Applications of Enzyme Nanoparticles

Pundir, C.S.

doi:10.1016/b978-0-323-38913-6.00006-4

Cited by 5 publications

(5 citation statements)

References 5 publications

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“…Using the DPV method, we were able to show that 3D-NSCAs are superior to the laser-engraved graphene and the carbon nanotubes (CNTs) for UA electrocatalysis, with respect to current density (Figure H), thus establishing the high possibility of 3D-NSCAs as a type of cutting-edge nanoscaled material for developing sensitive sweat, saliva, or urine UA sensor. Through the incorporation of 3D-NSCAs into the sensing module (Figure S17), we obtain a broad linear connection between the UA content and the current with the dynamic ranges of 0.005–0.6 mM and 0.6–4.5 mM and a low detection limit of 1.42 μM (Figure I), which easily overlap the physiological sweat, saliva, and urine UA level ranges . In addition, the UA sensor is also highly selective for typical analytes found in human biofluids (Figure S18).…”

Section: Resultsmentioning

confidence: 95%

“…Through the incorporation of 3D-NSCAs into the sensing module (Figure S17), we obtain a broad linear connection between the UA content and the current with the dynamic ranges of 0.005−0.6 mM and 0.6−4.5 mM and a low detection limit of 1.42 μM (Figure 2I), which easily overlap the physiological sweat, 33 saliva, 31 and urine UA level ranges. 49 In addition, the UA sensor is also highly selective for typical analytes found in human biofluids (Figure S18). Because the UA sensor's response is highly affected by the sweat ionic strength (i.e., Na + level in this study) but not significantly sensitive to the variation of sweat pH (Figures S19 and S20), obtaining a precise evaluation of the raw sweat UA necessitates multiplexed Na + and UA sensing.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Universal Fully Integrated Wearable Sensor Arrays for the Multiple Electrolyte and Metabolite Monitoring in Raw Sweat, Saliva, or Urine

Sun

Wang

et al. 2023

Anal. Chem.

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Fully integrated wearable sensors are capable of dynamically, directly, and independently tracking biomarkers in raw noninvasive biofluids without any other equipment or accessories by integrating the unique on-body monitoring feature with the special complete functional implementation attribute. Sweat, saliva, and urine are three important noninvasive biofluids, and changes in their biomarkers hold great potential for revealing physiological conditions. However, it is still a challenge to design single fully integrated wearable sensor arrays (FIWSAs) that are universally able to concurrently measure electrolytes and metabolites in three of the most common noninvasive biofluids including sweat, saliva, and urine. Here, we propose the first single universal FIWSAs for wirelessly, noninvasively, and simultaneously measuring various metabolites (i.e., uric acid) and electrolytes (i.e., Na + and H + ) in raw sweat, saliva, or urine under subjects' exercise by integrating the specifically designed microfluidic, sensing, and electronic modules in a seamless manner. We evaluate its utility for noninvasive gout management in healthy subjects and in gout patients through a purine-rich meal challenge and with a medicine-treatment control, respectively. Noninvasive monitoring of multiple electrolytes and metabolites in a variety of raw noninvasive biofluids via such single universal FIWSAs may enrich the understanding of the biomarkers' levels in the body and would also facilitate self-health management.

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Section: Resultsmentioning

confidence: 95%

Section: ■ Results and Discussionmentioning

confidence: 99%

Universal Fully Integrated Wearable Sensor Arrays for the Multiple Electrolyte and Metabolite Monitoring in Raw Sweat, Saliva, or Urine

Sun

Wang

et al. 2023

Anal. Chem.

