Preparation of Functional Magnetic Nanoparticles Mediated with PEG-4000 and Application in &lt;I&gt;Pseudomonas Aeruginosa&lt;/I&gt; Rapid Detection

Tang, Yongjun; Li, Zhiyang; He, Nongyue; Zhang, Liming; Ma, Chao; Li, Xiaolong; Li, Chuanyan; Wang, Zhifei; Deng, Yan; He, Lei

doi:10.1166/jbn.2013.1493

Cited by 41 publications

(21 citation statements)

References 43 publications

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“…A tube containing the reaction mixture and sterile water was included in all reactions as a negative control. The PCR products were analysed by electrophoresis (1.5 % agarose gel) 22-24.…”

Section: Methodsmentioning

confidence: 99%

Highly Sensitive and Rapid Detection of Pseudomonas aeruginosa Based on Magnetic Enrichment and Magnetic Separation

Tang¹,

Zou²,

Ma³

et al. 2013

Theranostics

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A method for highly sensitive and rapid detection of Pseudomonas aeruginosa, based on magnetic enrichment and magnetic separation, is described in this paper. The magnetic nanoparticles (MNPs) were applied to adsorb genome DNA after the sample was lysed. The DNA binding MNPs were directly subjected to polymerase chain reaction (PCR) to amplify gyrB specific sequence of Pseudomonas aeruginosa. The biotin labeled PCR products were detected by chemiluminescence when they were successively incubated with the probes-modified MNPs and alkaline phosphatase (ALP) labeled streptavidin (SA). Agarose gel electrophoresis analyses approved the method of in situ PCR to be highly reliable. The factors which could affect the chemiluminiscence were studied in detail. The results showed that the MNPs of 400 nm in diameter are beneficial to the detection. The sequence length and the binding site of the probe with a target sequence have obvious effects on the detection. The optimal concentration of the probes, hybridization temperature and hybridization time were 10 μM, 60 ºC and 60 mins, respectively. The method of in situ PCR based on MNPs can greatly improve the utilization rate of the DNA template ultimately enhancing the detection sensitivity. Experiment results proved that the primer and probe had high specificity, and Pseudomonas aeruginosa was successfully detected with detection limits as low as 10 cfu/mL by this method, while the detection of a single Pseudomonas aeruginosa can also be achieved.

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“…A tube containing the reaction mixture and sterile water was included in all reactions as a negative control. The PCR products were analysed by electrophoresis (1.5 % agarose gel) 22-24.…”

Section: Methodsmentioning

confidence: 99%

Highly Sensitive and Rapid Detection of Pseudomonas aeruginosa Based on Magnetic Enrichment and Magnetic Separation

Tang¹,

Zou²,

Ma³

et al. 2013

Theranostics

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“…Functionalization of the CNTs with magnetic Fe 3 O 4 nanoparticles (NPs) has been suggested to be very important in developing the targeting ability of the oxidized-CNTs. The multifunctional properties of the Fe 3 O 4 NPs, such as small size, high magnetic responsivity and low toxicity, [34][35][36][37][38][39][40][41][42][43] can be used to increase the accumulation of drugs in pathological sites, thereby reducing the non-specific toxicity and some other adverse side-effects. We therefore decorated the MWNTs with Fe 3 O 4 NPs to ensure a strong magnetic responsiveness and provide the targeted property for various applications.…”

Section: Introductionmentioning

confidence: 99%

Chelerythrine and Fe₃O₄ Loaded Multi-Walled Carbon Nanotubes for Targeted Cancer Therapy

Cao¹,

Liang²,

Zhao³

et al. 2016

j biomed nanotechnol

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The work focused on manufacturing improved drug loaded multifunctional magnetic nanoparticles that can overcome the relative non-specificity and potential side-effects of some chemotherapeutic drugs to healthy tissues. A new drug delivery system, Chelerythrine (CHE) and Fe3O4 loaded multi-walled carbon nanotubes (Fe3O4/MWNTs-CHE nanocomposites) that can target hepatocytes when treating malignant tumors, was prepared through a simple adsorption method. The formulation and structure of the Fe3O4/MWNTs-CHE nanocomposites were characterized by vibrating sample magnetometer (VSM), Fourier Transform infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The cytotoxicity and anti-proliferation effect from the prepared nanocomposites were in vitro tested on human hepatocarcinoma HepG2 and normal liver LO2 cell lines. The results showed the saturated magnetization of Fe3O4/MWNTs-CHE nanocomposites could reach to 45.4O3 emu/g, and the in vitro CHE release behavior exhibited a biphasic release pattern. Moreover, the in vitro cytotoxicity studies revealed that the Fe3O4/MWNTs-CHE nanocomposites showed an efficient inhibition rate to HepG2 cell line and exhibited a lower cytotoxicity to LO2 cell line in comparison to the native CHE. Therefore, the multifunctional Fe3O4/MWNTs-CHE nanocomposites should be a useful and promising candidate for treatment of malignant tumors.

