Preparation of chitosan nanoparticles containing Naja naja oxiana snake venom

Mohammadpourdounighi, Naser; Behfar, Azam; Ezabadi, Ali; Zolfagharian, Hossein; Heydari, Maryam

doi:10.1016/j.nano.2009.06.002

Cited by 53 publications

(38 citation statements)

References 37 publications

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“…The results of FTIR analysis of CS/TPP nanoparticles showed a high peak of 1738 cm −1 in CS, indicating the presence of NH 2 group. The signal intensity for the NH 2 decreased to 1643 cm −1 after addition of TPP, as observed by Lam et al and Mohammadpourdounighi et al . Lam et al observed the peaks of 1650 cm −1 and 1636 cm −1 for amino group in CS and CS/TPP, respectively .…”

Section: Discussionsupporting

confidence: 65%

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Synthesis and Characterization of Chitosan Tripolyphosphate Nanoparticles and its Encapsulation Efficiency Containing Russell's Viper Snake Venom

Venkatesan¹,

Vimal²,

Hameed³

2013

J Biochem & Molecular Tox

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Chitosan Tripolyphosphate (CS/TPP) nanoparticle is a biodegradable and nontoxic polysaccharide, used as a carrier for drug delivery. The morphology and particle-size measurements of the nanoparticles were studied by field emission scanning electron microscopy and Fourier Transform Infrared Spectroscopy (FTIR). This study aims to evaluate the impact of Russell's viper venom encapsulation on various factors and loading capacity, in addition to explore the physicochemical structure of nanoparticles. FTIR confirmed that tripolyphosphoric groups of TPP linked with ammonium groups of CS in the nanoparticles. Our results showed that CS can react with TPP to form stable cationic nanoparticles. The results also showed that encapsulation efficiency of venom at different concentrations of 20, 40, 60, 500, and 1000 µg/mL were achieved for CS/TPP nanoparticles at different concentrations of 1.5, 2, and 3 mg/mL. The cytotoxicity of CS/TPP nanoparticles was evaluated by MTT (-3 (4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a tetrazole) assay.

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Section: Discussionsupporting

confidence: 65%

“…CS/TPP nanoparticles were prepared by ionic gelation process following the protocol described by Mohammadpourdounighi et al . CS was dissolved at a concentration of 3 mg/mL in 0.5% acetic acid solution.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Characterization of Chitosan Tripolyphosphate Nanoparticles and its Encapsulation Efficiency Containing Russell's Viper Snake Venom

Venkatesan¹,

Vimal²,

Hameed³

2013

J Biochem & Molecular Tox

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“…NPs could form spontaneously in mixed TPP and chitosan solutions through inter and intra molecular linkages created between TPP phosphates and chitosan amino groups (Calvo et al, 1997). These types of nanoparticulate systems have shown a high affinity for the association of negatively charged macromolecules (Mohammadpourdounighi et al, 2010), such as the mucin that are present on the mucosal surface.…”

Section: Introductionmentioning

confidence: 99%

Engineering tenofovir loaded chitosan nanoparticles to maximize microbicide mucoadhesion

Meng

Sturgis

Youan

2011

European Journal of Pharmaceutical Sciences

154

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The objective of this study was to engineer a model anti-HIV microbicide (Tenofovir) loaded chitosan based nanoparticles (NPs). Box-Behnken design allowed to assess the influence of formulation variables on the size of NPs and drug encapsulation efficiency (EE%) that were analyzed by dynamic light scattering and UV spectroscopy, respectively. The effect of the NPs on vaginal epithelial cells and Lactobacillus crispatus viability and their mucoadhesion to porcine vaginal tissue were assessed by cytotoxicity assays and fluorimetry, respectively. In the optimal aqueous conditions, the EE% and NPs size was 5.83% and 207.97nm, respectively. With 50% (v/v) ethanol/water as alternative solvent, these two responses increased to 20% and 602 nm, respectively. Drug release from medium (281 nm) and large size (602 nm)-sized NPs fitted the Higuchi (r2=0.991) and first-order release (r2=0.999) models, respectively. These NPs were not cytotoxic to both the vaginal epithelial cell line and Lactobacillus for 48 hours. When the diameter of the NPs decreased from 900 nm to 188 nm, the mucoadhesion increased from 6% to 12%. However, the combinatorial effect of EE% × mucoadhesion for larger size NPs was the highest. Overall, large-size, microbicide loaded chitosan NPs appeared to be promising nanomedicines for the prevention of HIV transmission.

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“…The product was characterized according its viscosity average molar mass = 977 kDa by capillary viscometry and degree of acetylation = 10.5% by nuclear magnetic proton resonance using conditions described elsewhere . Chitosan‐tripolyphosphate (TPP) particles loaded with triclosan (2,4,4‐trichloro‐2‐hydroxy diphenyl ether) (Calbiochem, San Diego, CA, USA) were prepared according to a previous study . Briefly, chitosan (3.0 mg/mL) and triclosan (0.06 mg/L) were dissolved in an acetic aqueous solution (3 mg/mL).…”

Section: Methodsmentioning

confidence: 99%

Chitosan‐triclosan particles modulate inflammatory signaling in gingival fibroblasts

Pavéz

Tobar

Chacón

et al. 2017

J of Periodontal Research

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Preparation of chitosan nanoparticles containing Naja naja oxiana snake venom

Cited by 53 publications

References 37 publications

Synthesis and Characterization of Chitosan Tripolyphosphate Nanoparticles and its Encapsulation Efficiency Containing Russell's Viper Snake Venom

Synthesis and Characterization of Chitosan Tripolyphosphate Nanoparticles and its Encapsulation Efficiency Containing Russell's Viper Snake Venom

Engineering tenofovir loaded chitosan nanoparticles to maximize microbicide mucoadhesion

Chitosan‐triclosan particles modulate inflammatory signaling in gingival fibroblasts

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