Preparation of five high‐purity iridoid glycosides from <i>Gardenia jasminoides</i> Eills by molecularly imprinted solid‐phase extraction integrated with preparative liquid chromatography

Chen, Lizong; Wang, Rongyu; Cui, Li; Wang, Xiao; Wang, Leilei; Song, Fanyong; Ji, Wenhua

doi:10.1002/jssc.201800086

Cited by 14 publications

(4 citation statements)

References 38 publications

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“…Geniposidic acid is one of the representative active ingredients in Fructus Gardeniae [15,16], which was chosen as one of the quality control markers of this herb [17,18]. In recent years, researchers have demonstrated that geniposidic acid has better plasma absorption and tissue distribution in pathological states [19].…”

Section: Introductionmentioning

confidence: 99%

The proteomic analysis of anti-inflammatory effects of geniposidic acid in LPS induced THP1 monocytic cells

Wang¹,

Yu²,

Zhang³

et al. 2023

ScienceAsia

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People's health is adversely impacted by diseases caused by abnormal inflammation; therefore, more effective treatments as well as research into the underlying mechanisms are urgently required. According to our preliminary research, geniposidic acid exhibits anti-inflammatory properties. Even though geniposidic acid has been linked to inflammation and shows promise in the treatment of aberrant inflammatory illnesses, its exact antiinflammatory mechanism is unknown. To create in vitro cellular inflammatory models for 48 h, we employed THP-1 cells treated with PMA (phorbol 12-myristate 13-acetate) and LPS (lipopolysaccharide), which were then treated with geniposidic acid. These models were further validated by ELISA-based detection of the expression of the interleukins IL-1, IL-6, IL-8, and tumor necrosis factor TNF-α. The anti-inflammatory properties of geniposidic acid were investigated using proteomics based on TMT-LC-MS/MS analysis and bioinformatic analysis. Geniposidic acid can act as an anti-inflammatory agent in inflammatory cell models, according to the successfully established THP-1 cell inflammatory models. GO and KEGG analyses further established numerous bioprocesses, molecular activities, and signaling pathways related to the regulation of geniposidic acid. Additionally, western blot analysis confirmed that geniposidic acid can suppress inflammation by lowering the expression of proteins linked to inflammation such as CXCL10, PPM1H, and TLR5. After more thorough mechanistic, animal, and clinical research were included, the current study revealed that anti-inflammatory efficacy of geniposidic acid from the standpoint of proteomics made it a promising medicine to cure and prevent diseases caused by aberrant inflammation.

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Section: Introductionmentioning

confidence: 99%

The proteomic analysis of anti-inflammatory effects of geniposidic acid in LPS induced THP1 monocytic cells

Wang¹,

Yu²,

Zhang³

et al. 2023

ScienceAsia

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“…For example, finding six potential lactate dehydrogenase inhibitors from Belamcanda chinensis [13], and screening compounds from the leaves of Ligustrum lucidum Ait as potent neuraminidase inhibitors [14]. A molecularly imprinted SPE was used to concentrate a kind of compounds and it integrated with prep-HPLC for preparation of active iridoid glycosides from Gardenia jasminoides Eills [15].…”

Section: Introductionmentioning

confidence: 99%

Bioactivity‐guided separation of antifungal compounds by preparative high‐performance liquid chromatography

Liu

Dai

Jiang

et al. 2021

J of Separation Science

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Bioactivity-guided chromatographic methods are of great significance for the isolation of the active compounds in complex samples. In this study, four antifungal compounds were located by activity screening and successfully isolated from a microbial fermentation sample by preparative high-performance liquid chromatography. Separation performance of columns including C18, positively charged C18, negatively charged C18 and C8 were firstly investigated. And it showed a better capacity of mixed-mode stationary phases for retention and separation. Therefore, the positively charged C18 column was used to separate the sample into several fractions, among which the active one was identified by the antifungal test. And then the active fraction was enriched and separated again by successively using the negatively charged C18 and C8 columns to obtain four compounds, which were identified as polyoxins A, K, F and H. With activity verification, four polyoxins were found to have good inhibitory effects against the three fungal plant diseases including rice sheath blight, tomato grey mould disease, and apple spot leaf disease.

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“…Molecularly imprinted polymers (MIPs) are prepared by using MIT with tailor‐made recognition sites for a given analyte and its structural analogs . MIPs present high specificity, desired selectivity, thermal stability, as well as low costs and easy preparation, which make them ideal materials for separation science . In the preparation process, MIPs are always obtained by bulk polymerization, precipitation polymerization, and suspension polymerization .…”

Section: Introductionmentioning

confidence: 99%

Preparation and evaluation of temperature and magnetic dual‐responsive molecularly imprinted polymers for the specific enrichment of formononetin

Zhang

Tan

et al. 2018

J of Separation Science

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Thermo-responsive magnetic molecularly imprinted polymers were prepared by simple surface molecular imprinting polymerization for the selective adsorption and enrichment of formononetin from Trifolium pretense by temperature regulation. Using formononetin as a template, N-isopropylacrylamide as the thermo-responsive functional monomer, and methacrylic acid as an assisting functional monomer, the polymers were synthesized on the surface of the magnetic substrate. The results show that imprinted polymers attained controlled adsorption of formononetin in response to the temperature change, with large adsorption capacity (16.43 mg/g), fast kinetics (60 min) and good selectivity at 35°C compared with that at 25 and 45°C. The selectivity experiment indicated that the materials had excellent recognition ability for formononetin and the selectivity factors were between 1.32 and 2.98 towards genistein and daidzein. The excellent linearity was attained in the range of 5-100 μg/mL, with low detection limits and low quantitation limits of 0.017 and 0.063 μg/mL, respectively. Furthermore, the thermo-responsive magnetic molecularly imprinted polymers were successfully utilized for enriching and purifying formononetin from Trifolium pretense. The analytical results indicate that the imprinted polymers are promising materials for selective identification and enrichment of formononetin in complicated herbal medicines by simple temperature-responsive regulation.

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Preparation of five high‐purity iridoid glycosides from Gardenia jasminoides Eills by molecularly imprinted solid‐phase extraction integrated with preparative liquid chromatography

Cited by 14 publications

References 38 publications

The proteomic analysis of anti-inflammatory effects of geniposidic acid in LPS induced THP1 monocytic cells

The proteomic analysis of anti-inflammatory effects of geniposidic acid in LPS induced THP1 monocytic cells

Bioactivity‐guided separation of antifungal compounds by preparative high‐performance liquid chromatography

Preparation and evaluation of temperature and magnetic dual‐responsive molecularly imprinted polymers for the specific enrichment of formononetin

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