Preparation of curcuminoid microemulsions from<i>Curcuma longa</i>L. to enhance inhibition effects on growth of colon cancer cells HT-29

Chen, Yen Chu; Chen, Bing Huei

doi:10.1039/c7ra12297g

Cited by 31 publications

(17 citation statements)

References 53 publications

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“…Fortunately, with the help of the ζ-potential value, the colloidal stability of the droplets can be demonstrated. The literature shows that because of electrostatic repulsion between nanoparticles, the highly stable μE droplets have a ζ-potential value of >30 or <−30 mV. ,,,− In this research, the ζ-potential of the EZM-free μE was −32.5 ± 1.4 mV, indicating the high stability of the μE. In contrast, a slightly higher negative ζ-potential (−38.7 ± 1.9 mV) of the EZM-loaded μE indicates its high stability.…”

Section: Resultsmentioning

confidence: 53%

Formation of Antihyperlipidemic Nano-Ezetimibe from Volatile Microemulsion Template for Enhanced Dissolution Profile

et al. 2020

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Nanostructures play an important role in targeting sparingly water-soluble drugs to specific sites. Because of the structural flexibility and stability, the use of template microemulsions (μEs) can produce functional nanopharmaceuticals of different sizes, shapes, and chemical properties. In this article, we report a new volatile oil-in-water (o/w) μE formulation comprising ethyl acetate/ethanol/brij-35/water to obtain the highly waterdispersible nanoparticles of an antihyperlipidemic agent, ezetimibe (EZM-NPs), to enhance its dissolution profile. A pseudoternary phase diagram was delineated in a specified brij-35/ethanol ratio (1:1) to describe the transparent, optically isotropic domain of the as-formulated μE. The water-dilutable μE formulation, comprising an optimum composition of ethyl acetate (18.0%), ethanol (25.0%), brij-35 (25.0%), and water (32.0%), showed a good dissolvability of EZM around 4.8 wt % at pH 5.2. Electron micrographs showed a fine monomodal collection of EZM-loaded μE droplets (∼45 nm) that did not coalesce even after lyophilization, forming small spherical EZM-NPs (∼60 nm). However, the maturity of nanodrug droplets observed through dynamic light scattering suggests the affinity of EZM to the nonpolar microenvironment, which was further supported through peak-to-peak correlation of infrared analysis and fluorescence measurements. Moreover, the release profile of the as-obtained EZM-nanopowder increased significantly >98% in 30 min, which indicates that a reduced drug concentration will be needed for capsules or tablets in the future and can be simply incorporated into the multidosage formulation of EZM.

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Section: Resultsmentioning

confidence: 53%

Formation of Antihyperlipidemic Nano-Ezetimibe from Volatile Microemulsion Template for Enhanced Dissolution Profile

et al. 2020

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“…Sharma et al reported an EE of 69.98 ± 0.21 to 83.32 ± 0.15% for curcumin-loaded Pluronic F-127 microemulsions [ 83 ]. Chen et al prepared curcuminoid microemulsions consisting of soybean oil, Tween 80, ethanol, and water with an EE of 85.7% [ 84 ]. Based on this study, this high EE percentage may be due to interactions between curcumin and microemulsions, which can result in a more rigid microemulsion membrane and affect curcumin release.…”

Section: Resultsmentioning

confidence: 99%

Biochemical, Ameliorative and Cytotoxic Effects of Newly Synthesized Curcumin Microemulsions: Evidence from In Vitro and In Vivo Studies

et al. 2021

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Curcumin is known to exhibit antioxidant and tissue-healing properties and has recently attracted the attention of the biomedical community for potential use in advanced therapies. This work reports the formulation and characterization of oil-in-water F127 microemulsions to enhance the bioavailability of curcumin Microemulsions showed a high encapsulation efficiency and prolonged release. To investigate the interactions of curcumin with one unit of the polymeric chain of surfactant F127, ethyl butyrate, and sodium octanoate, as well as the interaction between ethyl butyrate and one unit of the F127 polymer chain, the Density Functional Theory (DFT) calculations at the M06-2X level of theory, were performed in water solution. The MTT assay was used to assess the cytotoxicity of free and encapsulated curcumin on non-malignant and malignant cell lines. Combination effects were calculated according to Chou-Talalay’s principles. Results of in vitro studies indicated that MCF7 and HepG2 cells were more sensitive to curcumin microemulsions. Moreover, a synergistic relationship was observed between curcumin microemulsions and cisplatin in all affected fractions of MCF7 and HepG2 cells (CI < 0.9). For in vivo investigation, thioacetamide-intoxicated rats received thioacetamide (100 mg/kg Sc) followed by curcumin microemulsions (30 mg/kg Ip). Thioacetamide-intoxicated rats showed elevated serum liver enzymes, blood urea nitrogen (BUN), and creatinine levels, and a significant reduction in liver superoxide dismutase (SOD) and catalase (CAT) activities (p < 0.05). Curcumin microemulsions reduced liver enzymes and serum creatinine and increased the activity of antioxidant enzymes in thioacetamide-treated rats in comparison to the untreated thioacetamide-intoxicated group. Histopathological investigations confirmed the biochemical findings. Overall, the current results showed the desirable hepatoprotective, nephroprotective, and anti-cancer effects of curcumin microemulsions.

