Preparation of Dextran Aldehyde and BSA Conjugates from Ligno-cellulosic Biowaste for Antioxidant and Anti-cancer Efficacy

Rai, Sudheer; Arun, S.; Kureel, Amit Kumar; Dutta, Pradip Kumar; Mehrotra, Gopal K.

doi:10.1007/s12649-020-01088-0

Cited by 8 publications

(4 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The authors also demonstrated that their lignin inhibited the activation of the nuclear transcription factor (NF-κB) in cancer cells. In a work of Rai et al (2021), kraft lignin was combined with dextran polysaccharide in treatment of the colon cancer cells. It was concluded that the combination of the lignin with other biopolymers can become an alternative chemotherapeutic approach for malignant tumor treatments.…”

Section: Potential Mechanism Of the Lnp Interaction With Liver Cancer...mentioning

confidence: 99%

High-Molecular-Weight Fractions of Spruce and Eucalyptus Lignin as a Perspective Nanoparticle-Based Platform for a Therapy Delivery in Liver Cancer

Pylypchuk

Suo

Chucheepchuenkamol

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

The natural polymer, lignin, possesses unique biodegradable and biocompatible properties, making it highly attractive for the generation of nanoparticles for targeted cancer therapy. In this study, we investigated spruce and eucalyptus lignin nanoparticles (designated as S-and E-LNPs, respectively). Both LNP types were generated from high-molecular-weight (Mw) kraft lignin obtained as insoluble residues after a five-step solvent fractionation approach, which included ethyl acetate, ethanol, methanol, and acetone. The resulting S-and E-LNPs ranged in size from 16 to 60 nm with uniform spherical shape regardless of the type of lignin. The preparation of LNPs from an acetone-insoluble lignin fraction is attractive because of the use of high-Mw lignin that is otherwise not suitable for most polymeric applications, its potential scalability, and the consistent size of the LNPs, which was independent of increased lignin concentrations. Due to the potential of LNPs to serve as delivery platforms in liver cancer treatment, we tested, for the first time, the efficacy of newly generated E-LNPs and S-LNPs in two types of primary liver cancer, hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA), in vitro. Both S-LNPs and E-LNPs inhibited the proliferation of HCC cells in a dose-dependent manner and did not affect CCA cell line growth. The inhibitory effect toward HCC was more pronounced in the E-LNP-treated group and was comparable to the standard therapy, sorafenib. Also, E-LNPs induced late apoptosis and necroptosis while inhibiting the HCC cell line. This study demonstrated that an elevated number of carbohydrates on the surface of the LNPs, as shown by NMR, seem to play an important role in mediating the interaction between LNPs and eukaryotic cells. The latter effect was most pronounced in E-LNPs. The novel S- and E-LNPs generated in this work are promising materials for biomedicine with advantageous properties such as small particle size and tailored surface functionality, making them an attractive and potentially biodegradable delivery tool for combination therapy in liver cancer, which still has to be verified in vivo using HCC and CCA models.

show abstract

Section: Potential Mechanism Of the Lnp Interaction With Liver Cancer...mentioning

confidence: 99%

High-Molecular-Weight Fractions of Spruce and Eucalyptus Lignin as a Perspective Nanoparticle-Based Platform for a Therapy Delivery in Liver Cancer

Pylypchuk

Suo

Chucheepchuenkamol

et al. 2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…The structural and physicochemical properties of materials can influence biological activities, including the ROS production and leading to ROS-induced damage . Recently, it was reported that lignin showed an anticancer activity to specific cell lines by generating intracellular ROS generation. ,, As a result, we want to investigate the ROS generation of our NPs and its relationship with cytotoxicity against cancer cells. LG-M(N)-PEG NPs without DOX and lignin with lignin-equivalent concentrations were incubated with 4T1 cells for different times.…”

Section: Resultsmentioning

confidence: 99%

Assessing the Potential of New Lignin-Based pH-Responsive Nanoparticles as Drug Carriers for Cancer Treatment

