studied in the past due to its unique properties such as biocompatibility, biodegradability and non-toxicity. Chemical modification of CS is an effective pathway to prepare new matrices with additional functional groups and improved properties, such as increment of hydrophilicity and swelling rate, for drug delivery purposes. In the present study, four derivatives of CS with trans-aconitic acid (t-Acon), succinic anhydride (Succ), 2-hydroxyethyl acrylate (2-HEA) and acrylic acid (AA) were prepared, and their successful grafting was confirmed by FTIR and 1H-NMR spectroscopies. Neat chitosan and its grafted derivatives were fabricated for the encapsulation of fluticasone propionate (FLU) and salmeterol xinafoate (SX) drugs, used for chronic obstructive pulmonary disease (COPD), via the ionotropic gelation technique. Scanning electron microscopy (SEM) micrographs demonstrated that round-shaped microparticles (MPs) were effectively prepared with average sizes ranging between 0.4 and 2.2 μm, as were measured by dynamic light scattering (DLS), while zeta potential verified in all cases their positive charged surface. FTIR spectroscopy showed that some interactions take place between the drugs and the polymeric matrices, while X-ray diffraction (XRD) patterns exhibited that both drugs were encapsulated in MPs’ interior with a lower degree of crystallinity than the neat drugs. In vitro release studies of FLU and SX exposed a great amelioration in the drugs’ dissolution profile from all modified CS’s MPs, in comparison to those of neat drugs. The latter fact is attributed to the reduction in crystallinity of the active substances in the MPs’ interior.

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Section: Characterization Of Mpssupporting

confidence: 90%

“…where w1, w2 and w3 are the initial weight of neat CS, the grafted weight of CS derivative and the weight of the added monomer respectively [67].…”

Section: Grafting Percentage and Grafting Efficacymentioning

confidence: 99%

Microencapsulation of Fluticasone Propionate and Salmeterol Xinafoate in Modified Chitosan Microparticles for Release Optimization

et al. 2020

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“…high levels of stability. Therefore, in our study according to the past studies negative f-potential (À44.5 ± 0.57 mV, À54.4 ± 0.71 mV) increases the electrostatic repulsion and increases the dispersion of magnetic nanoparticles in water and reduces aggregation of magnetic nanoparticles [44,45].…”

Section: Discussionsupporting

confidence: 54%

HBs antigen and mannose loading on the surface of iron oxide nanoparticles in order to immuno-targeting: fabrication, characterization, cellular and humoral immunoassay

Rezaei

Hosseini

Khavari-Nejad

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Mannosylation of nanovaccine is an appropriate strategy for targeting the mannose receptors on DCs. Here, HBsAg and mannose loaded on the surface of iron oxide nanoparticles to increases HBsAg vaccine potency. Nanoparticles are made by co-precipitation method and bonded to the HBsAg and mannose by chemical bonding. The physicochemical properties of nano-vaccines, their toxicity and antigenicity were determined. The synthesized nano-vaccine showed spherical shape with a mean particle size of 60 nm, a zeta potential of À44 mV, an antigen-binding efficiency of around 100% and for mannose 78%. In vitro release of nanoparticles exhibited about 30% at the first day and about 60% until the third day. SDSPAGE analysis confirmed structural integrity of HBsAg loaded on nanoparticles. The HBsAg-loaded LCMNP and MLCMNP nanoparticles had no toxic effects on HEK293 cell line. The quantification of the intracellular Fe by ICP-OES as a criterion of nano-vaccine uptake revealed mannose intensify uptake of MLCMNP. In addition, mannose in the structure of MLCMNP improved IL-6, TNF-a and IFN-c (>16 fold) cytokines genes expression by macrophage/dendritic cells after exposure in 12 h. Immunization of experimental mice (subcutaneously, two times with 2-week intervals) with 5 mg of HBsAg loaded on MLCMNP nanoparticles increased specific total IgG and IgG2a/IgG1 ratio. In addition, TNF-a, IL-12, IL-2 and IL-4 cytokines in mannosylated nano-vaccine increased versus nano-vaccine group while lymphocyte proliferation and IFN-c responses in the targeted nano-vaccine group show a tiny increase versus the nano-vaccine group. The results show that mannosylated nano-vaccine promotes higher level of cellular and humoural immune responses against HBsAg nano-vaccine.

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“…The above prepared solution was constantly kept in magnetic stirrer to this, a mixture of TPP was added dropwise with constant stirring and allowed for 30 minutes. (Grenha, 2012;Fàbregas et al, 2013;Kunjachan and Jose, 2010;Agarwal et al, 2018;Papadimitriou et al, 2008) Chitosan and TPP were added in the ratio of 6:1. Table 1 shows nine different formulations with three different ratios of chitosan and TPP for moda inil nanoparticles.…”

Section: Formulation Of Moda Inil Nanoparticlesmentioning

confidence: 99%

Enhancement of solubility and bioavailability of modafinil using nanoparticles

Balachandar

Subramanian

2020

ijrps

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The aim of the present work is to develop polymeric nanoparticles using Modafinil to improve its solubility, thereby increasing its bioavailability. Ionic-Gelation method has been employed for the preparation of nanoparticles using chitosan, a natural polymer, and TPP, a cross-linking agent. In this method, the drug is mixed with a polymer and maintained at a pH between 4.5 - 5.5, and the solution of TPP is added to the polymer-drug mixture under magnetic stirring. The size of nanoparticles prepared by this method usually ranges from 1-1000 nm. The reduction in the size of particles directly enhances the solubility of drug that increases its bioavailability. Nine formulations (F1-F9) of nanoparticles were optimized by varying the concentrations of chitosan and TPP. Prepared nanoparticles were evaluated for size, zeta potential, PDI, and drug content. Formulation F9 with 0.25% of chitosan & 0.5% of TPP concentration has been chosen as best formulation and lyophilized to obtain solid nanoparticles. The obtained nanoparticles loaded with modafinil are used for the preparation of tablets, and the in-vitro dissolution characteristics are compared with the marketed tablet of modafinil. in-vitrocharacteristics of tablets with modafinil loaded polymeric nanoparticles showed improved solubility than a marketed tablet.

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Preparation of Chitosan Nanoparticles and their In-vitro Characterization

Cited by 104 publications

References 0 publications

Microencapsulation of Fluticasone Propionate and Salmeterol Xinafoate in Modified Chitosan Microparticles for Release Optimization

Microencapsulation of Fluticasone Propionate and Salmeterol Xinafoate in Modified Chitosan Microparticles for Release Optimization

HBs antigen and mannose loading on the surface of iron oxide nanoparticles in order to immuno-targeting: fabrication, characterization, cellular and humoral immunoassay

Enhancement of solubility and bioavailability of modafinil using nanoparticles

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