Preparation of Epirubicin Aptasensor Using Curcumin as Hybridization Indicator: Competitive Binding Assay between Complementary Strand of Aptamer and Epirubicin

Abstract

The present work explains the fabrication of a novel electrochemical aptasensor for identifying and measuring the epirubicin (Epi) by using curcumin (Cur) as an anticancer electrochemical indicator. The aptasensor prepared by immobilizing the thiolated aptamer on the surface of graphite screen‐printed electrode modified with gold nanoparticles/functionalized multiwall carbon nanotubes, ionic liquid and chitosan nanocomposite (AuNPs/FMWCNTs‐IL‐Chit/SPE). To evaluate the willingness of aptamer to interaction with Epi in the presence of complementary strand DNA, competitive binding assay between the complementary strand of aptamer and Epi were used. Cur tends to bound to the grooves of two strands DNA. With increasing the concentration of Epi in the range of 0.007–7.0 μM, the peak current of Cur decreased, due to the formation of aptamer‐Epi complex and decreasing the amount of complementary strand DNA. Through the control experiments, we examined the response of fabricated aptasensor for some anticancer drugs, which have a structure similar to the Epi. The results showed that using the thiol‐terminated aptamer as a recognition layer led to a sensor with a high tendency for Epi compared to other anticancer drugs.

Advances in Exosome Analysis Methods with an Emphasis on Electrochemistry

“…In principle, the current intensity correlated with the targeted analyte concentration is measured by changing the potential. Depending on the modes of how the potential is applied, this method is divided into cyclic voltammetry (CV), linear sweep voltammetry (LSV), differential pulse voltammetry (DPV), and square wave voltammetry (SWV). − Generally, the CV and LSV methods provide qualitative information regarding electrochemical reactions, whereas the DPV and SWV methods evaluate the performance of biosensors such as sensitivity and selectivity. − The chronoamperometry and chronocoulometry methods measure current and charge variations over time at a constant potential. These methods are sensitive and do not require the modification of the aptamers with labels for electrochemical signaling.…”

Development of core-shell magnetic molecularly imprinted polymer-based electrochemical sensor for sensitive and selective detection of ezetimibe

“…Therefore, development of an analytical method, as well as the selection of an appropriate method for the pharmacokinetic evaluation of Eze in humans to quantify drug concentrations in plasma or serum samples taken at different times from individuals can be challenging. Electrochemical methods have been used for the analysis of pharmacological compounds [ 11 , 12 ], these methods offer the advantage of low cost, high sensitivity, and low limit of detection, fast response, direct analysis, and are portable. Therefore, electrochemical methods have become more popular as an alternate method for the analysis of pharmaceutical and biological samples.…”

Sensitive Electrochemical Detection of Tryptophan Using a Hemin/G-Quadruplex Aptasensor

“…Aptamers have many advantages over antibodies. Namely, aptamers have high stability in the presence of different parameters such as pressure, temperature, pH, in vitro synthesis, and labeling with different groups during synthesis [19]. It has been shown that some aptamers catalyze reactions similar to peroxidases [20][21][22][23].…”