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“…[5][6][7][8][9][10] Besides these, multiple reports have found that vd-siRNAs could also target host mRNA in a sequence-specific manner, resulting Copyright: American Scientific Publishers in silencing of the homologous host genes and subsequent viral symptom development. [11][12][13][14][15][16][17][18] For example, vdsiRNAs derived from the Cauliflower mosaic virus 35S leader sequence could specifically down-regulate the Arabidopsis At1g76950 transcript. 19 VsiRNAs from Tobacco mosaic virus Cg could specifically cleave At1g30460 and At2g16595 transcripts; 20 Vd-siRNAs from the Y-satellite of Cucumber mosaic virus (CMV) could specifically cleave the Nicotiana benthamiana ChlI mRNA and resulting in bright yellow symptoms.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Functional Analysis of Viral siRNAs in Tomato Infected with <I>Cucumber Mosaic Virus</I>

Feng¹,

Li²,

Chen³

2016

j nanosci nanotechnol

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A Large number of short interfering RNAs (siRNAs) are generated from plant viruses during infection, but their function, character and biogenesis are unclear. Here, we analyzed the viral small RNA profile in tomato plants upon Cucumber mosaic virus (CMV) Fny strain infection, using highthroughput deep sequencing techniques. A set of 129666 CMV-Fny-specific small RNAs, most of the 21-(54.6%) and 22-nucleotide (28.2%) in size were obtained. Both strands of CMV-Fny were substrates for production of viral-specific siRNAs, and the virus genome harbored multiple, highly reproducible small RNA-generating hot spots that corresponded to regions with no apparent local hairpin-forming capacity. In addition, using the novel high throughput degradom sequencing approach and informatics analysis, we found a large set of host genes could be potentially targeted by CMV-Fny-derived siRNAs for posttranscriptional silencing. Six of such host targets were further validated by qRT-PCR. Gene ontology analysis with biological process, cellular component and molecular function terms revealed that these target genes mainly related to stress response, cell redox homeostasis, protein folding/stabilization/transport, chloroplast-and photosynthesis-related processes, metal ion binding, ATP binding, jasmonic acid mediated signaling pathway, and gene silencing by RNA. These results indicate that the production of viral derived siRNAs might be involved in virus-host interactions, and could contribute to explain how pathogens cause symptoms in their hosts.

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Preparation of Functional Magnetic Nanoparticles Mediated with PEG-4000 and Application in <I>Pseudomonas Aeruginosa</I> Rapid Detection

Cited by 41 publications

References 43 publications

Highly Sensitive and Rapid Detection of Pseudomonas aeruginosa Based on Magnetic Enrichment and Magnetic Separation

Highly Sensitive and Rapid Detection of Pseudomonas aeruginosa Based on Magnetic Enrichment and Magnetic Separation

Chelerythrine and Fe₃O₄ Loaded Multi-Walled Carbon Nanotubes for Targeted Cancer Therapy

Characterization and Functional Analysis of Viral siRNAs in Tomato Infected with <I>Cucumber Mosaic Virus</I>

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Preparation of Functional Magnetic Nanoparticles Mediated with PEG-4000 and Application in <I>Pseudomonas Aeruginosa</I> Rapid Detection

Cited by 41 publications

References 43 publications

Highly Sensitive and Rapid Detection of Pseudomonas aeruginosa Based on Magnetic Enrichment and Magnetic Separation

Highly Sensitive and Rapid Detection of Pseudomonas aeruginosa Based on Magnetic Enrichment and Magnetic Separation

Chelerythrine and Fe3O4 Loaded Multi-Walled Carbon Nanotubes for Targeted Cancer Therapy

Characterization and Functional Analysis of Viral siRNAs in Tomato Infected with <I>Cucumber Mosaic Virus</I>

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Product

Resources

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Chelerythrine and Fe₃O₄ Loaded Multi-Walled Carbon Nanotubes for Targeted Cancer Therapy