Preparation of dry flowable formulations of Clonostachys rosea by spray drying and application for Sclerotinia sclerotiorum control

Abstract

A dry flowable formulation of Clonostachys rosea with fungicidal activity against Sclerotinia sclerotiorum was prepared by spray drying. The formulation was optimized by a four-factor, three-level orthogonal experiment to screen inert ingredients and spray-drying conditions. The optimal dry flowable formulation of C. rosea included 30% C. rosea (ratio of conidia powder and its fermentation broth is 1:3), 3% Morwet EFW, 4% k12, 10% Morwet D425, 9% sodium salt of polynaphthalene sulphonic acid (NNO), 5% croscarmellose sodium, 5% (NH 4) 2 SO 4 , 0.5% carboxymethyl cellulose sodium (CMC-Na), 1% oxalic acid and palygorskite (carrier) up to 100%. The formulation exhibited good physical characteristics, such as high dispersibility, viability and a long shelf life. Plate antagonism tests and pot trials indicated that the dry flowable formulation was very effective against S. sclerotiorum, with control efficiency of up to 88.30%. This dry flowable formulation of C. rosea is a new potential commercial fungicide for spray drying to control S. sclerotiorum.

Ecology and diversity of culturable fungal species associated with soybean seedling diseases in the Midwestern United States

“…Clonostachys rosea— constituting 2% of the fungal isolates recovered in this study–has been reported to be a biological control agent with activity against important phytopathogens, including Sclerotinia sclerotiorum , F. graminearum , and R. solani (Gimeno et al, 2019; Wu et al, 2018; Karlsson et al, 2015; Salamone et al, 2018). However, C. rosea was also reported to be a potential pathogen, capable of causing root rot, interveinal chlorosis and marginal necrosis on soybean seedlings (Bienapfl et al, 2012).…”

Antagonistic fungi in the soil after Daucus carota L. cultivation

“…and Clonostachys spp. (Leelavathi et al 2014, Reddy et al 2014, Wu et al 2018. They produce antibiotics, siderophores and chitinolytic enzymes, which cause decomposition of cell walls and lysis of the mycelium (Sarma et al 2014, Smitha et al 2014.The purpose of the present studies was to determine the effect of intercrop plants (rye, buckwheat, white mustard and sunflower) used in carrot cultivation on the occurrence of Clonostachys spp., Albifimbria spp., Trichoderma spp.…”

“…and Clonostachys spp. (Leelavathi et al 2014, Reddy et al 2014, Wu et al 2018. They produce antibiotics, siderophores and chitinolytic enzymes, which cause decomposition of cell walls and lysis of the mycelium (Sarma et al 2014, Smitha et al 2014.…”

“…The studied antagonistic fungi were also effective towards different plant pathogens in the cultivation of root chicory with cover crops (Patkowska et al (Ondráčková et al 2013, Leelavathi et al 2014, Reddy et al 2014, Wu et al 2018).…”

Actinobactérias de biomas brasileiros: biodiversidade e potencial de uso na agricultura

“…O controle biológico de S. sclerotiorum começou ser pesquisado no final da década de 1970 quando a problemática fitossanitária do mofo branco adquiriu importância, atualmente existem mais de trinta espécies de fungos e bactérias conhecidas por suas ações inibitórias contra esse patógeno (Zeng, 2012). Dentre os fungos destacam-se as espécies micoparasitas de escleródios, que são pertencentes aos gêneros Trichoderma, Clonostachys e Coniothyrium (Jones et al, 2014;Wu et al, 2018) O filo Actinobactéria pertencentes à ordem Actinomycetales reúne um grande número de espécies que possuem destacado potencial biotecnológico (Cuesta et al, 2012). Esse grupo, constituído principalmente por bactérias filamentosas, Gram-positivas, com grande conteúdo de citosina e guanina (G+C mol%) em seu DNA (Doumbou et al, 2002), possuem grande diversidade morfológica e fisiológica.…”