Preparation of DNA–cyclodextrin–silica composite by sol–gel method and its utilization as an environmental material

Yamada, Masashi; Nakayama, Emiko

doi:10.1016/j.matchemphys.2012.01.022

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…Among available biomass feedstocks, nucleic acids (e.g., DNA, RNA) represent ubiquitous biomacromolecules readily available at a reasonable cost from fish milt, plants, yeasts, and other renewable sources. While the occurrence of nucleic acids is lower compared to polysaccharide types of biomass, and the adaptation of DNA for materials production is still in progress, potential applications of DNA in biomedical [ 3 , 4 , 5 ], environmental [ 6 , 7 , 8 , 9 , 10 ], electronic [ 11 ], optical [ 12 ], and engineering [ 13 , 14 , 15 ] fields have been repeatedly demonstrated. A very recent report introduced the concept of “DNA biomass” highlighting transformation of DNA to functional soft materials and even to biodegradable plastics [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Fluorescent Nanoparticles Synthesized from DNA, RNA, and Nucleotides

et al. 2021

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Ubiquitous on Earth DNA and other nucleic acids are being increasingly considered as promising biomass resources. Due to their unique chemical structure, which is different from that of a more common carbohydrate biomass polymers, materials based on nucleic acids may exhibit new attractive characteristics. In this study, fluorescent nanoparticles (biodots) were prepared by a hydrothermal (HT) method from various nucleic acids (DNA, RNA, nucleotides, and nucleosides) to establish the relationship between the structure of precursors and fluorescent properties of biodots and to optimize conditions for preparation of the most fluorescent product. HT treatment of nucleic acids results in decomposition of sugar moieties and depurination/depyrimidation of nucleobases, while their consequent condensation and polymerization gives fluorescent nanoparticles. Fluorescent properties of DNA and RNA biodots are drastically different from biodots synthesized from individual nucleotides. In particular, biodots synthesized from purine-containing nucleotides or nucleosides show up to 50-fold higher fluorescence compared to analogous pyrimidine-derived biodots. The polymeric nature of a precursor disfavors formation of a bright fluorescent product. The reported effect of the structure of the nucleic acid precursor on the fluorescence properties of biodots should help designing and synthesizing brighter fluorescent nanomaterials with broader specification for bioimaging, sensing, and other applications.

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Section: Introductionmentioning

confidence: 99%

Fluorescent Nanoparticles Synthesized from DNA, RNA, and Nucleotides

et al. 2021

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“…The research on synthetic bio-immobilization platforms has gained interest over the last decade. Several techniques including physisorption, 1–5 covalent attachment 6–9 and encapsulation 10–12 of different biomolecules on/in solid hosts have been developed for sensing and separating of bacteria, enzymes, cells and nucleic acids. In this respect, sol-gel encapsulation is a simple, low temperature process offering mechanically robust and chemically inert porous environment for preservation of biological activity and function of the biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Exploring encapsulation mechanism of DNA and mononucleotides in sol-gel derived silica

Kapusuz

Durucan

2017

J Biomater Appl

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The encapsulation mechanism of DNA in sol-gel derived silica has been explored in order to elucidate the effect of DNA conformation on encapsulation and to identify the nature of chemical/physical interaction of DNA with silica during and after sol-gel transition. In this respect, double stranded DNA and dAMP (2'-deoxyadenosine 5'-monophosphate) were encapsulated in silica using an alkoxide-based sol-gel route. Biomolecule-encapsulating gels have been characterized using UV-Vis, Si NMR, FTIR spectroscopy and gas adsorption (BET) to investigate chemical interactions of biomolecules with the porous silica network and to examine the extent of sol-gel reactions upon encapsulation. Ethidium bromide intercalation and leach out tests showed that helix conformation of DNA was preserved after encapsulation. For both biomolecules, high water-to-alkoxide ratio promoted water-producing condensation and prevented alcoholic denaturation. NMR and FTIR analyses confirmed high hydraulic reactivity (water adsorption) for more silanol groups-containing DNA and dAMP encapsulated gels than plain silica gel. No chemical binding/interaction occurred between biomolecules and silica network. DNA and dAMP encapsulated silica gelled faster than plain silica due to basic nature of DNA or dAMP containing buffer solutions. DNA was not released from silica gels to aqueous environment up to 9 days. The chemical association between DNA/dAMP and silica host was through phosphate groups and molecular water attached to silanols, acting as a barrier around biomolecules. The helix morphology was found not to be essential for such interaction. BET analyses showed that interconnected, inkbottle-shaped mesoporous silica network was condensed around DNA and dAMP molecules.

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Design of a DNA-Based Biomaterial by Sol-Gel Method: Application for the Recognition of Albendazole Sulfoxide

et al. 2019

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We describe the use of immobilized deoxyribonucleic acid (DNA) in a silica matrix as a biorecognition agent for the detection of albendazole sulfoxide (ASU), the primary metabolite of albendazole and a suspected teratogenic and embryotoxic agent. The biomaterial (DNA-containing gel) was synthesized by physical entrapment of salmon sperm in an inorganic silicate matrix by the sol-gel method. Functionality of the DNA-containing gel was evaluated by comparative offline frontal chromatography followed by HPLC analysis of ASU and caffeine (CAF, control) using DNA-containing gel and DNA-free gel. The DNA-containing gel showed relatively high specific retention for ASU, while CAF showed no retention using frontal analysis. We anticipate that the DNA-containing gel can be implemented to identify the interactions of DNA with other active pharmaceutical ingredients (APIs) and their metabolites in a readily available, sensitive and selective frontal chromatography experiment.

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Preparation of DNA–cyclodextrin–silica composite by sol–gel method and its utilization as an environmental material

Cited by 3 publications

References 25 publications

Fluorescent Nanoparticles Synthesized from DNA, RNA, and Nucleotides

Fluorescent Nanoparticles Synthesized from DNA, RNA, and Nucleotides

Exploring encapsulation mechanism of DNA and mononucleotides in sol-gel derived silica

Design of a DNA-Based Biomaterial by Sol-Gel Method: Application for the Recognition of Albendazole Sulfoxide

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