Preparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability

Chen, Lifang; Huang, Yulang; Yang, Rongfeng; Xiao, Jian; Gao, Jiajia; Zhang, Debao; Cao, Duanwen; Xiao, Ke

doi:10.1080/21655979.2021.1982270

Cited by 4 publications

(1 citation statement)

References 47 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The viability of the growth factor payloads after electrospinning is evident by their ability to induce matrix-specific gene expression, as has been established by others as well. 58 – 60 The combination of the specific morphogen (BMP4 vs TGF-β1) appeared to promote a more osteogenic, perhaps osteodentin-like, rather than dentin phenotype that is currently being investigated. This may provide a future strategy where BMP actions could be blocked (e.g.…”

Section: Discussionmentioning

confidence: 99%

Precision-engineered niche for directed differentiation of MSCs to lineage-restricted mineralized tissues

et al. 2022

View full text Add to dashboard Cite

The major difference between tissue healing and regeneration is the extent of instructional cues available to precisely direct the biological response. A classic example is reparative or osteodentin that is seen in response to physicochemical injury to the pulp-dentin complex. Dentin regeneration can direct the differentiation of dental stem cells using concerted actions of both soluble (biomolecules, agonists, and antagonists) and insoluble (matrix topology) cues. The major purpose of this study was to examine the synergistic combination of two discrete biomaterial approaches by utilizing nanofiber scaffolds in discrete configurations (aligned or random) with incorporated polymeric microspheres capable of controlled release of growth factors. Further, to ensure appropriate disinfection for clinical use, Radio-Frequency Glow Discharge (RFGD) treatments were utilized, followed by seeding with a mesenchymal stem cell (MSC) line. SEM analysis revealed electrospinning generated controlled architectural features that significantly improved MSC adhesion and proliferation on the aligned nanofiber scaffolds compared to randomly oriented scaffolds. These responses were further enhanced by RFGD pre-treatments. These enhanced cell adhesion and proliferative responses could be attributed to matrix-induced Wnt signaling that was abrogated by pre-treatments with anti-Wnt3a neutralizing antibodies. Next, we incorporated controlled-release microspheres within these electrospun scaffolds with either TGF-β1 or BMP4. We observed that these scaffolds could selectively induce dentinogenic or osteogenic markers (DSPP, Runx2, and BSP) and mineralization. This work demonstrates the utility of a novel, modular combinatorial scaffold system capable of lineage-restricted differentiation into bone or dentin. Future validation of this scaffold system in vivo as a pulp capping agent represents an innovative dentin regenerative approach capable of preserving tooth pulp vitality.

show abstract

Section: Discussionmentioning

confidence: 99%

Precision-engineered niche for directed differentiation of MSCs to lineage-restricted mineralized tissues

et al. 2022

View full text Add to dashboard Cite

show abstract

Harnessing the Potential of Spider Silk Proteins for Biomedical Applications: from Native Silk Fibers to Designed Bioactive Materials

Fang,

Li,

Kou

et al. 2024

Adv Funct Materials

View full text Add to dashboard Cite

Spider silk has only been used anecdotally throughout human history, owing to limited supply from natural sources and recalcitrance toward biochemical functionalization. Recent advances in materials synthetic biology have ameliorated the challenges of mass‐producing spider silk and have thus paved the way for reengineering and redesigning these materials for real‐world applications. Here, an update is presented on the development of biomaterials comprising spider silk proteins within the past five years and the respective chemical and genetic approaches behind these developments. Potential applications are further highlighted in areas such as 3‐dimension (3D) cell culturing, drug delivery, theranostics, wound healing, tissue engineering, anti‐infection, and so on. By providing some glimpses into the latest innovations centered around spider silk proteins, as well as the challenges facing their biomedical applications, it is hoped that this will inspire more translational studies of these materials for real‐world impact.

show abstract

Engineered spidroin-derived high-performance fibers for diverse applications

Qin,

Li,

et al. 2023

Nano Res.

View full text Add to dashboard Cite

Preparation of controlled degradation of insulin-like growth factor 1/spider silk protein nanofibrous membrane and its effect on endothelial progenitor cell viability

Cited by 4 publications

References 47 publications

Precision-engineered niche for directed differentiation of MSCs to lineage-restricted mineralized tissues

Precision-engineered niche for directed differentiation of MSCs to lineage-restricted mineralized tissues

Harnessing the Potential of Spider Silk Proteins for Biomedical Applications: from Native Silk Fibers to Designed Bioactive Materials

Engineered spidroin-derived high-performance fibers for diverse applications

Contact Info

Product

Resources

About