Preparation of Formalinized Erythrocytes

Csizmas, L

doi:10.3181/00379727-103-25444

Cited by 93 publications

(30 citation statements)

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“…Hemagglutination titers were determined by standard microhemagglutination methods using a formatinized tanned sheep ceil assay (14,15). All scra from a single bleeding were run on the same day; positive sera of known titer and known negative sera were included with each assay.…”

Section: Materials and Metkodsmentioning

confidence: 99%

Relative Inability to Induce Tolerance in Adult NZB and NZB/NZW F1 Mice

Staples

Talal

1969

The Journal of Experimental Medicine

129

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Immunologic tolerance to ultracentrifuged bovine gamma globulin could not be induced in 6-wk old NZB or B/W mice, but developed readily in C3H, NZW, and C57Bl mice. NZB and B/W mice, as well as Balb/c mice, failed to become tolerant to ultracentrifuged human gamma globulin. The NZB and B/W mice also showed higher antibody titers to a standard antigenic challenge. This immunologic hyperreactivity and lack of experimental tolerance may be related to the lack of self-tolerance, autoimmunity, and lymphomas that develop in these mice at a later age.

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Section: Materials and Metkodsmentioning

confidence: 99%

Relative Inability to Induce Tolerance in Adult NZB and NZB/NZW F1 Mice

Staples

Talal

1969

The Journal of Experimental Medicine

129

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“…Sheep erythrocytes collected in Alsever's solution were formalinized according to the method of Csizmas (1960). The Formalinized sheep erythrocytes (FSE) are stored frozen in small aliquots until needed.…”

mentioning

confidence: 99%

Nutritional Requirements of Enterotoxigenic Strains of Staphylococcus Aureus

Mah¹

1965

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“…SRBC in Alsever's solution were formalinized by placing washed cells in a 10% solution of phosphate buffered saline in a graduated cylinder with a stirring bar as described by Csizmas (16). Twice the packed red cell volume of 40% formaldehyde was placed in a dialysis bag in the cylinder.…”

Section: Methodsmentioning

confidence: 99%

Rheumatoid factor inhibition of in vitro binding of IgG complexes in the human glomerulus

Bolton

Schrock

Davis

1982

Arthritis & Rheumatism

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We studied the effects of rheumatoid factor (RF) on binding of immune complexes to activated C3 (C3b) receptors in vitro. IgM fraction of serum containing RF activity (IgM‐RF), IgM isolated from pooled normal human serum and having no RF activity (IgM‐control), bovine serum albumin, and Veronal buffered saline solutions were used in a C3b assay system consisting of aggregated human IgG (AggHuIgG) coupled to sheep erythrocytes (SRBC) with guinea pig and normal human serum complement. The number of glomerular bound AggHuIgG‐SRBC with IgM‐control and bovine serum albumin or Veronal buffered saline was similar, while the number of bound cells with IgM‐RF was reduced significantly. This effect was seen with both guinea pig and normal human serum complements. Supernatant hemolytic complement activity was maintained with IgM‐RF, but reduced with control solutions. The blocking factor was shown to be RF by serial dilutions of IgM‐RF resulting in inverse correlations with latex flocculation and inhibition of SRBC binding, absorption of blocking from IgM‐RF with insolubilized AggHuIgG, and failure of IgM‐control to block binding. IgM‐RF did not directly interfere with activation of complement, but blocked attachment of C3 to AggHuIgG and formation of C3b capable of reacting with glomerular receptors. These results showed that IgM‐RF can inhibit binding of AggHuIgG complexes to human glomeruli. This in vitro phenomenon may represent a possible protective mechanism of RF in vivo in diseases with immune complexes.

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Preparation of Formalinized Erythrocytes

Cited by 93 publications

References 0 publications

Relative Inability to Induce Tolerance in Adult NZB and NZB/NZW F1 Mice

Relative Inability to Induce Tolerance in Adult NZB and NZB/NZW F1 Mice

Nutritional Requirements of Enterotoxigenic Strains of Staphylococcus Aureus

Rheumatoid factor inhibition of in vitro binding of IgG complexes in the human glomerulus

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