Yang

Zhou

et al. 2022

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

In this study, we prepared new lignin-based pH-responsive nanoparticles (LG-M(N)-PEG NPs) and their conjugates (LG-M(N-DOX)-PEG NPs) by using polyethylene glycol (PEG), doxorubicin (DOX), and alkaline lignin. In these NPs, the PEG chains were conjugated to lignin by an UV irradiated thiol–ene click reaction. The hydrazine and β-thiopropionate bonds in the NPs could conduct pH-triggered release of both DOX and lignin at an acidic pH in the tumor cells. Results showed that LG-M(N-DOX)-PEG NPs had a moderate particle size (48.3 ± 3.2 nm), significant cytotoxicity against 4T1 cells, and enhanced cellular uptake. Interestingly, the NPs without DOX (LG-M(N)-PEG NPs) could increase intracellular reactive oxygen species generation, induce cell pyroptosis, and result in a selective cytotoxicity to cancer cells. While the LG-M(N-DOX)-PEG NPs could deliver both lignin and DOX, they had favorable carrier-enhanced cytotoxicity and higher cancer cellular uptake compared to free DOX. Moreover, LG-M(N-DOX)-PEG NPs exhibited a clear tumor-inhibiting effect in vivo. Therefore, the NPs described here have great potential application in the drug delivery system.

show abstract

“…68 Lubna et al 69 used lignin as an important ingredient in an innovative and robust treatment of colon cancer. Sudheer et al 70 It was also found that even a small amount of lignin exhibited a significant cytotoxicity against colon cancer cells. It was concluded that the combination of lignin with other biopolymers improved the ideal repair effect, thus becoming alternative chemotherapeutic agents for the treatment of malignant tumors.…”

Section: Lignin In Tissue Therapymentioning

confidence: 98%

“…Lubna et al used lignin as an important ingredient in an innovative and robust treatment of colon cancer. Sudheer et al successfully utilized the bagass- based KL in the preparation of beneficial materials for cancer treatment. Through the free-radical reaction induced by the ascorbic acid/H 2 O 2 redox pair, a desired amount of KL was combined with dextran aldehyde (Dex-ald) and bovine serum albumin (BSA) for the synthesis of new conjugated materials Dex-ald–KL and BSA–KL with enhanced solubility.…”

Section: Lignin In Tissue Therapymentioning

confidence: 99%

Biological Activities and Emerging Roles of Lignin and Lignin-Based Products─A Review

et al. 2021

View full text Add to dashboard Cite

Bioactive substances, displaying excellent biocompatibility, chemical stability, and processability, could be extensively applied in biomedicine and tissue engineering. In recent years, plant-based bioactive substances such as flavonoids, vitamins, terpenes, and lignin have received considerable attention due to their human health benefits and pharmaceutical/medical applications. Among them is lignin, an amorphous biomacromolecule mainly derived from the combinatorial radical coupling of three phenylpropane units (p-hydroxypenyl, guaiacyl, and syringyl) during lignification. Lignin possesses intrinsic bioactivities (antioxidative, antibacterial, anti-UV activities, etc.) against phytopathogens. Lignin also enhances the plant resistance (adaptability) against environmental stresses. The abundant structural features of lignin offer other significant bioactivities including antitumor and antivirus bioactivities, regulation of plant growth, and enzymatic hydrolysis of cellulose. This Review reports the latest research results on the bioactive potential of lignin and lignin-based substances in biomedicine, agriculture, and biomass conversion. Moreover, the interfacial reactions and bonding mechanisms of lignin with biotissue/ cells and other constituents were also discussed, aiming at promoting the conversion or evolution of lignin from industrial wastes to value-added bioactive materials.

show abstract

Preparation of Dextran Aldehyde and BSA Conjugates from Ligno-cellulosic Biowaste for Antioxidant and Anti-cancer Efficacy

Cited by 8 publications

References 50 publications

High-Molecular-Weight Fractions of Spruce and Eucalyptus Lignin as a Perspective Nanoparticle-Based Platform for a Therapy Delivery in Liver Cancer

High-Molecular-Weight Fractions of Spruce and Eucalyptus Lignin as a Perspective Nanoparticle-Based Platform for a Therapy Delivery in Liver Cancer

Assessing the Potential of New Lignin-Based pH-Responsive Nanoparticles as Drug Carriers for Cancer Treatment

Biological Activities and Emerging Roles of Lignin and Lignin-Based Products─A Review

Contact Info

Product

Resources

About