Preparation of cholesterol oxidase nanoparticles and their application in amperometric determination of cholesterol

Applications of Enzyme Nanoparticles

“…An amperometric cholesterol 50 biosensor has been constructed based on ChOx NPs (Chawla et al, 2013). The following electrochemical reactions are involved in the measurement of response (current) of an amperometric cholesterol biosensor: The biosensor showed optimum response within 8 s at pH 6.0 and 35 C, when polarized at 0.27 V versus Ag/AgCl (Figure 6.8).…”

“…uric acid in biological fluids (Liu et al, 2005;Sharma et al, 2011;Kundu et al, 2012;Chawla et al, 2013;Chauhan et al, 2014).…”

Preparation of Enzyme Nanoparticles

“…Cross-linking of ENPs: The two aldehyde groups on both the ends of glutaraldehyde get attached to free ÀNH 2 group of lysine residue on the surface of ENPs through Schiff base formation as given below: The desolvation method has been employed for the preparation of NPs of few enzymes such as horseradish peroxidase (Liu et al, 2005), glucose oxidase from Aspergillus niger (Sharma et al, 2011;Kundu et al, 2012), cholesterol oxidase from microorganism sp. (Chawla et al, 2013) and uricase from Candida sp. expressed in Escherichia coli (Chauhan et al, 2014).…”

Introduction to Enzyme and Nanotechnology

“…Liu et al (2005) prepared and characterized NPs of horseradish peroxidase (HRP) for the first time. Thereafter, formation and application of ENPs of glucose oxidase (GOD), cholesterol oxidase (ChOx) and uricase in construction of amperometric biosensors were reported (Sharma et al, 2011;Kundu et al, 2012;Chawla et al, 2013;Chauhan et al, 2014).…”

“…The nanoparticles of the few enzymes such as HRP, GOD, ChOx and uricase have been prepared by desolvation and glutaraldehyde cross-linking method (Liu et al, 2005;Sharma et al, 2011;Kundu et al, 2012;Chawla et al, 2013;Chauhan et al, 2014) (Figure 1.1).…”