Preparation of functionalized magnetic nanoparticles conjugated with feroxamine and their evaluation for pathogen detection

Martínez-Matamoros, Diana; Castro‐García, Socorro; Balado, Miguel; Matamoros-Veloza, Adriana; Camargo‐Valero, Miller Alonso; Céspedes, Oscar; Rodrı́guez, Jaime; Lemos, Manuel L.; Jiménez, Carlos

doi:10.1039/c8ra10440a

Cited by 12 publications

(5 citation statements)

References 34 publications

(41 reference statements)

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“…Bacteria enriched with van-PDA-MNPs were washed twice with PBS and the MNPs were separated on a magnetic separation rack. Fixation of the bacteria-MNP complexes was done in 2% glutaraldehyde solution at RT for 1 h [ 36 , 37 ]. Bacteria-MNP complexes were washed three times with 1 mL PBS and incubated in 1% osmium tetroxide for 1 h at 4 °C in the dark.…”

Section: Methodsmentioning

confidence: 99%

Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood

Abafogi

Wu²,

Lee

et al. 2022

J Nanobiotechnol

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Background Sepsis is caused mainly by infection in the blood with a broad range of bacterial species. It can be diagnosed by molecular diagnostics once compounds in the blood that interfere with molecular diagnostics are removed. However, this removal relies on ultracentrifugation. Immunomagnetic separation (IMS), which typically uses antibody-conjugated silica-coated magnetic nanoparticles (Ab-SiO2-MNPs), has been widely applied to isolate specific pathogens in various types of samples, such as food and environmental samples. However, its direct use in blood samples containing bacteria is limited due to the aggregation of SiO2-MNPs in the blood and inability to isolate multiple species of bacteria causing sepsis. Results In this study, we report the synthesis of vancomycin-conjugated polydopamine-coated (van-PDA-MNPs) enabling preconcentration of multiple bacterial species from blood without aggregation. The presence of PDA and van on MNPs was verified using transmission electron microscopy, X-ray photoelectron spectroscopy, and energy disruptive spectroscopy. Unlike van-SiO2-MNPs, van-PDA-MNPs did not aggregate in the blood. Van-PDA-MNPs were able to preconcentrate several species of Gram-positive bacteria in the blood, lowering the limit of detection (LOD) to 10 colony forming units/mL by polymerase chain reaction (PCR) and quantitative PCR (qPCR). This is 10 times more sensitive than the LOD obtained by PCR and qPCR using van-SiO2-MNPs. Conclusion These results suggest that PDA-MNPs can avoid aggregation in blood and be conjugated with receptors, thereby improving the sensitivity of molecular diagnostics of bacteria in blood samples.

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Section: Methodsmentioning

confidence: 99%

Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood

Abafogi

Wu²,

Lee

et al. 2022

J Nanobiotechnol

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“…The main problem associated with this method involves the blockage material for non-specific binding, the development of new blockage materials, and validity or updating of the method. 67 A high speed, ease of use and high-throughput analysis make these techniques superior to other identification methods, which can be used to detect Yersinia spp. However, these methods need to be further modified to reduce their high costs and long sample preparation requirements for routine testing.…”

Section: Molecular Typing Methodsmentioning

confidence: 99%

A comprehensive review on the prevalence, pathogenesis and detection ofYersinia enterocolitica

et al. 2019

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“…A low-intensity band at 610 cm −1 in the MNPs-MPTS is characteristic of SiO 2 vibrations. The weak S–H stretching band appears near 2500 cm −1 49 .The appearance of a shoulder at 760 cm −1 in the MNPs-APTS spectrum is related to Si–O–Si vibrations and confirmed the presence of APTS 50 . The peak at 1900 cm −1 , attributed to the C–C stretching vibrations in the APTS and MPTS ligands, also confirm functionalization 51 .…”

Section: Resultsmentioning

confidence: 55%

Impact of immobilization strategies on the activity and recyclability of lipases in nanomagnetic supports

Silva

Keijok

Guimarães

et al. 2022

Sci Rep

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The use of enzymes immobilized on nanomagnetic supports has produced surprising results in catalysis, mainly due to the increase in surface area and the potential for recovery and reuse. However, the meticulous control of the process and difficulties in reproducibility have made industrial-scale applications unfeasible. Furthermore, the role of conjugation strategies in the catalytic activity and recycling of catalysts is unclear. Therefore, the objective of this study was to compare the conjugation of enzymes on nanomagnetic supports through physical adsorption (naked) or covalent bonding with mercaptopropyltrimethoxysilane (MPTS) and aminopropyltriethoxysilane (APTS) ligands. The free lipase obtained from Rhizomucor miehei was used as a model enzyme. Total protein and enzyme activity were determined using spectrophotometry (UV–Vis) and the p-nitrophenyl palmitate (p-NPP) hydrolysis method. The results indicated that a more significant enzyme surface loading does not always mean better immobilization success. The physical adsorption binding strategy had higher surface loading and low catalytic activity. On the other hand, covalent coupling with free NH2 had an excellent catalytic activity with very low surface loading. Finally, we show that recyclability can be improved with conjugation mediated by disulfide bonds. The findings presented here are essential for developing nanoconjugates with high enzymatic activity, which can guarantee the success of several industrial applications.

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Preparation of functionalized magnetic nanoparticles conjugated with feroxamine and their evaluation for pathogen detection

Abstract: Interaction of a conjugate between amino-functionalized silica magnetite and the siderophore feroxamine with Yersinia enterocolitica wild type.

Cited by 12 publications

References 34 publications

Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood

Vancomycin-conjugated polydopamine-coated magnetic nanoparticles for molecular diagnostics of Gram-positive bacteria in whole blood

A comprehensive review on the prevalence, pathogenesis and detection ofYersinia enterocolitica

Impact of immobilization strategies on the activity and recyclability of lipases in nanomagnetic supports

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