Preparation of chronic denervated gastric pouches in the rat

Abstract

A method is described for the surgical preparation, feeding and care of a chronic denervated gastric pouch in the rat. The basal secretion of the denervated pouch is approximately 0.24 ml/hr. and the hourly free HCl output is nearly zero after a fast of 12 hours. The volume of secretion and the free HCl output both increased after feeding with 3–5 gm of canned dog food. Insulin, 0.5 u, stimulated the output of free HCl from the innervated total gastric fistula but failed to stimulate the volume flow or the acid output of the denervated pouch. A denervated pouch prepared from the squamous or nonglandular portion of the stomach was also described. This portion of the stomach never secreted any free acid at any time over a period of 6 months postoperatively. This is the first physiologic proof that the white portion of the rat stomach is nonacid secreting. The advantages of using a denervated gastric pouch for physiologic and pharmacologic studies are discussed.

Gastric secretion during pregnancy and lactation in the rat

“…3 and Table 2. The interdigestive secretion of the pouches was absent or very scanty, thus confirming the observations of Alphin & Lin (1959), and did not change measurably during pregnancy and lactation. In one rat, not included in the series, there was a conspicuous increase in basal secretion during lactation, presumably due to reinnervation of the pouch.…”

Displacement by Metiamide of the Dose‐response Curves to Pentagastrin and Methacholine in the Conscious Rat

“…They were fed a standard pellet diet and tap water and when provided with pouches had the choice of Tyrode solution or tap water. Heidenhain pouches were prepared in the main according to Alphin & Lin (1959) (Svensson, 1970) and analysed for HCI by titration against 0.1 N NaOH with phenol red as an indicator. The secretory stimulants and metiamide (N-methyl-N' [ 2 [(5-methyl imidazol-4yl) Metiamide in a dose of 500 ;g/h reduced the observed and calculated maximal response to 64.1 ± 6.50 and 66.4 ± 7.40 ,Eq/30 min respectively.…”

Gastrin derivatives investigated for secretory potency and for changes in gastric mucosal histamine formation

“…Stomach preparation. Heidenhain pouches were prepared as described by Alphin & Lin (1959). Collection of gastric juice and determination of HCI and pepsin secretion were performed as described in detail elsewhere (Svensson, 1970).…”