2021
DOI: 10.1007/s00449-021-02509-7
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Preparation of cross-linked enzyme aggregates of lipase from Aspergillus niger: process optimization, characterization, stability, and application for epoxidation of lemongrass oil

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Cited by 23 publications
(5 citation statements)
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“…In the case of immobilized AA-ANL, the activity increased in the pH range of 4-7 and decreased at pH 8, with slight increase at pH 9. Compared with studies performed by other authors [60,86], the activity improvement with alkalization of the medium can be perceived as surprising, as authors had previously noted an activity decrease under these conditions. Our research confirmed the optimal pH for immobilized AA-ANL [60].…”
Section: The Effect Of Ph On the Lipolytic Activity Of Immobilized Aa...contrasting
confidence: 70%
“…In the case of immobilized AA-ANL, the activity increased in the pH range of 4-7 and decreased at pH 8, with slight increase at pH 9. Compared with studies performed by other authors [60,86], the activity improvement with alkalization of the medium can be perceived as surprising, as authors had previously noted an activity decrease under these conditions. Our research confirmed the optimal pH for immobilized AA-ANL [60].…”
Section: The Effect Of Ph On the Lipolytic Activity Of Immobilized Aa...contrasting
confidence: 70%
“…More recent work by Muley et al in 2021 [122] was based on the preparation of crosslinked enzymatic aggregates (CLEAs) of lipase from Aspergillus niger using fractional precipitation with ammonium sulfate and crosslinking with glutaraldehyde. In the end, lipase CLEAs showed better thermostability than its free form, in addition to retaining more than 65% of its activity for up to four cycles, exhibiting good storage stability for 12 days when stored at 4 ± 2 • C. Furthermore, they were successful in the application for epoxidation of lemongrass oil [122].…”
Section: Crosslinked Enzyme Aggregates (Cleas)mentioning
confidence: 98%
“…Reuse determines the number of cycles that it is possible to use the same enzyme and is essential for the industry, as with reuse process costs tend to decrease [34][35]. Usually, enzymatic activity decreases with each cycle performed.…”
Section: Reusementioning
confidence: 99%