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“…In the case of uric acid at a concentration ratio of 1 : 100 (c spe : c inter ) the signal variation exceeded a value of 5 %. By considering the urine level of uric acid in healthy individuals of 3.68 mg dL −1 [44] and the appropriate dilution of the urine sample, it can be concluded that the concentration of uric acid affecting the selectivity of the developed method is three orders of magnitude higher than the expected in the urine sample. By excluding uric acid as a potential interferent, it has been proven that the developed SWV method using ZnONPs‐MWCNTs‐CPE as a sensor is selective for the determination of SPE.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical sensing platform based on the use of ZnONPs and MWCNTs as CPE modifiers for a selective and sensitive determination of polyamine spermine in the urine sample

et al. 2023

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A simple electroanalytical procedure has been developed for the determination of polyamine spermine using the ZnONPs‐MWCNTs‐CPE sensor. Spermine has been irreversibly oxidized on a modified electrode at a potential of +0.92 V versus Ag/AgCl (KCl, 3.5 mol L−1). The developed sensor demonstrated a respective linear response in borate buffer pH 8.5 from 2 μmol L−1 to 100 μmol L−1, with a detection limit of 0.300 μmol L−1 and quantification limit of 0.998 μmol L−1. The proposed sensor showed high reproducibility (RSD = 2.58 %), stability, robustness, and no obvious interference effects of several inorganic ions and organic molecules. The obtained results demonstrated excellent performance during the determination of the spermine in human urine samples with satisfactory recovery results (98.41–101.34 %), offering promising opportunities for practical clinical analysis.

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“… 81 Enzyme-based NPs attracted a lot of attention as biosensors with enhanced analytical performance and showed high potential as nano agents for delivering cargo. 82 , 83 …”

Section: Dye-loaded Protein-based Nanoparticles For Interventional Nimentioning

confidence: 99%

“…81 Enzyme-based NPs attracted a lot of attention as biosensors with enhanced analytical performance and showed high potential as nano agents for delivering cargo. 82,83 Although the above-mentioned types of protein-based NPs did not yet receive enough attention as candidates able to incorporate NIR dyes and serve as NIR fluorescence imaging agents, they display great potential for new research in this field.…”

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confidence: 99%

Interventional NIR Fluorescence Imaging of Cancer: Review on Next Generation of Dye-Loaded Protein-Based Nanoparticles for Real-Time Feedback During Cancer Surgery

Borlan

Focșan

Maniu

et al. 2021

IJN

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The use of fluorescence imaging technique for visualization, resection and treatment of cancerous tissue, attained plenty of interest once the promise of whole body and deep tissue near-infrared (NIR) imaging emerged. Why is NIR so desired? Contrast agents with optical properties in the NIR spectral range offer an upgrade for the diagnosis and treatment of cancer, by dint of the deep tissue penetration of light in the NIR region of the electromagnetic spectrum, also known as the optical window in biological tissue. Thus, the development of a new generation of NIR emitting and absorbing contrast agents able to overcome the shortcomings of the basic free dye administration is absolutely essential. Several examples of nanoparticles (NPs) have been successfully implemented as carriers for NIR dye molecules to the tumour site owing to their prolonged blood circulation time and enhanced accumulation within the tumour, as well as their increased fluorescence signal relative to free fluorophore emission and active targeting of cancerous cells. Due to their versatile structure, good biocompatibility and capability to efficiently load dyes and bioconjugate with diverse cancer-targeting ligands, the research area of developing protein-based NPs encapsulated or conjugated with NIR dyes is highly promising but still in its infancy. The current review aims to provide an up-to-date overview on the biocompatibility, specific targeting and versatility offered by protein-based NPs loaded with different classes of NIR dyes as next-generation fluorescent agents. Moreover, this study brings to light the newest and most relevant advances involving the state-of-the-art NIR fluorescent agents for the real-time interventional NIR fluorescence imaging of cancer in clinical trials.

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Applications of Enzyme Nanoparticles

Cited by 5 publications

References 5 publications

Universal Fully Integrated Wearable Sensor Arrays for the Multiple Electrolyte and Metabolite Monitoring in Raw Sweat, Saliva, or Urine

Universal Fully Integrated Wearable Sensor Arrays for the Multiple Electrolyte and Metabolite Monitoring in Raw Sweat, Saliva, or Urine

Electrochemical sensing platform based on the use of ZnONPs and MWCNTs as CPE modifiers for a selective and sensitive determination of polyamine spermine in the urine sample

Interventional NIR Fluorescence Imaging of Cancer: Review on Next Generation of Dye-Loaded Protein-Based Nanoparticles for Real-Time Feedback During Cancer Surgery

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