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“…Annexin V/PI assay can distinguish apoptotic and necrotic cells using both Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) stains, established on translocation of phosphatidylserine (PS) from the inner plasma membrane into outer cell surface in apoptotic cells where it can particularly bind with a fluorescent Annexin V -FITC, also PI can distinguish permeabilized plasma membrane of necrotic cells. Thus the percentages of apoptosis/necrosis cells in untreated SKOV3 cells and the treated SKOV3 ovarian cancer cells that were previously treated with an IC 50 concentration of SnO 2 NPs and FA-SnO 2 NPs for 24 h, was assessed by an Annexin V-FITC detection kit I (BD Biosciences) as previously mentioned [38,39]. Briefly,…”

Section: Lactate Dehydrogenase Leakage Test (Ldh)mentioning

confidence: 99%

Induction of mitochondria mediated apoptosis in human ovarian cancer cells by folic acid coated tin oxide nanoparticles

Hanna

Saad

2021

PLoS ONE

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Purpose This study aims to prepare folic acid coated tin oxide nanoparticles (FA-SnO2 NPs) for specifically targeting human ovarian cancer cells with minimum side effects against normal cells. Methods The prepared FA-SnO2 NPs were characterized by FT-IR, UV-vis spectroscopy, XRD, SEM and TEM. The inhibition effects of FA-SnO2 NPs against SKOV3 cancer cell were tested by MTT and LDH assay. Apoptosis induction in FA-SnO2 NPs treated SKOV3 cells were investigated using Annexin V/PI, AO/EB and Comet assays and the possible mechanisms of the cytotoxic action were studied by Flow cytometry, qRT-PCR, Immunohistochemistry, and Western blotting analyses. The effects of FA-SnO2 NPs on reactive oxygen species generation in SKOV3 cells were also examined. Additionally, the safety of utilization FA-SnO2 NPs were studied in vivo using Wister rats. Results The obtained FA-SnO2 NPs displayed amorphous spherical morphology with an average diameter of 157 nm and a zeta potential value of -24 mV. Comparing to uncoated SnO2 NPs, FA-SnO2 NPs had a superior inhibition effect towards SKOV3 cell growth that was suggested to be mediated through higher reactive oxygen species generation. It was showed that FA-SnO2 NPs increased significantly the % of apoptotic cells in the sub- G1 and G2/M phases with a higher intensity comet nucleus in SKOV3 treated cells. Furthermore, FA-SnO2 NPs was significantly increased the expression levels of P53, Bax, and cleaved Caspase-3 and accompanied with a significant decrease of Bcl-2 in the treated SKOV3 cells. Conclusion Overall, the results suggested that an increase in cellular FA-SnO2 NPs internalization resulted in a significant induced cytotoxicity in SKOV3 cancer cells in dose-dependent mode through ROS-mediated cell apoptosis that may have occurred through mitochondrial pathway. Additionally, the results confirmed the safety of utilization FA-SnO2 NPs against living systems. So, FA-SnO2 NPs with a specific targeting moiety may be a promising therapeutic candidate for human ovarian cancer.

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Preparation of curcuminoid microemulsions fromCurcuma longaL. to enhance inhibition effects on growth of colon cancer cells HT-29

Abstract: The objectives of this study were to extract curcuminoids from a vital medicinal plant Curcuma longa L. and prepare the curcuminoid extract and microemulsion for studying the inhibition mechanism of HT-29 colon cancer cells.

Cited by 31 publications

References 53 publications

Formation of Antihyperlipidemic Nano-Ezetimibe from Volatile Microemulsion Template for Enhanced Dissolution Profile

Formation of Antihyperlipidemic Nano-Ezetimibe from Volatile Microemulsion Template for Enhanced Dissolution Profile

Biochemical, Ameliorative and Cytotoxic Effects of Newly Synthesized Curcumin Microemulsions: Evidence from In Vitro and In Vivo Studies

Induction of mitochondria mediated apoptosis in human ovarian cancer cells by folic acid coated tin oxide nanoparticles

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